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. 1978 Jan;19(1):194–198. doi: 10.1128/iai.19.1.194-198.1978

Cell-mediated immune responses of guinea pigs to an inactivated phase I Coxiella burnetii vaccine.

R A Kishimoto, J W Johnson, R H Kenyon, M S Ascher, E W Larson, C E Pedersen Jr
PMCID: PMC414066  PMID: 624586

Abstract

The ability of a killed phase I Coxiella burnetii vaccine to induce cell-mediated immune responses in guinea pigs was studied. Cell-mediated immune responses were assessed by the inhibition of macrophage migration and lymphocyte transformation assays. The macrophage migration response occurred rapidly and was detected at high levels, but was relatively short-lived. In contrast, the lymphocyte transformation response developed more slowly, and persisted for a longer period. The vaccine, given in a single dose or in two doses 1 week apart, protected guinea pigs from a subsequent virulent challenge.

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Selected References

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  1. ABINANTI F. R., MARMION B. P. Protective or neutralizing antibody in Q fever. Am J Hyg. 1957 Sep;66(2):173–195. doi: 10.1093/oxfordjournals.aje.a119894. [DOI] [PubMed] [Google Scholar]
  2. BENENSON A. S., TIGERTT W. D. Studies on Q fever in man. Trans Assoc Am Physicians. 1956;69:98–104. [PubMed] [Google Scholar]
  3. BOZEMAN F. M., ELISBERG B. L. Serological diagnosis of scrub typhus by indirect immunofluorescence. Proc Soc Exp Biol Med. 1963 Mar;112:568–573. doi: 10.3181/00379727-112-28107. [DOI] [PubMed] [Google Scholar]
  4. Coonrod J. D., Shepard C. C. Lymphocyte transformation in rickettsioses. J Immunol. 1971 Jan;106(1):209–216. [PubMed] [Google Scholar]
  5. Fiset P., Ormsbee R. A., Silberman R., Peacock M., Spielman S. H. A microagglutination technique for detection and measurement of rickettsial antibodies. Acta Virol. 1969 Jan;13(1):60–66. [PubMed] [Google Scholar]
  6. Fowles R. E., Fajardo I. M., Leibowitch J. L., David J. R. The enhancement of macrophage bacteriostasis by products of activated lymphocytes. J Exp Med. 1973 Oct 1;138(4):952–964. doi: 10.1084/jem.138.4.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hinrichs D. J., Jerrells T. R. In vitro evaluation of immunity to Coxiella burnetii. J Immunol. 1976 Sep;117(3):996–1003. [PubMed] [Google Scholar]
  8. Jerrells T. R., Mallavia L. P., Hinrichs D. J. Detection of long-term cellular immunity to Coxiella burneti as assayed by lymphocyte transformation. Infect Immun. 1975 Feb;11(2):280–286. doi: 10.1128/iai.11.2.280-286.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kenyon R. H., Ascher M. S., Kishimoto R. A., Pedersen C. E., Jr In vitro guinea pig leukocyte reactions to Rickettsia rickettsii. Infect Immun. 1977 Dec;18(3):840–846. doi: 10.1128/iai.18.3.840-846.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kishimoto R. A., Burger G. T. Appearance of cellular and humoral immunity in guinea pigs after infection with Coxiella burnetii administered in small-particle aerosols. Infect Immun. 1977 May;16(2):518–521. doi: 10.1128/iai.16.2.518-521.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kishimoto R. A., Veltri B. J., Shirey F. G., Canonico P. G., Walker J. S. Fat of Coxiella burnetti in macrophages from immune guinea pigs. Infect Immun. 1977 Feb;15(2):601–607. doi: 10.1128/iai.15.2.601-607.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kishimoto R. A., Walker J. S. Interaction between Coxiella burnetii and guinea pig peritoneal macrophages. Infect Immun. 1976 Aug;14(2):416–421. doi: 10.1128/iai.14.2.416-421.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee J. C., Sigel M. M. A differential effect of IgM and IgG antibodies on the blastogenic response of lymphocytes to rubella virus. Cell Immunol. 1974 Jul;13(1):22–31. doi: 10.1016/0008-8749(74)90223-8. [DOI] [PubMed] [Google Scholar]
  14. MEIKLEJOHN G., LENNETTE E. H. Q fever in California. I. Observations on vaccination of human beings. Am J Hyg. 1950 Jul;52(1):54–64. [PubMed] [Google Scholar]
  15. Nathan C. F., Karnovsky M. L., David J. R. Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971 Jun 1;133(6):1356–1376. doi: 10.1084/jem.133.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. ORMSBEE R. A., BELL E. J., LACKMAN D. B., TALLENT G. THE INFLUENCE OF PHASE ON THE PROTECTIVE POTENCY OF Q FEVER VACCINE. J Immunol. 1964 Mar;92:404–412. [PubMed] [Google Scholar]
  17. Oppenheim J. J. Modulation of in vitro lymphocyte transformation by antibodies: enhancement by antigen-antibody complexes and inhibition by antibody excess. Cell Immunol. 1972 Mar;3(3):341–360. doi: 10.1016/0008-8749(72)90243-2. [DOI] [PubMed] [Google Scholar]
  18. Robinson D. M., Hasty S. E. Production of a potent vaccine from the attenuated M-44 strain of Coxiella burneti. Appl Microbiol. 1974 Apr;27(4):777–783. doi: 10.1128/am.27.4.777-783.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Simon H. B., Sheagren J. N. Enhancement of macrophage bactericidal capacity by antigenically stimulated immune lymphocytes. Cell Immunol. 1972 Jun;4(2):163–174. doi: 10.1016/0008-8749(72)90015-9. [DOI] [PubMed] [Google Scholar]
  20. Spicer D. S., DeSanctis A. N. Preparation of phase 1Q fever antigen suitable for vaccine use. Appl Environ Microbiol. 1976 Jul;32(1):85–88. doi: 10.1128/aem.32.1.85-88.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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