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. 1978 Feb;19(2):562–569. doi: 10.1128/iai.19.2.562-569.1978

Protein-free and low-protein media for the cultivation of Leptospira.

R F Bey, R C Johnson
PMCID: PMC414120  PMID: 631889

Abstract

A protein-free medium composed of charcoal-detoxified Tweens (polysorbates), vitamins B12 and B1, inorganic salts, and organic buffer is described that supports the growth and subculture of pathogenic and saprophytic Leptospira. Growth was initiated from small inocula, and cell densities of 10(9) organisms per ml were attained. Antigenicity and immunogenicity of Leptospira cultivated in this medium were similar to those of cells cultivated in serum-containing media. The protein-free medium was converted to a low-protein medium by the addition of 0.1% bovine serum albumin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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