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. 1979 May;24(2):379–386. doi: 10.1128/iai.24.2.379-386.1979

Assays for Endogenous and Exogenous Lymphoid Leukosis Viruses and Chick Helper Factor with RSV(−) Cell Lines

L B Crittenden 1, D A Eagen 1, F A Gulvas 1
PMCID: PMC414312  PMID: 222685

Abstract

Japanese quail cells transformed by the envelope-defective Bryan high-titer strain of Rous sarcoma virus [R(−)Q] were used as a source of the Rous sarcoma virus genome in three kinds of assays. (i) The simplest and most sensitive assay for infectious, endogenous viruses of the chicken belonging to subgroup E involved infection of a mixture of R(−)Q cells and turkey cells with the sample and assay of supernatants of these cells for focus formation on subgroup E susceptible cells. (ii) Inactivated Sendai virus-induced fusion of R(−)Q cells with live test cells was found to be a specific method for detection of chick helper factor. Focus formation by supernatant of the fused cells on subgroup E susceptible cells was correlated with the presence of subgroup E envelope glycoprotein on the plasma membranes of test cells. Whole blood cells as well as fibroblasts could be used in this assay. (iii) A method of assay for exogenous lymphoid leukosis viruses in which mixed cultures of R(−)Q cells and C/E cells and assay of supernatants for focus formation on C/E cells was as sensitive as assays presently used for exogenous lymphoid leukosis virus. Because no infectious Rous sarcoma virus was used as part of the procedure, the assays for infectious virus described here yielded pure pseudotypes of the input virus, an advantage for determining purity and subgroup of the input virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calneck B. W., Payne L. N. Lack of correlation between Marena tumor induction and expression of endogenous avian RNA tumor virus genome. Int J Cancer. 1976 Feb 15;17(2):235–244. doi: 10.1002/ijc.2910170212. [DOI] [PubMed] [Google Scholar]
  2. Crittenden L. B., Motta J. V., Smith E. J. Genetic control of RAV-O production in chickens. Virology. 1977 Jan;76(1):90–97. doi: 10.1016/0042-6822(77)90285-9. [DOI] [PubMed] [Google Scholar]
  3. Crittenden L. B., Smith E. J., Weiss R. A., Sarma P. S. Host gene control of endogenous avian leukosis virus production. Virology. 1974 Jan;57(1):128–138. doi: 10.1016/0042-6822(74)90114-7. [DOI] [PubMed] [Google Scholar]
  4. Friis R. R. Abortive infection of Japanese quail cells with avian sarcoma viruses. Virology. 1972 Dec;50(3):701–712. doi: 10.1016/0042-6822(72)90424-2. [DOI] [PubMed] [Google Scholar]
  5. Fujita D. J., Chen Y. C., Friis R. R., Vogt P. K. RNA tumor viruses of pheasants: characterization of avian leukosis subgroups F and G. Virology. 1974 Aug;60(2):558–571. doi: 10.1016/0042-6822(74)90350-x. [DOI] [PubMed] [Google Scholar]
  6. Graf T. A plaque assay for avian RNA tumor viruses. Virology. 1972 Nov;50(2):567–578. doi: 10.1016/0042-6822(72)90408-4. [DOI] [PubMed] [Google Scholar]
  7. Halpern M. S., Bolognesi D. P., Friis R. R., Mason W. S. Expression of the Major Viral Glycoprotein of Avian Tumor Virus in Cells of chf(+) Chicken Embryos. J Virol. 1975 May;15(5):1131–1140. doi: 10.1128/jvi.15.5.1131-1140.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hanafusa H., Miyamoto T., Hanafusa T. A cell-associated factor essential for formation of an infectious form of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1970 Jun;66(2):314–321. doi: 10.1073/pnas.66.2.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanafusa T., Hanafusa H., Miyamoto T., Fleissner E. Existence and expression of tumor virus genes in chick embryo cells. Virology. 1972 Feb;47(2):475–482. doi: 10.1016/0042-6822(72)90283-8. [DOI] [PubMed] [Google Scholar]
  10. Ishizaki R., Vogt P. K. Immunological relationships among envelope antigens of avian tumor viruses. Virology. 1966 Nov;30(3):375–387. doi: 10.1016/0042-6822(66)90116-4. [DOI] [PubMed] [Google Scholar]
  11. Kao F. T. Identification of chick chromosomes in cell hybrids formed between chick erythrocytes and adenine-requiring mutants of Chinese hamster cells. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2893–2898. doi: 10.1073/pnas.70.10.2893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy H. M. A new replication-defective variant of the Bryan high-titer strain Rous sarcoma virus. Virology. 1977 Apr;77(2):705–721. doi: 10.1016/0042-6822(77)90493-7. [DOI] [PubMed] [Google Scholar]
  13. OKADA Y. Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. I. Microscopic observation of giant polynuclear cell formation. Exp Cell Res. 1962 Feb;26:98–107. doi: 10.1016/0014-4827(62)90205-7. [DOI] [PubMed] [Google Scholar]
  14. Okazaki W., Purchase H. G., Burmester B. R. Phenotypic mixing test to detect and assay avian leukosis viruses. Avian Dis. 1975 Apr-Jun;19(2):311–317. [PubMed] [Google Scholar]
  15. Rispens B. H., Long P. A., Okazaki W., Burmester B. R. The NP activation test for assay of avian leukosis-sarcoma viruses. Avian Dis. 1970 Nov;14(4):738–751. [PubMed] [Google Scholar]
  16. Robinson H. L. Inheritance and expression of chicken genes that are related to avian leukosis sarcoma virus genes. Curr Top Microbiol Immunol. 1978;83:1–36. doi: 10.1007/978-3-642-67087-9_1. [DOI] [PubMed] [Google Scholar]
  17. Robinson H. L. Intracellular restriction on the growth of induced subgroup E avian type C viruses in chicken cells. J Virol. 1976 Jun;18(3):856–866. doi: 10.1128/jvi.18.3.856-866.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Robinson H. L., Lamoreux W. F. Expression of endogenous ALV antigens and susceptibility to subgroup E ALV in three strains of chickens (endogenous avian C-type virus). Virology. 1976 Jan;69(1):50–62. doi: 10.1016/0042-6822(76)90193-8. [DOI] [PubMed] [Google Scholar]
  19. Rubin H. A VIRUS IN CHICK EMBRYOS WHICH INDUCES RESISTANCE IN VITRO TO INFECTION WITH ROUS SARCOMA VIRUS. Proc Natl Acad Sci U S A. 1960 Aug;46(8):1105–1119. doi: 10.1073/pnas.46.8.1105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SARMA P. S., TURNER H. C., HUEBNER R. J. AN AVIAN LEUCOSIS GROUP-SPECIFIC COMPLEMENT FIXATION REACTION. APPLICATION FOR THE DETECTION AND ASSAY OF NON-CYTOPATHOGENIC LEUCOSIS VIRUSES. Virology. 1964 Jul;23:313–321. doi: 10.1016/0042-6822(64)90253-3. [DOI] [PubMed] [Google Scholar]
  21. Sarma P. S., Vass W., Huebner R. J. Evidence for the in vitro transfer of defective Rous sarcoma virus genome from hamster tumor cells to chick cells. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1435–1442. doi: 10.1073/pnas.55.6.1435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smith E. J., Crittenden L. B., Brinsfield T. H., Jr Status of the endogenous avian leukosis virus in resistant cells from a producing line. Virology. 1974 Oct;61(2):594–596. doi: 10.1016/0042-6822(74)90293-1. [DOI] [PubMed] [Google Scholar]
  23. Smith E. J., Crittenden L. B., Whitson A. K. Radioimmunoassay for the envelope glycoprotein of subgroup E avian leukosis-sarcoma viruses. Virology. 1978 Feb;84(2):331–340. doi: 10.1016/0042-6822(78)90252-0. [DOI] [PubMed] [Google Scholar]
  24. Smith E. J. Preparation of antisera to group-specific antigens of avian leukosis-sarcoma viruses: an alternate approach. Avian Dis. 1977 Apr-Jun;21(2):290–299. [PubMed] [Google Scholar]
  25. Toyoshima K., Vogt P. K. Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions. Virology. 1969 Jul;38(3):414–426. doi: 10.1016/0042-6822(69)90154-8. [DOI] [PubMed] [Google Scholar]
  26. Vogt P. K., Friis R. R. An avian leukosis virus related to RSV(O): properties and evidence for helper activity. Virology. 1971 Jan;43(1):223–234. doi: 10.1016/0042-6822(71)90240-6. [DOI] [PubMed] [Google Scholar]
  27. Vogt P. K., Ishizaki R. Patterns of viral interference in the avian leukosis and sarcoma complex. Virology. 1966 Nov;30(3):368–374. doi: 10.1016/0042-6822(66)90115-2. [DOI] [PubMed] [Google Scholar]
  28. Weiss R. A., Friis R. R., Katz E., Vogt P. K. Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. Virology. 1971 Dec;46(3):920–938. doi: 10.1016/0042-6822(71)90091-2. [DOI] [PubMed] [Google Scholar]
  29. Weiss R. A., Mason W. S., Vogt P. K. Genetic recombinants and heterozygotes derived from endogenous and exogenous avian RNA tumor viruses. Virology. 1973 Apr;52(2):535–552. doi: 10.1016/0042-6822(73)90349-8. [DOI] [PubMed] [Google Scholar]
  30. Weiss R. A., Payne L. N. The heritable nature of the factor in chicken cells which acts as a helper virus for Rous sarcoma virus. Virology. 1971 Aug;45(2):508–515. doi: 10.1016/0042-6822(71)90351-5. [DOI] [PubMed] [Google Scholar]

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