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. 1979 Sep;25(3):857–862. doi: 10.1128/iai.25.3.857-862.1979

Immunization with major outer membrane proteins in experimental salmonellosis of mice.

N Kuusi, M Nurminen, H Saxen, M Valtonen, P H Mäkelä
PMCID: PMC414527  PMID: 387596

Abstract

Porin (outer membrane protein) preparations extracted from a rough (Rb2) mutant of Salmonella typhimurium proved to be good immunogens in mice and rabbits. The antibody response achieved was measured by using enzyme-linked immunosorbent assay techniques. High titers of both antiporin and antilipopolysaccharide were detected in both species. The rabbit antiserum raised against the porins and the porin preparations themselves had a highly significant protective capacity against intraperitoneal Salmonella infection of mice. Absorption of the rabbit antiporin serum with lipopolysaccharide immunosorbent did not change its protective capacity in a passive immunization experiment, suggesting that the antiporin antibody preparations were the active components.

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Selected References

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