Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Nov;26(2):775–778. doi: 10.1128/iai.26.2.775-778.1979

Isoelectric focusing of Chlamydia trachomatis.

R J Kraaipoel, A M van Duin
PMCID: PMC414682  PMID: 44706

Abstract

HeLa-229 cells and the elementary bodies of Chlamydia trachomatis had a net negative electrical surface charge at neutral pH when measured by isoelectric focusing. Inclusion-forming and non-inclusion-forming elementary bodies focused in one band at pI 4.64.

Full text

PDF
775

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker Y., Hochberg E., Zakay-Rones Z. Interaction of trachoma elementary bodies with host cells. Isr J Med Sci. 1969 Jan-Feb;5(1):121–124. [PubMed] [Google Scholar]
  2. Harrison M. J. Enhancing effect of DEAE-Dextran on inclusion counts of an ovine Chlamydia (Bedsonia) in cell culture. Aust J Exp Biol Med Sci. 1970 Apr;48(2):207–213. doi: 10.1038/icb.1970.20. [DOI] [PubMed] [Google Scholar]
  3. Hatch T. P. Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication. Infect Immun. 1975 Jul;12(1):211–220. doi: 10.1128/iai.12.1.211-220.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kuo C. C., Grayston T. Interaction of Chlamydia trachomatis organisms and HeLa 229 cells. Infect Immun. 1976 Apr;13(4):1103–1109. doi: 10.1128/iai.13.4.1103-1109.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kuo C. C., Wang S. P., Grayston J. T. Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction. Infect Immun. 1973 Jul;8(1):74–79. doi: 10.1128/iai.8.1.74-79.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kuo C., Wang S., Grayston J. T. Differentiation of TRIC and LGV organisms based on enhancement of infectivity by DEAE-dextran in cell culture. J Infect Dis. 1972 Mar;125(3):313–317. doi: 10.1093/infdis/125.3.313. [DOI] [PubMed] [Google Scholar]
  7. REEVE P., TAVERNE J. A simple method for total particle counts of trachoma and inclusion blennorrhoea viruses. Nature. 1962 Sep 1;195:923–924. doi: 10.1038/195923a0. [DOI] [PubMed] [Google Scholar]
  8. Ripa K. T., Mårdh P. A. Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells. J Clin Microbiol. 1977 Oct;6(4):328–331. doi: 10.1128/jcm.6.4.328-331.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rota T. R., Nichols R. L. Infection of cell cultures by trachoma agent: enhancement by DEAE-dextran. J Infect Dis. 1971 Oct;124(4):419–421. doi: 10.1093/infdis/124.4.419. [DOI] [PubMed] [Google Scholar]
  10. Sherbet G. V., Lakshimi M. S., Rao K. V. Characterisation of ionogenic groups and estimation of the net negative electric charge on the surface of cells using natural pH gradients. Exp Cell Res. 1972 Jan;70(1):113–123. doi: 10.1016/0014-4827(72)90188-7. [DOI] [PubMed] [Google Scholar]
  11. Wang S. P., Grayston J. T. Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. Am J Ophthalmol. 1970 Sep;70(3):367–374. doi: 10.1016/0002-9394(70)90096-6. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES