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. 1974 Jan;9(1):95–100. doi: 10.1128/iai.9.1.95-100.1974

Effect of Endotoxin on Tumor Resistance in Mice

Cynara Yang 1, Alois Nowotny 1
PMCID: PMC414771  PMID: 4587387

Abstract

As reported earlier, an intraperitoneal injection of 1 μg of endotoxin (ET) from Serratia marcescens rendered mice resistant against the nonspecific mouse ascites tumor TA3-Ha upon challenge 24 h after pretreatment with ET. Further studies were aimed at the elaboration of conditions which achieved maximal resistance. It appears that (i) a 10-μg dose of ET was approximately the optimal dose for protection; (ii) pretreatment with ET 3 to 0 days prior to tumor challenge gave best protection; and (iii) the intravenous injection of ET showed a lower protection against the tumor than intraperitoneal application. Studies on the mechanism of ET protection indicate that (i) ET does not have a direct cytotoxic effect on tumor cells; (ii) normal spleen cells exposed to ET in vitro can adoptively transfer protection against tumor; and (iii) spleen cells activated in vivo by intravenous injection of ET can adoptively transfer protection. The possible involvement of mononuclear cells is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander P., Evans R. Endotoxin and double stranded RNA render macrophages cytotoxic. Nat New Biol. 1971 Jul 21;232(29):76–78. doi: 10.1038/newbio232076a0. [DOI] [PubMed] [Google Scholar]
  2. Andersson J., Sjöberg O., Möller G. Mitogens as probes for immunocyte activation and cellular cooperation. Transplant Rev. 1972;11:131–177. doi: 10.1111/j.1600-065x.1972.tb00048.x. [DOI] [PubMed] [Google Scholar]
  3. Gery I., Krüger J., Spiesel S. Z. Stimulation of B-lymphocytes by endotoxin. Reactions of thymus-deprived mice and karyotypic analysis of dividing cells in mice bearing T 6 T 6 thymus grafts. J Immunol. 1972 Apr;108(4):1088–1091. [PubMed] [Google Scholar]
  4. Golub S., Gröschel D., Nowotny A. Factors which affect the reticuloendothelial system uptake of bacterial endotoxins. J Reticuloendothel Soc. 1968 Aug;5(4):324–339. [PubMed] [Google Scholar]
  5. Grohsman J., Nowotny A. The immune recognition of TA3 tumors, its facilitation by endotoxin, and abrogation by ascites fluid. J Immunol. 1972 Nov;109(5):1090–1095. [PubMed] [Google Scholar]
  6. Hibbs J. B., Jr Macrophage nonimmunologic recognition: target cell factors related to contact inhibition. Science. 1973 May 25;180(4088):868–870. doi: 10.1126/science.180.4088.868. [DOI] [PubMed] [Google Scholar]
  7. Hoy W. E., Nelson D. S. Studies on cytophilic antibodies. V. Alloantibodies cytophilic for mouse macrophages. Aust J Exp Biol Med Sci. 1969 Oct;47(5):525–539. doi: 10.1038/icb.1969.147. [DOI] [PubMed] [Google Scholar]
  8. Mergenhagen S. E., Snyderman R., Gewurz H., Shin H. S. Significance of complement to the mechanism of action of endotoxin. Curr Top Microbiol Immunol. 1969;50:37–77. doi: 10.1007/978-3-642-46169-9_2. [DOI] [PubMed] [Google Scholar]
  9. Parr I., Wheeler E., Alexander P. Similarities of the anti-tumour actions of endotoxin, lipid A and double-stranded RNA. Br J Cancer. 1973 May;27(5):370–389. doi: 10.1038/bjc.1973.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Peavy D. L., Shands J. W., Jr, Adler W. H., Smith R. T. Mitogenicity of bacterial endotoxins: characterization of the mitogenic principle. J Immunol. 1973 Aug;111(2):352–357. [PubMed] [Google Scholar]
  11. Perlmann P., Perlmann H., Wigzell H. Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells. Transplant Rev. 1972;13:91–114. doi: 10.1111/j.1600-065x.1972.tb00061.x. [DOI] [PubMed] [Google Scholar]
  12. Pudifin D. J., Harding B., MacLennan I. C. The differential effect of gamma irradiation on the sensitizing and effector stages of antibody dependent lymphocyte mediated cytotoxicity. Immunology. 1971 Nov;21(5):853–860. [PMC free article] [PubMed] [Google Scholar]
  13. Rosenstreich D. L., Nowotny A., Chused T., Mergenhagen S. E. In vitro transformation of mouse bone-marrow-derived (B) lymphocytes induced by the lipid component of endotoxin. Infect Immun. 1973 Sep;8(3):406–411. doi: 10.1128/iai.8.3.406-411.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tizard I. R. Macrophage cytophilic antibody in mice: mechanism of action of bacterial lipopolysaccharide on the uptake of immunoglobulins by mouse peritoneal cells. Infect Immun. 1971 Oct;4(4):402–406. doi: 10.1128/iai.4.4.402-406.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tripodi D., Hollenbeck L., Pollack W. The effect of endotoxin on the implantation of a mouse sarcoma. Int Arch Allergy Appl Immunol. 1970;37(6):575–585. doi: 10.1159/000230246. [DOI] [PubMed] [Google Scholar]

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