Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Feb;9(2):354–362. doi: 10.1128/iai.9.2.354-362.1974

Relationship of Antimicrobial Cellular Immunity to Delayed Hypersensitivity in Listeriosis

John W Osebold a, Leonard D Pearson a,1, Nicolas I Medin a,2
PMCID: PMC414809  PMID: 4205948

Abstract

The relationship of antimicrobial cellular immunity to delayed hypersensitivity (DH) was studied in mice antigenically stimulated by living Listeria monocytogenes confined to diffusion chambers in peritoneal cavities or by subcutaneous inoculation of sublethal doses of the organism. Mice showed DH reactions when tested 6 days after inoculation, and reactions were positive for at least 90 days in some mice. DH also became established when the mice were stimulated by antigens diffusing from peritoneal chambers containing viable Listeria. Mice were categorized as DH positive or DH negative if they developed more or less than a 5% increase in foot volume 24 h after the injection of Listeria antigen. Some antigenically stimulated mice did not elicit the DH reaction. Consequently, the animals were arranged as immunized groups (DH positive and DH negative) and Listeria chamber implant groups (DH positive and DH negative). When challenged with L. monocytogenes, all four groups were significantly resistant as compared with controls. Thus, the in vivo tests for immunity and DH did not show direct correlation. The results suggested that antimicrobial cellular immunity can occur as a phenomenon independent of DH. Evidence for antimicrobial cellular immunity as the principle mechanism of resistance in murine listeriosis is discussed with consideration for possible heterogeneity of function by thymus-derived lymphocytes.

Full text

PDF
358

Images in this article

357Image
on p.357
357Image
on p.357
357Image
on p.357
357Image
on p.357

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benacerraf B., Green I. Cellular hypersensitivity. Annu Rev Med. 1969;20:141–154. doi: 10.1146/annurev.me.20.020169.001041. [DOI] [PubMed] [Google Scholar]
  2. Bloom B. R. In vitro approaches to the mechanism of cell-mediated immune reactions. Adv Immunol. 1971;13:101–208. doi: 10.1016/s0065-2776(08)60184-4. [DOI] [PubMed] [Google Scholar]
  3. Dodd R. Y., Nash C. Modification of Listeria monocytogenes infection during unrelated cell-mediated immune reactions. J Reticuloendothel Soc. 1971 Dec;10(6):501–514. [PubMed] [Google Scholar]
  4. Dumonde D. C., Wolstencroft R. A., Panayi G. S., Matthew M., Morley J., Howson W. T. "Lymphokines": non-antibody mediators of cellular immunity generated by lymphocyte activation. Nature. 1969 Oct 4;224(5214):38–42. doi: 10.1038/224038a0. [DOI] [PubMed] [Google Scholar]
  5. Klun C. L., Youmans G. P. The effect of lymphocyte supernatant fluids on the intracellular growth of virulent tubercle bacilli. J Reticuloendothel Soc. 1973 Mar;13(3):263–274. [PubMed] [Google Scholar]
  6. Krahenbuhl J. L., Remington J. S. In vitro induction of nonspecific resistance in macrophages by specifically sensitized lymphocytes. Infect Immun. 1971 Oct;4(4):337–343. doi: 10.1128/iai.4.4.337-343.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lane F. C., Unanue E. R. Requirement of thymus (T) lymphocytes for resistance to listeriosis. J Exp Med. 1972 May 1;135(5):1104–1112. doi: 10.1084/jem.135.5.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MIKI K., MACKANESS G. B. THE PASSIVE TRANSFER OF ACQUIRED RESISTANCE TO LISTERIA MONOCYTOGENES. J Exp Med. 1964 Jul 1;120:93–103. doi: 10.1084/jem.120.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mackaness G. B., Blanden R. V. Cellular immunity. Prog Allergy. 1967;11:89–140. [PubMed] [Google Scholar]
  11. Mackaness G. B., Hill W. C. The effect of anti-lymphocyte globulin on cell-mediated reistance to infection. J Exp Med. 1969 May 1;129(5):993–1012. doi: 10.1084/jem.129.5.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mackaness G. B. The immunology of antituberculous immunity. Am Rev Respir Dis. 1968 Mar;97(3):337–344. doi: 10.1164/arrd.1968.97.3.337. [DOI] [PubMed] [Google Scholar]
  13. Mackaness G. B. The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969 May 1;129(5):973–992. doi: 10.1084/jem.129.5.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mackaness G. B. The monocyte in cellular immunity. Semin Hematol. 1970 Apr;7(2):172–184. [PubMed] [Google Scholar]
  15. NJOKU-OBI A. N., OSEBOLD J. W. Studies on mechanisms of immunity in listeriosis. I. Interaction of peritoneal exudate cells from sheep with Listeria monocytogenes in vitro. J Immunol. 1962 Aug;89:187–194. [PubMed] [Google Scholar]
  16. North R. J. Cellular kinetics associated with the development of acquired cellular resistance. J Exp Med. 1969 Aug 1;130(2):299–314. doi: 10.1084/jem.130.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. OSEBOLD J. W., SAWYER M. T. Immunization studies on listeriosis in mice. J Immunol. 1957 Apr;78(4):262–268. [PubMed] [Google Scholar]
  18. Osebold J. W., DiCapua R. A. Cellular immunity of mice infected with Listeria monocytogenes in diffusion chambers. J Bacteriol. 1968 Jun;95(6):2158–2164. doi: 10.1128/jb.95.6.2158-2164.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Osebold J. W., Outteridge P. M., Pearson L. D., Dicapua R. A. Cellular Responses of Mice to Diffusion Chambers I. Reactions to Intraperitoneal Diffusion Chambers Containing Listeria monocytogenes and to Bacteria-Free Chambers. Infect Immun. 1970 Aug;2(2):127–131. doi: 10.1128/iai.2.2.127-131.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Osebold J. W., Outteridge P. M., Pearson L. D. Mutation of Listeria monocytogenes After Prolonged In Vivo Survival in Diffusion Chambers. Infect Immun. 1970 Feb;1(2):209–211. doi: 10.1128/iai.1.2.209-211.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Outteridge P. M., Osebold J. W., Zee Y. C. Activity of macrophage and neutrophil cellular fractions from normal and immune sheep against Listeria monocytogenes. Infect Immun. 1972 May;5(5):814–825. doi: 10.1128/iai.5.5.814-825.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pearson L. D., Osebold J. W. Effects of antithymocyte and antimacrophage sera on the survival of mice infected with Listeria monocytogenes. Infect Immun. 1973 Mar;7(3):479–486. doi: 10.1128/iai.7.3.479-486.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pearson L. D., Osebold J. W. Effects of antithymocyte sera and antimacrophage sera on cell-mediated immune reactions in Listeria-infected mice. Infect Immun. 1974 Jan;9(1):127–133. doi: 10.1128/iai.9.1.127-133.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pearson L. D., Osebold J. W., Wagner P. C. A device for measuring the volume of footpad swelling from delayed hypersensitivity reactions in mice. Lab Anim Sci. 1971 Aug;21(4):591–593. [PubMed] [Google Scholar]
  25. Simon H. B., Sheagren J. N. Migration inhibitory factor and macrophage bactericidal function. Infect Immun. 1972 Aug;6(2):101–103. doi: 10.1128/iai.6.2.101-103.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Spencer H. D., Cozad G. C. Role of delayed hypersensitivity in blastomycosis of mice. Infect Immun. 1973 Mar;7(3):329–334. doi: 10.1128/iai.7.3.329-334.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. TALMAGE D. W. Immunological specificity, unique combinations of selected natural globulins provide an alternative to the classical concept. Science. 1959 Jun 19;129(3364):1643–1648. doi: 10.1126/science.129.3364.1643. [DOI] [PubMed] [Google Scholar]
  28. Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Adv Immunol. 1972;15:95–165. doi: 10.1016/s0065-2776(08)60684-7. [DOI] [PubMed] [Google Scholar]
  29. Youmans G. P., Youmans A. S. Recent studies on acquired immunity in tuberculosis. Curr Top Microbiol Immunol. 1969;48:129–178. doi: 10.1007/978-3-642-46163-7_6. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES