Abstract
Deoxyribonucleic acid (DNA) base composition, as measured by guanine plus cytosine (G + C) content, was determined in 17 strains of Sporothrix schenckii and Ceratocystis species. The average G + C content for S. schenckii was 54.7 ± 0.2 mol%. No strain of Ceratocystis, with the exception of C. minor, gave values for G + C content similar to those obtained for S. schenckii. All DNA samples from strains of S. schenckii cross-hybridized with high percentages of relative binding. DNA samples from C. stenoceras and C. pilifera showed low degrees of homology with S. schenckii DNA. The DNA from C. minor hybridized with 75% binding with that from a strain of S. schenckii, as compared with the homologous reaction.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andrieu S., Biguet J., Massamba S. Etude immunologique comparee de Sporothrix schenckii et des souches saprophytes voisines. Sabouraudia. 1971 Nov;9(3):206–209. [PubMed] [Google Scholar]
- Bernardi G., Carnevali F., Nicolaieff A., Piperno G., Tecce G. Separation and characterization of a satellite DNA from a yeast cytoplasmic "petite" mutant. J Mol Biol. 1968 Nov 14;37(3):493–505. doi: 10.1016/0022-2836(68)90117-4. [DOI] [PubMed] [Google Scholar]
- Commerford S. L. Iodination of nucleic acids in vitro. Biochemistry. 1971 May 25;10(11):1993–2000. doi: 10.1021/bi00787a005. [DOI] [PubMed] [Google Scholar]
- Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
- Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
- Johnson J. L., Ordal E. J. Deoxyribonucleic acid homology in bacterial taxonomy: effect of incubation temperature on reaction specificity. J Bacteriol. 1968 Mar;95(3):893–900. doi: 10.1128/jb.95.3.893-900.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lloyd K. O., Bitoon M. A. Isolation and purification of a peptido-rhamnomannan from the yeast form of Sporothrix schenckii. Structural and immunochemical studies. J Immunol. 1971 Sep;107(3):663–671. [PubMed] [Google Scholar]
- Mariat F. Adaptation de Ceratocystis à la vie parasitaire chez l'animal--étude de l'aquisition d'un pouvoir pathogene comparable à celui de Sporothrix schenckii. Sabouraudia. 1971 Nov;9(3):191–205. [PubMed] [Google Scholar]
- Mariat F., Escudié A., Gaxotte P. Isolement de souches de Ceratocystis sp. à forme conidienne Sporotrichum, de cuirs chevelus humains et de poils de rats. Comparaison avec l'espèce pathogène Sporotrichum schenckii. C R Acad Sci Hebd Seances Acad Sci D. 1968 Sep 2;267(10):974–976. [PubMed] [Google Scholar]
- Nicot J., Mariat F. Caractères morphologique et position systématique de Sporothrix schenckii, agent de la sporotrichose humaine. Mycopathol Mycol Appl. 1973 Jan 31;49(1):53–65. doi: 10.1007/BF02057447. [DOI] [PubMed] [Google Scholar]
- SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
- Storck R., Alexopoulos C. J. Deoxyribonucleic acid of fungi. Bacteriol Rev. 1970 Jun;34(2):126–154. doi: 10.1128/br.34.2.126-154.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor J. J. A comparison of some Ceratocystis species with Sporothrix schenckii. Mycopathol Mycol Appl. 1970 Dec 29;42(3):233–240. doi: 10.1007/BF02051951. [DOI] [PubMed] [Google Scholar]
- Travassos L. R., Gorin P. A., Lloyd K. O. Comparison of the rhamnomannans from the human pathogen Sporothrix schenckii with those from the Ceratocystis species. Infect Immun. 1973 Nov;8(5):685–693. doi: 10.1128/iai.8.5.685-693.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Travassos L. R., Gorin P. A., Lloyd K. O. Discrimination between Sporothrix schenckii and Ceratocystis stenoceras rhamnomannans by proton and carbon-13 magnetic resonance spectroscopy. Infect Immun. 1974 Apr;9(4):674–680. doi: 10.1128/iai.9.4.674-680.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]