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. 1974 Jul;10(1):270–276. doi: 10.1128/iai.10.1.270-276.1974

Ultrastructure of Hamster Kidney Cell Culture Infected with Herpesvirus

Farouk Hamdy a,1, Stanley C Holt a, Martin Sevoian a
PMCID: PMC414988  PMID: 4842129

Abstract

Hamster kidney cell culture was found susceptible to infection and replication of herpesvirus of turkey (HVT) as evidenced by cytopathic effect, induction of HVT-specific, complement-fixing antigen, and demonstration of virus particles by electron microscopy. Viral structures at different developmental stages were observed. Several infected cells showed a lamellar inclusion body possibly continuous with the endoplasmic reticulum. The function of these lamellar bodies is not known.

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Selected References

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  1. Bedigian H. G., Sevoian M. Susceptibility of mammalian (hamster) cell culture to infection with herpesvirus of turkeys. Appl Microbiol. 1972 Aug;24(2):275–280. doi: 10.1128/am.24.2.275-280.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Darlington R. W., Moss L. H., 3rd Herpesvirus envelopment. J Virol. 1968 Jan;2(1):48–55. doi: 10.1128/jvi.2.1.48-55.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HUXLEY H. E., ZUBAY G. Preferential staining of nucleic acid-containing structures for electron microscopy. J Biophys Biochem Cytol. 1961 Nov;11:273–296. doi: 10.1083/jcb.11.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hamdy F., Sevoian M. Complement fixation reaction for the diagnosis of type II avian (Marek's) leukosis. Appl Microbiol. 1970 Sep;20(3):356–361. doi: 10.1128/am.20.3.356-361.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harris H., Watkins J. F., Ford C. E., Schoefl G. I. Artificial heterokaryons of animal cells from different species. J Cell Sci. 1966 Mar;1(1):1–30. doi: 10.1242/jcs.1.1.1. [DOI] [PubMed] [Google Scholar]
  6. Kawamura H., King D. J., Jr, Anderson D. P. A herpesvirus isolated from kidney cell culture of normal turkeys. Avian Dis. 1969 Nov;13(4):853–863. [PubMed] [Google Scholar]
  7. King N. W., Daniel M. D., Barahona H. H., Meléndez L. V. Viruses from South American monkeys: ultrastructural studies. J Natl Cancer Inst. 1972 Jul;49(1):273–290. [PubMed] [Google Scholar]
  8. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Meiselman N., Kohn A., Danon D. Electron microscopic study of penetration of Newcastle disease virus into cells leading to formation of polykaryocytes. J Cell Sci. 1967 Mar;2(1):71–76. doi: 10.1242/jcs.2.1.71. [DOI] [PubMed] [Google Scholar]
  10. Mikami T., Bankowski R. A. The effect of DMSO and cultural behavior of two herpesviruses associated with Marek's disease. Avian Dis. 1971 Apr-Jun;15(2):242–253. [PubMed] [Google Scholar]
  11. Nazerian K., Lee L. F., Witter R. L., Burmester B. R. Ultrastructural studies of a herpesvirus of turkeys antigenically related to Marek's disease virus. Virology. 1971 Feb;43(2):442–452. doi: 10.1016/0042-6822(71)90316-3. [DOI] [PubMed] [Google Scholar]
  12. Nii S., Morgan C., Rose H. M. Electron microscopy of herpes simplex virus. II. Sequence of development. J Virol. 1968 May;2(5):517–536. doi: 10.1128/jvi.2.5.517-536.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. ROIZMAN B. Polykaryocytosis induced by viruses. Proc Natl Acad Sci U S A. 1962 Feb;48:228–234. doi: 10.1073/pnas.48.2.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROIZMAN B. Polykaryocytosis. Cold Spring Harb Symp Quant Biol. 1962;27:327–342. doi: 10.1101/sqb.1962.027.001.031. [DOI] [PubMed] [Google Scholar]
  16. Schwartz J., Roizman B. Similarities and Differences in the Development of Laboratory Strains and Freshly Isolated Strains of Herpes Simplex Virus in HEp-2 Cells: Electron Microscopy. J Virol. 1969 Dec;4(6):879–889. doi: 10.1128/jvi.4.6.879-889.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Simoni P., Biagini G., Maraldi N. M., Barbieri M., Bersani F. Ribosome crystallization in normal and in Marek's disease affected chicken. Exp Cell Res. 1973 Apr;78(2):433–440. doi: 10.1016/0014-4827(73)90088-8. [DOI] [PubMed] [Google Scholar]
  18. WATSON D. H., WILDY P., RUSSELL W. C. QUANTITATIVE ELECTRON MICROSCOPY STUDIES ON THE GROWTH OF HERPES VIRUS USING THE TECHNIQUES OF NEGATIVE STAINING AND ULTRAMICROTOMY. Virology. 1964 Dec;24:523–538. doi: 10.1016/0042-6822(64)90204-1. [DOI] [PubMed] [Google Scholar]
  19. Witter R. L., Nazerian K., Purchase H. G., Burgoyne G. H. Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek's disease virus. Am J Vet Res. 1970 Mar;31(3):525–538. [PubMed] [Google Scholar]

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