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. 1975 Jun;11(6):1195–1202. doi: 10.1128/iai.11.6.1195-1202.1975

Immunogenicity of Ribosomal Vaccines Isolated from Group A, Type 14 Streptococcus pyogenes

W O Schalla a,1, W Johnson a
PMCID: PMC415199  PMID: 806521

Abstract

A ribosomal preparation isolated from group A, type 14 Streptococcus pyogenes protected mice against a challenge of 1,000 mean lethal doses. Optimal immunization consisted of subcutaneous administration of 326 μg of protein with Freund incomplete adjuvant. Animals immunized with higher or lower antigen concentrations showed decreased survival. Freund incomplete adjuvant was necessary to insure optimum immunization. Immunochemical studies showed no M protein or C carbohydrate present in the ribosomal preparation. Mice optimally immunized with the type 14 ribosomal preparation were protected against heterologous challenge with M types 2, 5, 8, and 12. Protein-free ribonucleic acid extracts isolated from the type 14 ribosomal preparation did not protect mice against homologous challenge. But ribonucleic acid-free protein extracts showed 100% protection in mice after homologous challenge.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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