Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1975 Jun;11(6):1278–1283. doi: 10.1128/iai.11.6.1278-1283.1975

Effect of Trace Metals on Phagocytosis by Alveolar Macrophages

Judith A Graham 1, Donald E Gardner 1, Michael D Waters 1, David L Coffin 1
PMCID: PMC415211  PMID: 49299

Abstract

Experiments were performed to measure the effect of trace metals on a vital function of the alveolar macrophage (AM), phagocytosis. Since certain trace metals were found to reduce the viability of AMs, a technique was developed to permit examination of live cells only for phagocytosis. Evidence is presented that Ni2+ selectively altered the phagocytic activity of AMs at concentrations lower than those which caused cell death. It is further shown that a level of VO3− that caused extensive lysis and death did not reduce phagocytosis in surviving cells. The effects of Cd2+, Cr3+, and Mn2+ on AMs were also examined.

Full text

PDF
1280

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENNETT B., OLD L. J., BOYSE E. A. THE PHAGOCYTOSIS OF TUMOR CELLS IN VITRO. Transplantation. 1964 Mar;2:183–202. doi: 10.1097/00007890-196403000-00003. [DOI] [PubMed] [Google Scholar]
  2. Coffin D. L., Gardner D. E., Holzman R. S., Wolock F. J. Influence of ozone on pulmonary cells. Arch Environ Health. 1968 May;16(5):633–636. doi: 10.1080/00039896.1968.10665119. [DOI] [PubMed] [Google Scholar]
  3. Coffin D. L., Gardner D. E. Interaction of biological agents and chemical air pollutants. Ann Occup Hyg. 1972 Nov;15(2):219–235. doi: 10.1093/annhyg/15.2-4.219. [DOI] [PubMed] [Google Scholar]
  4. DeRenzis F. A., Schechtman A. Staining by neutral red and trypan blue in sequence for assaying vital and nonvital cultured cells. Stain Technol. 1973 May;48(3):135–136. doi: 10.3109/10520297309116602. [DOI] [PubMed] [Google Scholar]
  5. Gardner D. E., Graham J. A., Miller F. J., Illing J. W., Coffin D. L. Technique for differentiating particles that are cell-associated or ingested by macrophages. Appl Microbiol. 1973 Mar;25(3):471–475. doi: 10.1128/am.25.3.471-475.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gardner D. E., Holzman R. S., Coffin D. L. Effects of nitrogen dioxide on pulmonary cell population. J Bacteriol. 1969 Jun;98(3):1041–1043. doi: 10.1128/jb.98.3.1041-1043.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green G. M. Pulmonary clearance of infectious agents. Annu Rev Med. 1968;19:315–336. doi: 10.1146/annurev.me.19.020168.001531. [DOI] [PubMed] [Google Scholar]
  8. Green G. M. The response of the alveolar macrophage system to host and environmental changes. Arch Environ Health. 1969 Apr;18(4):548–550. doi: 10.1080/00039896.1969.10665451. [DOI] [PubMed] [Google Scholar]
  9. HANKS J. H., WALLACE J. H. Determination of cell viability. Proc Soc Exp Biol Med. 1958 May;98(1):188–192. doi: 10.3181/00379727-98-23985. [DOI] [PubMed] [Google Scholar]
  10. Lee R. E., Jr, Lehmden D. J. Trace metal pollution in the environment. J Air Pollut Control Assoc. 1973 Oct;23(10):853–857. doi: 10.1080/00022470.1973.10469854. [DOI] [PubMed] [Google Scholar]
  11. Mustafa M. G., Cross C. E., Munn R. J., Hardie J. A. Effects of divalent metal ions on alveolar macrophage membrane adenosine triphosphatase activity. J Lab Clin Med. 1971 Apr;77(4):563–571. [PubMed] [Google Scholar]
  12. Roser B. The distribution of intravenously injected Kupffer cellsin the mouse. J Reticuloendothel Soc. 1968 Oct;5(5):455–471. [PubMed] [Google Scholar]
  13. Schroeder H. A. A sensible look at air pollution by metals. Arch Environ Health. 1970 Dec;21(6):798–806. doi: 10.1080/00039896.1970.10667334. [DOI] [PubMed] [Google Scholar]
  14. Sunderman F. W., Jr The current status of nickel carcinogenesis. Ann Clin Lab Sci. 1973 May-Jun;3(3):156–180. [PubMed] [Google Scholar]
  15. Waters M. D., Gardner D. E., Aranyi C., Coffin D. L. Metal toxicity for rabbit alveolar macrophages in vitro. Environ Res. 1975 Feb;9(1):32–47. doi: 10.1016/0013-9351(75)90047-x. [DOI] [PubMed] [Google Scholar]
  16. Waters M. D., Gardner D. E., Coffin D. L. Cytotoxic effects of vanadium on rabbit alveolar macrophages in vitro. Toxicol Appl Pharmacol. 1974 May;28(2):253–263. doi: 10.1016/0041-008x(74)90012-x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES