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. 1975 Sep;12(3):614–620. doi: 10.1128/iai.12.3.614-620.1975

Growth of measles virus in cultures of rat glioma cells.

K Nakamura, M Homma, N Ishida
PMCID: PMC415331  PMID: 809359

Abstract

The interaction of measles virus with RG-6 cells derived from rat glioma was investigated. When a culture of RG-6 cells was infected with measles virus, the synthesis of viral antigens was detected in very few cells, at most 5%. The apparent resistance to measles virus infection was also repeatedly found in all of the subclonal cells derived form RG-6 cells. Although all of the virus-synthesizing cells had the ability to form plaques on Vero cells, they produced only a reduced amount of infectious virus, i.e., 0.1 plaque-forming units per cell. These results imply the existence of some mechanism that regulates growth of measles virus in cultures of RG-6 cells. The transmission of genetic material of measles virus from infected RG-6 cells to Vero cells was not inhibited in the presence of antiviral serum. This fact may provide a basis for interpretation of the persistence of virus, in the presence of antibody, in patients with subacute sclerosing panencephalitis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbanti-Brodano G., Oyanagi S., Katz M., Koprowski H. Presence of 2 different viral agents in brain cells of patients with subacute sclerosing panencephalitis. Proc Soc Exp Biol Med. 1970 May;134(1):230–236. doi: 10.3181/00379727-134-34765. [DOI] [PubMed] [Google Scholar]
  2. Bather R., Furesz J., Fanok A. G., Gill S. D., Yarosh W. Long term infection of diploid African green monkey brain cells by Schwarz measles vaccine virus. J Gen Virol. 1973 Sep;20(3):401–405. doi: 10.1099/0022-1317-20-3-401. [DOI] [PubMed] [Google Scholar]
  3. Benda P., Lightbody J., Sato G., Levine L., Sweet W. Differentiated rat glial cell strain in tissue culture. Science. 1968 Jul 26;161(3839):370–371. doi: 10.1126/science.161.3839.370. [DOI] [PubMed] [Google Scholar]
  4. Burnstein T., Swango L. J., Byington D. P. Non-specific brain inhibitor against measles virus. Arch Gesamte Virusforsch. 1971;34(4):396–399. doi: 10.1007/BF01242989. [DOI] [PubMed] [Google Scholar]
  5. Connolly J. H., Allen I. V., Hurwitz L. J., Millar J. H. Measles-virus antibody and antigen in subacute sclerosing panencephalitis. Lancet. 1967 Mar 11;1(7489):542–544. doi: 10.1016/s0140-6736(67)92117-4. [DOI] [PubMed] [Google Scholar]
  6. Dayan A. D., Gostling J. V., Greaves J. L., Stevens D. W., Woodhouse M. A. Evidence of a pseudomyxovirus in the brain in subacute sclerosing leucoencephalitis. Lancet. 1967 May 6;1(7497):980–981. doi: 10.1016/s0140-6736(67)92360-4. [DOI] [PubMed] [Google Scholar]
  7. Horta-Barbosa L., Fuccillo D. A., London W. T., Jabbour J. T., Zeman W., Sever J. L. Isolation of measles virus from brain cell cultures of two patients with subacute sclerosing panencephalitis. Proc Soc Exp Biol Med. 1969 Oct;132(1):272–277. doi: 10.3181/00379727-132-34196. [DOI] [PubMed] [Google Scholar]
  8. Horta-Barbosa L., Fuccillo D. A., Sever J. L., Zeman W. Subacute sclerosing panencephalitis: isolation of measles virus from a brain biopsy. Nature. 1969 Mar 8;221(5184):974–974. doi: 10.1038/221974a0. [DOI] [PubMed] [Google Scholar]
  9. Knight P., Duff R., Rapp F. Latency of human measles virus in hamster cells. J Virol. 1972 Nov;10(5):995–1001. doi: 10.1128/jvi.10.5.995-1001.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Payne F. E., Baublis J. V., Itabashi H. H. Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. N Engl J Med. 1969 Sep 11;281(11):585–589. doi: 10.1056/NEJM196909112811103. [DOI] [PubMed] [Google Scholar]
  11. Raine C. S., Feldman L. A., Sheppard R. D., Bornstein M. B. Ultrastructural study of long-term measles infection in cultures of hamster dorsal-root ganglion. J Virol. 1971 Sep;8(3):318–329. doi: 10.1128/jvi.8.3.318-329.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tellez-Negal I., Harter D. H. Subacute sclerosing leukoencephalitis: ultrastructure of intranuclear and intracytoplasmic inclusions. Science. 1966 Nov 18;154(3751):899–901. doi: 10.1126/science.154.3751.899. [DOI] [PubMed] [Google Scholar]
  13. Webb H. E., Illavia S. J., Laurence G. D. Measles-vaccine viruses in tissue-culture of non-neuronal cells of human fetal brain. Lancet. 1971 Jul 3;2(7714):4–5. doi: 10.1016/s0140-6736(71)90002-x. [DOI] [PubMed] [Google Scholar]

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