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. 1995 Jul 3;92(14):6557–6561. doi: 10.1073/pnas.92.14.6557

Functional domain analysis of glass, a zinc-finger-containing transcription factor in Drosophila.

E M O'Neill 1, M C Ellis 1, G M Rubin 1, R Tjian 1
PMCID: PMC41557  PMID: 7604032

Abstract

The glass gene is required for proper photo-receptor differentiation during development of the Drosophila eye glass codes for a DNA-binding protein containing five zinc fingers that we show is a transcriptional activator. A comparison of the sequences of the glass genes from two species of Drosophila and a detailed functional domain analysis of the Drosophila melanogaster glass gene reveal that both the DNA-binding domain and the transcriptional-activation domain are highly conserved between the two species. Analysis of the DNA-binding domain of glass indicates that the three carboxyl-terminal zinc fingers alone are necessary and sufficient for DNA binding. We also show that a deletion mutant of glass containing only the DNA-binding domain can behave in a dominant-negative manner both in vivo and in a cell culture assay that measures transcriptional activation.

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Selected References

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