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. 1970 Mar;1(3):226–231. doi: 10.1128/iai.1.3.226-231.1970

Relationship of Serogroups of Neisseria meningitidis I. Microagglutination, Gel Diffusion, and Slide Agglutination Studies of Meningococcal Antisera Before and After Absorption with RAS-10 Strain of Meningococci

Leonard F Devine 1, Cline R Hagerman 1
PMCID: PMC415885  PMID: 16557721

Abstract

Microagglutination tests were used to show the relationship of a nongroupable strain of Neisseria meningitidis (RAS-10) to other serological groups. RAS-10 antiserum has been prepared and studied for the first time. Antibodies to the RAS-10 strain were shown to be present in many grouping antisera obtained from different sources. These antibodies were absorbed from antisera to heterologous sero-groups with the RAS-10 strain. This procedure was shown to make antisera more specific by eliminating serological cross-reactions and false grouping of RAS-10 strains. Antisera before and after absorption with RAS-10 cells were studied by using double diffusion in gels. An antigen-antibody precipitation line for the RAS-10 meningococci was shown to be removed by this procedure. Antiserum to group 29E meningococci was absorbed with group Z cells, and precipitation lines for Z cells were removed. Group 29E antiserum agglutinated group 29E and group Z cells in the slide agglutination test but was specific for group 29E cells in this test after absorption with group Z cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BJORKLUND B. Specific inhibition of precipitation as an aid in antigen analysis with gel diffusion method. Proc Soc Exp Biol Med. 1952 Feb;79(2):319–324. doi: 10.3181/00379727-79-19364. [DOI] [PubMed] [Google Scholar]
  2. BRANHAM S. E. Serological relationships among meningococci. Bacteriol Rev. 1953 Sep;17(3):175–188. doi: 10.1128/br.17.3.175-188.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beno D. W., Devine L. F., Larson G. L. Identification of Neisseria meningitidis carbohydrate fermentation patterns in Mueller-Hinton broth. J Bacteriol. 1968 Aug;96(2):563–563. doi: 10.1128/jb.96.2.563-.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Evans J. R., Artenstein M. S., Hunter D. H. Prevalence of meningococcal serogroups and description of three new groups. Am J Epidemiol. 1968 May;87(3):643–646. doi: 10.1093/oxfordjournals.aje.a120854. [DOI] [PubMed] [Google Scholar]
  5. Goldschneider I., Gotschlich E. C., Artenstein M. S. Human immunity to the meningococcus. II. Development of natural immunity. J Exp Med. 1969 Jun 1;129(6):1327–1348. doi: 10.1084/jem.129.6.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hollis D. G., Wiggins G. L., Schubert J. H. Serological studies of ungroupable Neisseria meningitidis. J Bacteriol. 1968 Jan;95(1):1–4. doi: 10.1128/jb.95.1.1-4.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JYSSUM K. Serologic relationship among meningococcal strains in Norway. J Immunol. 1956 Jun;76(6):433–440. [PubMed] [Google Scholar]
  8. KAUFFMANN F. A new antigen of Salmonella paratyphi B and Salmonella typhi murium. Acta Pathol Microbiol Scand. 1956;39(4):299–304. doi: 10.1111/j.1699-0463.1956.tb03405.x. [DOI] [PubMed] [Google Scholar]
  9. Kingsbury D. T. Deoxyribonucleic acid homologies among species of the genus Neisseria. J Bacteriol. 1967 Oct;94(4):870–874. doi: 10.1128/jb.94.4.870-874.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NAIDE Y., NIKAIDO H., MAEKELAE P. H., WILKINSON R. G., STOCKER B. A. SEMIROUGH STRAINS OF SALMONELLA. Proc Natl Acad Sci U S A. 1965 Jan;53:147–153. doi: 10.1073/pnas.53.1.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SLATERUS K. W., RUYS A. C., SIEBERG I. G. TYPES OF MENINGOCOCCI ISOLATED FROM CARRIERS AND PATIENTS IN A NON-EPIDEMIC PERIOD IN THE NETHERLANDS. Antonie Van Leeuwenhoek. 1963;29:265–271. doi: 10.1007/BF02046068. [DOI] [PubMed] [Google Scholar]
  13. SLATERUS K. W. Serological typing of meningococci by means of micro-precipitation. Antonie Van Leeuwenhoek. 1961;27:305–315. doi: 10.1007/BF02538460. [DOI] [PubMed] [Google Scholar]
  14. Vedros N. A., Hill P. R. Microagglutination technique for Neisseria meningitidis. J Bacteriol. 1966 Feb;91(2):900–901. doi: 10.1128/jb.91.2.900-901.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vedros N. A., Ng J., Culver G. A new serological group (E) of Neisseria meningitidis. J Bacteriol. 1968 Apr;95(4):1300–1304. doi: 10.1128/jb.95.4.1300-1304.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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