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. 1970 Mar;1(3):293–299. doi: 10.1128/iai.1.3.293-299.1970

Bacteriocin Typing of Vibrio cholerae

A N Chakrabarty 1, Sati Adhya 1, Jayantisri Basu 1, Sujata G Dastidar 1
PMCID: PMC415895  PMID: 16557731

Abstract

Bacteriocins of Vibrio cholerae have been demonstrated against enterobacterial and vibrio indicator organisms by conventional techniques. Abundant bacteriocin production took place on casein hydrolysate-yeast extract, tryptic soy, digest broth, proteose-peptone, and neopeptone agars. Essential factors were a citrate-phosphate buffer concentration of 0.5 to 0.7%, at pH 7.5 to 7.6, and cold shock. Thermal treatment of indicator organisms at 45 C for 12 min increased the percentage of typable strains. The bacteriocins of V. cholerae appeared to be powerful diffusible bactericidal agents. By using 8 indicator strains, 11 bacteriocin types have been recognized among 425 strains, of which 87% are typable at present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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