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. 1970 Dec;2(6):742–749. doi: 10.1128/iai.2.6.742-749.1970

Failure of Immune Sera to Enhance Significantly Phagocytosis of Staphylococus aureus: Nonspecific Adsorption of Phagocytosis-Promoting Factors

Mehdi Shayegani 1,2,3
PMCID: PMC416085  PMID: 16557910

Abstract

Serum from rabbits immunized with either heat-killed or live nonencapsulated Staphylococcus aureus failed further to enhance phagocytosis and intracellular killing of the homologous organism by either normal rabbit polymorphonuclear leukocytes or monocytes, when compared with normal rabbit serum. These immune sera did, however, show an increase in agglutinating and precipitating antibody level. Adsorption of normal human serum with some gram-positive and gram-negative bacteria, yeast, and some inert particles significantly reduced the phagocytosis-promoting factors of the serum. It would seem, then, that nonencapsulated S. aureus differs from other pathogenic bacteria in that the humoral antibacterial factors promoting its phagocytosis and intracellular killing are not significantly enhanced by infection or immunization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHEN J. O., NEWTON W. L., CHERRY W. B., UPDYKE E. L. NORMALLY OCCURRING STAPHYLOCOCCAL ANTIBODIES IN GERMFREE MICE. J Immunol. 1963 Mar;90:358–367. [PubMed] [Google Scholar]
  2. Daugharty H., Martin R. R., White A. Antibodies against staphylococcal teichoic acids and type-specific antigens in man. J Immunol. 1967 Jun;98(6):1123–1129. [PubMed] [Google Scholar]
  3. HAUKENES G., OEDING P. On two new antigens in Staphylococcus aureus. Acta Pathol Microbiol Scand. 1960;49:237–248. doi: 10.1111/j.1699-0463.1960.tb01135.x. [DOI] [PubMed] [Google Scholar]
  4. HIRSCH J. G., STRAUSS B. STUDIES ON HEAT-LABILE OPSONIN IN RABBIT SERUM. J Immunol. 1964 Jan;92:145–154. [PubMed] [Google Scholar]
  5. KAPRAL F. A., SHAYEGANI M. G. Intracellular survival of staphylococci. J Exp Med. 1959 Jul 1;110(1):123–138. doi: 10.1084/jem.110.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Koenig M. G., Melly M. A., Goodman J. S., Rogers D. E. Serum factors and the reticuloendothelial uptake of Staphylococcus aureus. I. The role of whole serum. J Immunol. 1968 Mar;100(3):516–524. [PubMed] [Google Scholar]
  7. LANGE K., GRAIG F., OBERMAN J., SLOBODY L., OGUR G., LoCASTO F. Changes in serum complement during the course and treatment of glomerulonephritis. AMA Arch Intern Med. 1951 Oct;88(4):433–445. doi: 10.1001/archinte.1951.03810100017002. [DOI] [PubMed] [Google Scholar]
  8. LENHART N. A., MUDD S., YOSHIDA A., LI I. W. THE COMMON PROTEIN AGGLUTINOGEN OF STAPHYLOCOCCUS AUREUS. I. DISTRIBUTION IN INTERNATIONAL SEROTYPES AND CORRESPONDING ANTIBODY IN HUMAN POPULATIONS. J Immunol. 1963 Dec;91:771–776. [PubMed] [Google Scholar]
  9. LI I. W., MUDD S., KAPRAL F. A. DISSOCIATION OF PHAGOCYTOSIS AND INTRACELLULAR KILLING OF STAPHYLOCOCCUS AUREUS BY HUMAN BLOOD LEUKOCYTES. J Immunol. 1963 May;90:804–809. [PubMed] [Google Scholar]
  10. LI I. W., MUDD S. THE HEAT-LABILE SERUM FACTOR ASSOCIATED WITH INTRACELLULAR KILLING OF STAPHYLOCOCCUS AUREUS. J Immunol. 1965 Jun;94:852–857. [PubMed] [Google Scholar]
  11. Laxdal T., Messner R. P., Williams R. C., Jr, Quie P. G. Opsonic, agglutinating, and complement-fixing antibodies in patients with subacute bacterial endocarditis. J Lab Clin Med. 1968 Apr;71(4):638–653. [PubMed] [Google Scholar]
  12. Mudd S., Gladstone G. P., Lenhart N. A. The antigenicity in man of staphlylococcal leucocidin toxoid, with notes on therapeutic immunization in chronic osteomyelits. Br J Exp Pathol. 1965 Aug;46(4):455–472. [PMC free article] [PubMed] [Google Scholar]
  13. NATHENSON S. G., STROMINGER J. L. Enzymatic synthesis and immunochemistry of N-acetylglucosaminylribitol linkages in the teichoic acids of Staphylococcus aureus stains. J Biol Chem. 1962 Dec;237:3839–3841. [PubMed] [Google Scholar]
  14. SHAYEGANI M. G., KAPRAL F. A., MUDD S. PHAGOCYTOSIS AND INTRACELLULAR KILLING OF STAPHYLOCOCCUS AUREUS BY HUMAN AND RABBIT BLOOD LEUKOCYTES. J Immunol. 1964 Jul;93:88–93. [PubMed] [Google Scholar]
  15. SHAYEGANI M. G., KAPRAL F. A. The immediate fate of staphylococci after phagocytosis. J Gen Microbiol. 1962 Dec;29:625–636. doi: 10.1099/00221287-29-4-625. [DOI] [PubMed] [Google Scholar]
  16. Shayegani M. G. Action of lysates of leukocytic granules on staphylococcal cells walls. J Infect Dis. 1968 Oct;118(4):402–410. doi: 10.1093/infdis/118.4.402. [DOI] [PubMed] [Google Scholar]
  17. Shayegani M. G., Mudd S. Role of serum in the intracellular killing of staphylococci in rabbit monocytes. J Bacteriol. 1966 Apr;91(4):1393–1398. doi: 10.1128/jb.91.4.1393-1398.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smith M. R., Wood W. B., Jr Heat labile opsonins to pneumococcus. I. Participation of complement. J Exp Med. 1969 Dec 1;130(6):1209–1227. doi: 10.1084/jem.130.6.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. THORBECKE G. J., BENACERRAF B. Some histological and functional aspects of lymphoid tissue in germfree animals. II. Studies on phagocytosis in vivo. Ann N Y Acad Sci. 1959 May 8;78:247–253. doi: 10.1111/j.1749-6632.1959.tb53107.x. [DOI] [PubMed] [Google Scholar]
  20. Williams R. C., Jr, Dossett J. H., Quie P. G. Comparative studies of immunoglobulin opsonins in osteomyelitis and other established infections. Immunology. 1969 Aug;17(2):249–265. [PMC free article] [PubMed] [Google Scholar]
  21. Winston S. H., Berry L. J. Antibacterial immunity induced by ribosomal vaccines. J Reticuloendothel Soc. 1970 Jul;8(1):13–24. [PubMed] [Google Scholar]
  22. Yotis W. W., Ortiz J. S. A comparison of rat and rabbit sera bactericidins active against staphylococci. Can J Microbiol. 1967 Jul;13(7):885–894. doi: 10.1139/m67-116. [DOI] [PubMed] [Google Scholar]

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