Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1970 Dec;2(6):750–756. doi: 10.1128/iai.2.6.750-756.1970

Cell Wall Component Which Affects the Ability of Serum to Promote Phagocytosis and Killing of Staphylococcus aureus

Mehdi Shayegani a, Kazuhito Hisatsune b,1, Stuart Mudd c
PMCID: PMC416086  PMID: 16557911

Abstract

The role of various cell wall components of nonencapsulated Staphylococcus aureus in phagocytosis and killing was studied. Adsorption of human serum with S. aureus cell walls, or trypsin-digested cell walls, reduced the rate of phagocytosis and killing of S. aureus by human-blood leukocytes when compared to unadsorbed serum. When the various components of the cell walls were used for adsorption, only the intact mucopeptide was found to remove these factors. Products of digestion of this mucopeptide with several enzymes were found to be inactive when used for adsorption of human serum. Other components tested were teichoic acids α, β, and combinations of both and protein A. When used in equivalent amounts, these were found to have no effect on the removal of serum factors necessary for phagocytosis and intracellular killing of S. aureus.

Full text

PDF
752

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BADDILEY J., BUCHANAN J. G., RAJBHANDARY U. L., SANDERSON A. R. Teichoic acid from the walls of Staphylococcus aureus H. Structure of the N-acetylglucosaminyl-ribitol residues. Biochem J. 1962 Mar;82:439–448. doi: 10.1042/bj0820439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLEIWEIS A. S., KARAKAWA W. W., KRAUSE R. M. IMPROVED TECHNIQUE FOR THE PREPARATION OF STREPTOCOCCAL CELL WALLS. J Bacteriol. 1964 Oct;88:1198–1200. doi: 10.1128/jb.88.4.1198-1200.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chipman D. M., Sharon N. Mechanism of lysozyme action. Science. 1969 Aug 1;165(3892):454–465. doi: 10.1126/science.165.3892.454. [DOI] [PubMed] [Google Scholar]
  4. Dossett J. H., Kronvall G., Williams R. C., Jr, Quie P. G. Antiphagocytic effects of staphylococcal protein A. J Immunol. 1969 Dec;103(6):1405–1410. [PubMed] [Google Scholar]
  5. Ensign J. C., Wolfe R. S. Characterization of a small proteolytic enzyme which lyses bacterial cell walls. J Bacteriol. 1966 Feb;91(2):524–534. doi: 10.1128/jb.91.2.524-534.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Forsgren A. Protein A from Staphylococcus aureus. VI. Reaction with subunits from guinea pig gamma-1- and gamma-2-globulin. J Immunol. 1968 May;100(5):927–930. [PubMed] [Google Scholar]
  7. Forsgren A., Sjöquist J. "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin. J Immunol. 1966 Dec;97(6):822–827. [PubMed] [Google Scholar]
  8. Forsgren A., Sjöquist J. "Protein A" from Staphylococcus aureus. 3. Reaction with rabbit gamma-globulin. J Immunol. 1967 Jul;99(1):19–24. [PubMed] [Google Scholar]
  9. Lind I., Mansa B. Further investigation of specific and non-specific adsorption of serum globulins to Staphylococcus aureus. Acta Pathol Microbiol Scand. 1968;73(4):637–645. doi: 10.1111/j.1699-0463.1968.tb03221.x. [DOI] [PubMed] [Google Scholar]
  10. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MUDD S., YOSHIDA A., LI I. W., LENHART N. A. IDENTIFICATION OF A SOMATIC ANTIGEN OF STAPHYLOCOCCUS AUREUS CRITICAL FOR PHAGOCYTOSIS BY HUMAN BLOOD LEUCOCYTES. Nature. 1963 Sep 21;199:1200–1201. doi: 10.1038/1991200a0. [DOI] [PubMed] [Google Scholar]
  12. Martin R. R., White A. Prevention of staphylococcal bacteriophage activity by antigen A precipitins in human sera. J Bacteriol. 1968 Jun;95(6):2177–2181. doi: 10.1128/jb.95.6.2177-2181.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NATHENSON S. G., STROMINGER J. L. Enzymatic synthesis and immunochemistry of N-acetylglucosaminylribitol linkages in the teichoic acids of Staphylococcus aureus stains. J Biol Chem. 1962 Dec;237:3839–3841. [PubMed] [Google Scholar]
  14. POMALES-LEBRON A., STINEBRING W. R. Intracellular multiplication of Brucella abortus in normal and immune mononuclear phagocytes. Proc Soc Exp Biol Med. 1957 Jan;94(1):78–83. doi: 10.3181/00379727-94-22860. [DOI] [PubMed] [Google Scholar]
  15. Shayegani M. Failure of Immune Sera to Enhance Significantly Phagocytosis of Staphylococus aureus: Nonspecific Adsorption of Phagocytosis-Promoting Factors. Infect Immun. 1970 Dec;2(6):742–749. doi: 10.1128/iai.2.6.742-749.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tipper D. J., Strominger J. L., Ensign J. C. Structure of the cell wall of Staphylococcus aureus, strain Copenhagen. VII. Mode of action of the bacteriolytic peptidase from Myxobacter and the isolation of intact cell wall polysaccharides. Biochemistry. 1967 Mar;6(3):906–920. doi: 10.1021/bi00855a035. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES