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. 1971 Jun;3(6):723–726. doi: 10.1128/iai.3.6.723-726.1971

Fatty Acid Requirements of the Kazan 5 and Reiter Strains of Treponema pallidum

Russell C Johnson 1, Laura M Eggebraten 1
PMCID: PMC416228  PMID: 16558045

Abstract

The fatty acid requirements of two avirulent treponemes were investigated by using a “lipid-poor” albumin-thioglycolate medium. The Kazan 5 and the Reiter stains of Treponema pallidum required a pair of fatty acids for growth. One member of the pair was saturated and the other was unsaturated. The saturated fatty acids could contain either an odd or even number of carbon atoms, but a chain length of at least 14 carbon atoms was necessary. Unsaturated fatty acids with one, two, or three double bonds were satisfactory if they contained 15 or more carbon atoms. The pair of fatty acids could be replaced with a single 18-carbon monounsaturated fatty acid if it was in the trans configuration rather than the naturally occurring cis form.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Canale-Parola E., Udris Z., Mandel M. The classification of free-living spirochetes. Arch Mikrobiol. 1968;63(4):385–397. doi: 10.1007/BF00412124. [DOI] [PubMed] [Google Scholar]
  2. Johnson R. C., Harris V. G., Walby J. K. Characterization of leptospires according to fatty acid requirements. J Gen Microbiol. 1969 Mar;55(3):399–407. doi: 10.1099/00221287-55-3-399. [DOI] [PubMed] [Google Scholar]
  3. Johnson R. C., Livermore B. P., Jenkin H. M., Eggebraten L. Lipids of Treponema pallidum Kazan 5. Infect Immun. 1970 Nov;2(5):606–609. doi: 10.1128/iai.2.5.606-609.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Johnson R. C., Livermore B. P., Walby J. K., Jenkin H. M. Lipids of parasitic and saprophytic leptospires. Infect Immun. 1970 Sep;2(3):286–291. doi: 10.1128/iai.2.3.286-291.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Meyer H., Meyer F. Biosynthesis of unsaturated fatty acids by a free-living spirochete. Biochim Biophys Acta. 1969 Jan 21;176(1):202–204. doi: 10.1016/0005-2760(69)90091-5. [DOI] [PubMed] [Google Scholar]
  6. OYAMA V. I., STEINMAN H. G., EAGLE H. The nutritional requirements of treponemata. V. A detoxified lipide as the essential growth factor supplied by crystallized serum albumin. J Bacteriol. 1953 May;65(5):609–616. doi: 10.1128/jb.65.5.609-616.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. POWER D. A., PELCZAR M. J., Jr Growth enhancement of the Reiter treponeme by fatty acids. J Bacteriol. 1959 Jun;77(6):789–793. doi: 10.1128/jb.77.6.789-793.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Razin S. Structure and function in mycoplasma. Annu Rev Microbiol. 1969;23:317–356. doi: 10.1146/annurev.mi.23.100169.001533. [DOI] [PubMed] [Google Scholar]
  9. Rodwall A. Fatty-acid composition of Mycoplasma lipids: biomembrane with only 1 fatty acid. Science. 1968 Jun 21;160(3834):1350–1351. doi: 10.1126/science.160.3834.1350. [DOI] [PubMed] [Google Scholar]
  10. Roessler W. G., Brewer C. R. Permanent turbidity standards. Appl Microbiol. 1967 Sep;15(5):1114–1121. doi: 10.1128/am.15.5.1114-1121.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stern N., Shenberg E., Tietz A. Studies on the metabolism of fatty acids in Leptospira: the biosynthesis of delta 9- and delta 11-monounsaturated acids. Eur J Biochem. 1969 Mar;8(1):101–108. doi: 10.1111/j.1432-1033.1969.tb00501.x. [DOI] [PubMed] [Google Scholar]

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