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. 1971 Aug;4(2):110–115. doi: 10.1128/iai.4.2.110-115.1971

Production of Extracellular Enzymes and Enterotoxins A, B, and C by Staphylococcus aureus

Audrey W Jarvis 1, R C Lawrence 1
PMCID: PMC416273  PMID: 5005289

Abstract

Eighty-seven strains of Staphylococcus aureus were examined for their ability to produce enterotoxins A, B, and C, deoxyribonuclease, lysozyme, proteinase, lipase, and α-, β-, and δ-hemolysins. Enterotoxigenic strains showed a significant tendency to be high lipase producers, but none of the other enzymes formed were correlated with the ability of the staphylococci to produce enterotoxins A, B, or C. The conversion of ent to ent+ strains by lysogenization did not affect significantly the ability of the strains to produce any of the above extracellular enzymes. The formation of enzymes such as deoxyribonuclease and lysozyme by staphylococci is not therefore an indication, necessarily, of their potential enterotoxigenicity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLAIR J. E., CARR M. Lysogeny in staphylococci. J Bacteriol. 1961 Dec;82:984–993. doi: 10.1128/jb.82.6.984-993.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Casman E. P. Staphylococcal enterotoxin. Ann N Y Acad Sci. 1965 Jul 23;128(1):124–131. doi: 10.1111/j.1749-6632.1965.tb11634.x. [DOI] [PubMed] [Google Scholar]
  3. Chesbro W. R., Auborn K. Enzymatic detection of the growth of Staphylococcus aureus in foods. Appl Microbiol. 1967 Sep;15(5):1150–1159. doi: 10.1128/am.15.5.1150-1159.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ELEK S. D., LEVY E. The nature of discrepancies between haemolysins in culture filtrates and plate haemolysin patterns of staphylococci. J Pathol Bacteriol. 1954 Jul;68(1):31–40. doi: 10.1002/path.1700680105. [DOI] [PubMed] [Google Scholar]
  5. Hawiger J. Frequency of staphylococcal lysozyme production tested by plate method. J Clin Pathol. 1968 May;21(3):390–393. doi: 10.1136/jcp.21.3.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jarvis A. W., Lawrence R. C. A micro-method for the quantitative determination of deoxyribonuclease. Can J Biochem. 1969 Jun;47(6):673–675. doi: 10.1139/o69-103. [DOI] [PubMed] [Google Scholar]
  7. Jarvis A. W., Lawrence R. C. Production of high titers of enterotoxins for the routine testing of staphylococci. Appl Microbiol. 1970 Apr;19(4):698–699. doi: 10.1128/am.19.4.698-699.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lawrence R. C., Fryer T. F., Reiter B. The production and characterization of lipases from a micrococcus and a pseudomonad. J Gen Microbiol. 1967 Sep;48(3):401–418. doi: 10.1099/00221287-48-3-401. [DOI] [PubMed] [Google Scholar]
  9. Liu P. V., Hsieh H. C. Inhibition of protease production of various bacteria by ammonium salts: its effect on toxin production and virulence. J Bacteriol. 1969 Aug;99(2):406–413. doi: 10.1128/jb.99.2.406-413.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martley F. G., Jarvis A. W., Bacon D. F., Lawrence R. C. Typing of coagulase-positive staphylococci by proteolytic activity on buffered caseinate-agar, with special reference to bacteriophage nontypable strains. Infect Immun. 1970 Oct;2(4):439–442. doi: 10.1128/iai.2.4.439-442.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martley F. G., Jayashankar S. R., Lawrence R. C. An improved agar medium for the detection of proteolytic organisms in total bacterial counts. J Appl Bacteriol. 1970 Jun;33(2):363–370. doi: 10.1111/j.1365-2672.1970.tb02208.x. [DOI] [PubMed] [Google Scholar]
  12. RICHMOND M. H. Formation of a lytic enzyme by a strain of Bacillus subtilis. Biochim Biophys Acta. 1959 May;33(1):78–92. doi: 10.1016/0006-3002(59)90500-1. [DOI] [PubMed] [Google Scholar]
  13. STADTMAN E. R., NOVELLI G. D., LIPMANN F. Coenzyme A function in and acetyl transfer by the phosphotransacetylase system. J Biol Chem. 1951 Jul;191(1):365–376. [PubMed] [Google Scholar]
  14. SUGIYAMA H., BERGDOLL M. S., DACK G. M. In vitro studies on staphylococcal enterotoxin production. J Bacteriol. 1960 Aug;80:265–270. doi: 10.1128/jb.80.2.265-270.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Victor R., Lachica F., Weiss K. F., Deibel R. H. Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus. Appl Microbiol. 1969 Jul;18(1):126–127. doi: 10.1128/am.18.1.126-127.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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