Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Mar;13(3):647–652. doi: 10.1128/iai.13.3.647-652.1976

Formation of cross-reacting antibodies against cellular and extracellular lipoteichoic acid of Streptococcus mutans BHT.

K W Knox, J L Markham, A J Wicken
PMCID: PMC420659  PMID: 1270127

Abstract

Antisera prepared against strains of Streptococcus mutans, particularly strain BHT, contain antibodies to the membrane lipoteichoic acid component that will cross-react with lipoteichoic acids from three species of lactobacilli. A more detailed study of the antibodies to strain BHT confirmed that the antibodies are specific for the polyglycerol phosphate component common to the lipoteichoic acids. Sera with a higher hemagglutinating titer could be obtained by injecting disrupted organisms. The partially purified lipoteichoic acid isolated from the culture fluid of S. mutans BHT was also immunogenic antibodies; antibodies were also formed against another unidentified component in this extracellular fraction.

Full text

PDF
650

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berglund S. E. Introduction to Conference on The Implications of Immune Reactions in the Pathogenesis of Periodontal Disease. J Periodontol. 1970 Apr;41(4):195–196. doi: 10.1902/jop.1970.41.4.195. [DOI] [PubMed] [Google Scholar]
  2. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  3. Bratthall D. Immunofluorescent identification of Streptococcus mutans. Odontol Revy. 1972;23(2):181–196. [PubMed] [Google Scholar]
  4. Hardie J. M., Bowden G. H. Cell wall and serological studies on Streptococcus mutans. Caries Res. 1974;8(4):301–316. doi: 10.1159/000260120. [DOI] [PubMed] [Google Scholar]
  5. Hewett M. J., Knox K. W., Wicken A. J. Studies on the group F antigen of lactobacilli: detection of antibodies by haemagglutination. J Gen Microbiol. 1970 Mar;60(3):315–322. doi: 10.1099/00221287-60-3-315. [DOI] [PubMed] [Google Scholar]
  6. Joseph R., Shockman G. D. Synthesis and excretion of glycerol teichoic acid during growth of two streptococcal species. Infect Immun. 1975 Aug;12(2):333–338. doi: 10.1128/iai.12.2.333-338.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Knox K. W., Hewett M. J., Wicken A. J. Studies on the group F antigen of lactobacilli: antigenicity and serological specificity of teichoic acid preparations. J Gen Microbiol. 1970 Mar;60(3):303–313. doi: 10.1099/00221287-60-3-303. [DOI] [PubMed] [Google Scholar]
  8. Knox K. W., Wicken A. J. Immunological properties of teichoic acids. Bacteriol Rev. 1973 Jun;37(2):215–257. doi: 10.1128/br.37.2.215-257.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Knox K. W., Wicken A. J. Serological studies on the teichoic acids of Lactobacillus plantarum. Infect Immun. 1972 Jul;6(1):43–49. doi: 10.1128/iai.6.1.43-49.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Linzer R., Slade H. D. Purification and characterization of Streptococcus mutans group d cell wall polysaccharide antigen. Infect Immun. 1974 Aug;10(2):361–368. doi: 10.1128/iai.10.2.361-368.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Markham J. L., Knox K. W., Schamschula R. G., Wicken A. J. Antibodies to teichoic acids in man. Arch Oral Biol. 1973 Mar;18(3):313–319. doi: 10.1016/0003-9969(73)90153-2. [DOI] [PubMed] [Google Scholar]
  12. Markham J. L., Knox K. W., Wicken A. J., Hewett M. J. Formation of extracellular lipoteichoic acid by oral streptococci and lactobacilli. Infect Immun. 1975 Aug;12(2):378–386. doi: 10.1128/iai.12.2.378-386.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mukasa H., Slade H. D. Extraction, purification, and chemical and immunological properties of the Streptococcus mutans group "a" polysaccharide cell wall antigen. Infect Immun. 1973 Aug;8(2):190–198. doi: 10.1128/iai.8.2.190-198.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mukasa H., Slade H. D. Structure and immunological specificity of the Streptococcus mutans group b cell wall antigen. Infect Immun. 1973 Apr;7(4):578–585. doi: 10.1128/iai.7.4.578-585.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosan B. Antigens of Streptococcus sanguis. Infect Immun. 1973 Feb;7(2):205–211. doi: 10.1128/iai.7.2.205-211.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SLADE H. D., SLAMP W. C. Cell-wall composition and the grouping antigens of Streptococci. J Bacteriol. 1962 Aug;84:345–351. doi: 10.1128/jb.84.2.345-351.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sharpe M. E., Brock J. H., Knox K. W., Wicken A. J. Glycerol teichoic acid as a common antigenic factor in lactobacilli and some other gram-positive organisms. J Gen Microbiol. 1973 Jan;74(1):119–126. doi: 10.1099/00221287-74-1-119. [DOI] [PubMed] [Google Scholar]
  18. Van de Rijn I., Bleiweis A. S. Antigens of Streptococcus mutans. I. Characterization of a serotype-specific determinant from Streptococcus mutans. Infect Immun. 1973 May;7(5):795–804. doi: 10.1128/iai.7.5.795-804.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vaught R. M., Bleiweis A. S. Antigens of Streptococcus mutans. II. Characterization of an antigen resembling a glycerol teichoic acid in walls of strain BHT. Infect Immun. 1974 Jan;9(1):60–67. doi: 10.1128/iai.9.1.60-67.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wicken A. J., Gibbens J. W., Knox K. W. Comparative studies on the isolation of membrane lipoteichoic acid from Lactobacillus fermenti. J Bacteriol. 1973 Jan;113(1):365–372. doi: 10.1128/jb.113.1.365-372.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wicken A. J., Knox K. W. A serological comparison of the membrane teichoic acids from lactobacilli of different serological groups. J Gen Microbiol. 1971 Aug;67(2):251–254. doi: 10.1099/00221287-67-2-251. [DOI] [PubMed] [Google Scholar]
  22. Wicken A. J., Knox K. W. Characterization of group N streptococcus lipoteichoic acid. Infect Immun. 1975 May;11(5):973–981. doi: 10.1128/iai.11.5.973-981.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wicken A. J., Knox K. W. Lipoteichoic acids: a new class of bacterial antigen. Science. 1975 Mar 28;187(4182):1161–1167. doi: 10.1126/science.46620. [DOI] [PubMed] [Google Scholar]
  24. van Houte J., Saxton C. A. Cell wall thickening and intracellular polysaccharide in microorganisms of the dental plaque. Caries Res. 1971;5(1):30–43. doi: 10.1159/000259730. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES