Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Apr;13(4):1103–1109. doi: 10.1128/iai.13.4.1103-1109.1976

Interaction of Chlamydia trachomatis organisms and HeLa 229 cells.

C C Kuo, T Grayston
PMCID: PMC420724  PMID: 179950

Abstract

The infection of HeLa 229 cells in monolayer culture with trachoma (B/TW-5/OT) and lymphogranuloma venereum (LGV) (L2/434/Bu) organism was studied in terms of two parameters: radioactivity counts of cell-associated tritium labeled organisms at the initial stage of inoculation for measurement of attachment, and inclusion counts of infection cells after incubation for measurement of growth. Factors affecting attachment and inclusion formation and correlation of the two are presented. It was shown that attachment is an important initial step in infection by Chlamydia trachomatis. The rate of attachment was temperature dependent. The attachment of LGV organisms was affected more profoundly by temperature than was that of trachoma organisms. Attachment and inclusion formation of trachoma and LGV organisms were inhibited by heparin. Diethylaminoethyl-dextran was again shown to enhance attachment and inclusion formation of trachoma but not LGV organisms. NaF had no effect on attachment, but inhibited inclusion formation of both trachoma and LGV organisms. Both attachment and inclusion formation of trachoma organisms were strongly enhanced by centrifugation of the inoculum onto the cell monolayer. Although inclusion formation of trachoma organism was much greater in susceptible cells (HeLa 229) than relatively insusceptible cells (fetal tonsil), attachment was only slightly greater. The results based on the test of two cell lines suggested that attachment prpbably is not a critical factor in determing a cell line's susceptibility to infection with trachoma organisms.

Full text

PDF
1109

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander E. R., Wang S. P., Grayston J. T. Further classification of TRIC agents from ocular trachoma and other sources by the mouse toxicity prevention test. Am J Ophthalmol. 1967 May;63(5 Suppl):1469–1478. doi: 10.1016/0002-9394(67)94133-5. [DOI] [PubMed] [Google Scholar]
  2. Alexander J. J. Separation of protein synthesis in meningopneumonitisgent from that in L cells by differential susceptibility to cycloheximide. J Bacteriol. 1968 Feb;95(2):327–332. doi: 10.1128/jb.95.2.327-332.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Becker Y., Asher Y. Synthesis of trachoma agent proteins in emetine-treated cells. J Bacteriol. 1972 Mar;109(3):966–970. doi: 10.1128/jb.109.3.966-970.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Becker Y., Hochberg E., Zakay-Rones Z. Interaction of trachoma elementary bodies with host cells. Isr J Med Sci. 1969 Jan-Feb;5(1):121–124. [PubMed] [Google Scholar]
  5. Croy T. R., Kuo C. C., Wang S. P. Comparative susceptibility of eleven mammalian cell lines to infection with trachoma organisms. J Clin Microbiol. 1975 May;1(5):434–439. doi: 10.1128/jcm.1.5.434-439.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friis R. R. Interaction of L cells and Chlamydia psittaci: entry of the parasite and host responses to its development. J Bacteriol. 1972 May;110(2):706–721. doi: 10.1128/jb.110.2.706-721.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GORDON F. B., QUAN A. L. ISOLATION OF THE TRACHOMA AGENT IN CELL CULTURE. Proc Soc Exp Biol Med. 1965 Feb;118:354–359. doi: 10.3181/00379727-118-29841. [DOI] [PubMed] [Google Scholar]
  8. HIGASHI N., TAMURA A., IWANAGA M. Developmental cycle and reproductive mechanism of the meningopneumonitis virus in strain L cells. Ann N Y Acad Sci. 1962 Mar 5;98:100–121. doi: 10.1111/j.1749-6632.1962.tb30536.x. [DOI] [PubMed] [Google Scholar]
  9. Harrison M. J. Enhancing effect of DEAE-Dextran on inclusion counts of an ovine Chlamydia (Bedsonia) in cell culture. Aust J Exp Biol Med Sci. 1970 Apr;48(2):207–213. doi: 10.1038/icb.1970.20. [DOI] [PubMed] [Google Scholar]
  10. Kuo C. C., Wang S. P., Grayston J. T. Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction. Infect Immun. 1973 Jul;8(1):74–79. doi: 10.1128/iai.8.1.74-79.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuo C., Wang S., Grayston J. T. Differentiation of TRIC and LGV organisms based on enhancement of infectivity by DEAE-dextran in cell culture. J Infect Dis. 1972 Mar;125(3):313–317. doi: 10.1093/infdis/125.3.313. [DOI] [PubMed] [Google Scholar]
  12. Kuo C., Wang S., Wentworth B. B., Grayston J. T. Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. J Infect Dis. 1972 Jun;125(6):665–668. doi: 10.1093/infdis/125.6.665. [DOI] [PubMed] [Google Scholar]
  13. Lonberg-Holm K., Philipson L. Early interaction between animal viruses and cells. Monogr Virol. 1974;9:1–148. [PubMed] [Google Scholar]
  14. Rota T. R., Nichols R. L. Infection of cell cultures by trachoma agent: enhancement by DEAE-dextran. J Infect Dis. 1971 Oct;124(4):419–421. doi: 10.1093/infdis/124.4.419. [DOI] [PubMed] [Google Scholar]
  15. SBARRA A. J., KARNOVSKY M. L. The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem. 1959 Jun;234(6):1355–1362. [PubMed] [Google Scholar]
  16. Schachter J., Meyer K. F. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J Bacteriol. 1969 Sep;99(3):636–638. doi: 10.1128/jb.99.3.636-638.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WEISS E., DRESSLER H. R. Centrifugation and Rickettsiae and viruses onto cells and its effect on infection. Proc Soc Exp Biol Med. 1960 Apr;103:691–695. doi: 10.3181/00379727-103-25637. [DOI] [PubMed] [Google Scholar]
  18. Wang S. P., Grayston J. T. A potency test for trachoma vaccine utilizing the mouse toxicity prevention test. Am J Ophthalmol. 1967 May;63(5 Suppl):1443–1454. doi: 10.1016/0002-9394(67)94130-x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES