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. 1976 Sep;14(3):603–606. doi: 10.1128/iai.14.3.603-606.1976

Assay of bovine interferons in cultures of the porcine cell line IB-RS-2.

R Ahl, A Rump
PMCID: PMC420927  PMID: 184049

Abstract

An assay for bovine interferons has been developed using the porcine cell line IB-RS-2 and a bovine enterovirus, CBV-D, as challenge virus. The method is based on estimation of cytopathic effect measured by uptake of neutral red. Teh assay is simple, sensitive, and reproducible. A comparative test of different viruses in IB-RS-2 cells and secondary calf kidney cells revealed that the sensitivity of a virus to interferon can vary up to 1,000-fold in the two cell systems. Vesicular stomatitis virus was found to be rather insensitive to interferon in IB-RS-2 cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahl R. Inhibitors of foot-and-mouth disease virus. I. Effect of EDTA, temperature, and interferon on the viral growth cycle. Arch Gesamte Virusforsch. 1974;46(3-4):302–314. doi: 10.1007/BF01240072. [DOI] [PubMed] [Google Scholar]
  2. Ahl R., Straub O. C. Die lokale Interferonbildung im Respirations- und Genitaltrakt nach experimenteller Infektion mit Rhinotracheitis (IBR)- und Bläschenausschlag (IPV)-Virus. Dtsch Tierarztl Wochenschr. 1971 Dec 15;78(24):653–655. [PubMed] [Google Scholar]
  3. Armstrong J. A., Porterfield J. S., De Madrid A. T. C-type virus particles in pig kidney cell lines. J Gen Virol. 1971 Feb;10(2):195–198. doi: 10.1099/0022-1317-10-2-195. [DOI] [PubMed] [Google Scholar]
  4. Armstrong J. A. Semi-micro, dye-binding assay for rabbit interferon. Appl Microbiol. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DINTER Z., PHILIPSON L. An interferon produced by foot and mouth disease virus (FMDV) in calf kidney cells. Proc Soc Exp Biol Med. 1962 Apr;109:893–897. doi: 10.3181/00379727-109-27370. [DOI] [PubMed] [Google Scholar]
  6. Dahl H., Degré M. A micro assay for mouse and human interferon. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(6):863–870. doi: 10.1111/j.0365-5563.1973.tb00012.x. [DOI] [PubMed] [Google Scholar]
  7. De Castro M. P. An infectious agent causing "spontaneous" degeneration of swine cells in vitro. In Vitro. 1973 Jul-Aug;9(1):8–16. doi: 10.1007/BF02615982. [DOI] [PubMed] [Google Scholar]
  8. Gresser I., Bandu M. T., Brouty-boye D., Tovey M. Pronounced antiviral activity of human interferon on bovine and porcine cells. Nature. 1974 Oct 11;251(5475):543–545. doi: 10.1038/251543a0. [DOI] [PubMed] [Google Scholar]
  9. HERMODSSON S. ACTION OF PARAINFLUENZA VIRUS TYPE 3 ON SYNTHESIS OF INTERFERON AND MULTIPLICATION OF HETEROLOGOUS VIRUSES. Acta Pathol Microbiol Scand. 1964;62:224–238. doi: 10.1111/apm.1964.62.2.224. [DOI] [PubMed] [Google Scholar]
  10. HERMODSSON S., PHILIPSON L. A SENSITIVE METHOD FOR INTERFERON ASSAY. Proc Soc Exp Biol Med. 1963 Dec;114:574–579. doi: 10.3181/00379727-114-28735. [DOI] [PubMed] [Google Scholar]
  11. Hallum J. V., Thacore H. R., Youngner J. S. Factors affecting the sensitivity of different viruses to interferon. J Virol. 1970 Aug;6(2):156–162. doi: 10.1128/jvi.6.2.156-162.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ley K. D., Burger D., Henson J. B. Equine interferon: characterization of a viral inhibitor induced in equine kidney cell cultures with statolon. J Infect Dis. 1970 Mar;121(3):335–338. doi: 10.1093/infdis/121.3.335. [DOI] [PubMed] [Google Scholar]
  13. Nardelli L., Lodetti E., Gualandi G. L., Burrows R., Goodridge D., Brown F., Cartwright B. A foot and mouth disease syndrome in pigs caused by an enterovirus. Nature. 1968 Sep 21;219(5160):1275–1276. doi: 10.1038/2191275a0. [DOI] [PubMed] [Google Scholar]
  14. POLLIKOFF R., DONIKIAN M. A., PADRON A., LIU O. C. Tissue specificity of interferon prepared in various tissue cultures. Proc Soc Exp Biol Med. 1962 Jun;110:232–234. doi: 10.3181/00379727-110-27476. [DOI] [PubMed] [Google Scholar]
  15. Pidot A. L. Dye uptake assay: an efficient and sensitive method for human interferon titration. Appl Microbiol. 1971 Oct;22(4):671–677. doi: 10.1128/am.22.4.671-677.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Richmond J. Y. An interferon-like inhibitor of foot-and-mouth disease virus induced by phytohemagglutinin in swine leukocyte cultures. Arch Gesamte Virusforsch. 1969;27(2):282–289. doi: 10.1007/BF01249650. [DOI] [PubMed] [Google Scholar]
  17. STOKER M., MACPHERSON I. SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES. Nature. 1964 Sep 26;203:1355–1357. doi: 10.1038/2031355a0. [DOI] [PubMed] [Google Scholar]
  18. Sellers R. F., Herniman K. A., Hawkins C. W. The effect of a synthetic anionic polymer (pyran) on the development of foot-and-mouth disease in guinea-pigs, cattle and pigs. Res Vet Sci. 1972 Jul;13(4):339–341. [PubMed] [Google Scholar]
  19. Stewart W. E., 2nd, Scott W. D., Sulkin S. E. Relative sensitivities of viruses to different species of interferon. J Virol. 1969 Aug;4(2):147–153. doi: 10.1128/jvi.4.2.147-153.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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