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. 1977 May;16(2):655–661. doi: 10.1128/iai.16.2.655-661.1977

Thymidine kinaseless revertants of Ltk- cells transformed by herpes simplex virus type 1 are resistant to retransformation by homologous virus.

K C Chadha, W H Munyon, R G Hughes Jr
PMCID: PMC421006  PMID: 193795

Abstract

Mouse L cells lacking thymidine kinase (Ltk-) that had been transformed to the thymidine kinase-positive (tk+) phenotype by herpes simplex virus type 1 (HSV-1) were cultured in medium containing tritiated thymidine. Six clonal lines of cells surviving this treatment were found to have the following properties: (i) the cells were tk- and had spontaneous back-reversion frequencies to the tk+ phenotype of 10(-5) or less, (ii) the cells contained HSV antigens, although in lesser amounts than in the parental transformed cells, and (iii) the cells were retransformable to the tk+ phenotype by HSV-1 at frequencies of about 1 to 13% of the frequency of the primary transformation of LtK- cells. HSV-1 plaqued as efficiently on monolayers of these cells and replicated in them to the same extent as it did in Ltk- cells. These results indicate that HSV-1-transformed L cells surviving selection with tritiated thymidine are unlike the parental Ltk- cells in that they are damaged in such a way that the cells are resistant to retransformation by homologous virus, although they remain fully permissive for virus replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berk A. J., Clayton D. A. A genetically distinct thymidine kinase in mammalian mitochondria. Exclusive labeling of mitochondrial deoxyribonucleic acid. J Biol Chem. 1973 Apr 25;248(8):2722–2729. [PubMed] [Google Scholar]
  2. Breslow R. E., Goldsby R. A. Isolation and characterization of thymidine transport mutants of Chinese hamster cells. Exp Cell Res. 1969 Jun;55(3):339–346. doi: 10.1016/0014-4827(69)90567-9. [DOI] [PubMed] [Google Scholar]
  3. Chadha K. C., Munyon W. H., Zeigel R. F. Expression of type-specific virion structural antigen(s) in L cells transformed by herpes simplex virus types 1 and 2. Virology. 1977 Jan;76(1):433–436. doi: 10.1016/0042-6822(77)90317-8. [DOI] [PubMed] [Google Scholar]
  4. Chadha K. C., Munyon W. Presence of herpes simplex virus-related antigens in transformed L cells. J Virol. 1975 Jun;15(6):1475–1486. doi: 10.1128/jvi.15.6.1475-1486.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davidson R. L., Adelstein S. J., Oxman M. N. Herpes simplex virus as a source of thymidine kinase for thymidine kinase-deficient mouse cells: suppression and reactivation of the viral enzyme. Proc Natl Acad Sci U S A. 1973 Jul;70(7):1912–1916. doi: 10.1073/pnas.70.7.1912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis D. B., Kingsbury D. T. Quantitation of the viral DNA present in cells transformed by UV-irradiated herpes simplex virus. J Virol. 1976 Mar;17(3):788–793. doi: 10.1128/jvi.17.3.788-793.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davis D. B., Munyon W., Buchsbaum R., Chawda R. Virus type-specific thymidine kinase in cells biochemically transformed by herpes simplex virus types 1 and 2. J Virol. 1974 Jan;13(1):140–145. doi: 10.1128/jvi.13.1.140-145.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duff R., Rapp F. Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2. J Virol. 1971 Oct;8(4):469–477. doi: 10.1128/jvi.8.4.469-477.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  10. Frenkel N., Locker H., Cox B., Roizman B., Rapp F. Herpes simplex virus DNA in transformed cells: sequence complexity in five hamster cell lines and one derived hamster tumor. J Virol. 1976 Jun;18(3):885–893. doi: 10.1128/jvi.18.3.885-893.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hughes R. G., Munyon W. H. Mutant of herpes simplex virus type 1 conditionally able to transform thymidine kinaseless L cells to a tk+ phenotype. J Virol. 1976 Jun;18(3):867–872. doi: 10.1128/jvi.18.3.867-872.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kraiselburd E., Gage L. P., Weissbach A. Presence of a herpes simplex virus DNA fragment in an L cell clone obtained after infection with irradiated herpes simplex virus I. J Mol Biol. 1975 Oct 5;97(4):533–542. doi: 10.1016/s0022-2836(75)80057-x. [DOI] [PubMed] [Google Scholar]
  13. Lin S. S., Munyon W. Expression of the viral thymidine kinase gene in herpes simplex virus-transformed L cells. J Virol. 1974 Nov;14(5):1199–1208. doi: 10.1128/jvi.14.5.1199-1208.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Munyon W., Buchsbaum R., Paoletti E., Mann J., Kraiselburd E., Davis D. Electrophoresis of thymidine kinase activity synthesized by cells transformed by herpes simplex virus. Virology. 1972 Sep;49(3):683–689. doi: 10.1016/0042-6822(72)90525-9. [DOI] [PubMed] [Google Scholar]
  15. Munyon W., Kraiselburd E., Davis D., Mann J. Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus. J Virol. 1971 Jun;7(6):813–820. doi: 10.1128/jvi.7.6.813-820.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Prasad I., Zouzias D., Basilico C. Simian virus 40 integration sites in the genome of virus-transformed mouse cells. J Virol. 1975 Oct;16(4):897–904. doi: 10.1128/jvi.16.4.897-904.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thouless M. E., Chadha K. C., Munyon W. H. Serological specificity of thymidine kinase activity in herpes simplex virus-transformed L cells. Virology. 1976 Jan;69(1):350–351. doi: 10.1016/0042-6822(76)90225-7. [DOI] [PubMed] [Google Scholar]

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