Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Jul;17(1):16–20. doi: 10.1128/iai.17.1.16-20.1977

Immunization with R mutants of Salmonella minnesota. II. Comparison of the protective effect of immunization with lipid A and the Re mutant.

S C Bruins, R Stumacher, M A Johns, W R McCabe
PMCID: PMC421075  PMID: 407161

Abstract

The protective effect of active immunization with Salmonella minnesota Re bacilli or lipid A was assessed in the granulocytopenic rabbit model. Animals were immunized with Re bacilli, lipid A, or Pseudomonas aeruginosa as a nonspecific immunogen. After colonization with one of three enterobacterial strains (two Escherichia coli, one Enterobacter aerogenes), the immunized rabbits as well as controls given saline injections were made leukopenic with nitrogen mustard and monitored for fever, bacteremia, and death. Survival rates were significantly greater in Re-immunized animals than in saline controls or P. aeruginosa-immunized animals. Immunization with lipid A afforded no protection. In addition, rabbits immunized with Re mutant bacilli developed bacteremia less frequently than the others, indicating that antibody to Re may inhibit invasion of the intestinal mucosa as well as protect after bacteremia has developed.

Full text

PDF
19

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braude A. I., Douglas H., Jones J. Experimental production of lethal Escherichia coli bacteremia of pelvic origin. J Bacteriol. 1969 Jun;98(3):979–991. doi: 10.1128/jb.98.3.979-991.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braude A. I., Douglas H. Passive immunization against the local Shwartzman reaction. J Immunol. 1972 Feb;108(2):505–512. [PubMed] [Google Scholar]
  3. Chedid L., Parant M., Parant F., Boyer F. A proposed mechanism for natural immunity to enterobacterial pathogens. J Immunol. 1968 Feb;100(2):292–306. [PubMed] [Google Scholar]
  4. Galanos C., Lüderitz O., Westphal O. Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides. Eur J Biochem. 1971 Dec 22;24(1):116–122. doi: 10.1111/j.1432-1033.1971.tb19661.x. [DOI] [PubMed] [Google Scholar]
  5. Johns M. A., Bruins S. C., McCabe W. R. Immunization with R mutants of Salmonella minnesota. II. Serological response to lipid A and the lipopolysaccharide of Re mutants. Infect Immun. 1977 Jul;17(1):9–15. doi: 10.1128/iai.17.1.9-15.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McCabe W. R. Immunization with R mutants of S. Minnesota. I. Protection against challenge with heterologous gram-negative bacilli. J Immunol. 1972 Mar;108(3):601–610. [PubMed] [Google Scholar]
  8. McCabe W. R., Kreger B. E., Johns M. Type-specific and cross-reactive antibodies in gram-negative bacteremia. N Engl J Med. 1972 Aug 10;287(6):261–267. doi: 10.1056/NEJM197208102870601. [DOI] [PubMed] [Google Scholar]
  9. Mullan N. A., Newsome P. M., Cunnington P. G., Palmer G. H., Wilson M. E. Protection against gram-negative infections with antiserum to lipid A from Salmonella minnesota R595. Infect Immun. 1974 Dec;10(6):1195–1201. doi: 10.1128/iai.10.6.1195-1201.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NETER E., BERTRAM L. F., ZAK D. A., MURDOCK M. R., ARBESMAN C. E. Studies on hemagglutination and hemolysis by escherichia coli antisera. J Exp Med. 1952 Jul;96(1):1–15. doi: 10.1084/jem.96.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rietschel E. T., Kim Y. B., Watson D. W., Galanos C., Lüderitz O., Westphal O. Pyrogenicity and immunogenicity of lipid A complexed with bovine serum albumin or human serum albumin. Infect Immun. 1973 Aug;8(2):173–177. doi: 10.1128/iai.8.2.173-177.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tate W. J., 3rd, Douglas H., Braude A. I., Wells W. W. Protection against lethality of E. coli endotoxin with "O" antiserum. Ann N Y Acad Sci. 1966 Jun 30;133(2):746–762. doi: 10.1111/j.1749-6632.1966.tb52403.x. [DOI] [PubMed] [Google Scholar]
  13. Ziegler E. J., Douglas H., Braude A. I. Experimental bacteremia due to Pseudomonas in agranulocytic animals. J Infect Dis. 1974 Nov;130 (Suppl)(0):S145–S148. doi: 10.1093/infdis/130.supplement.s145. [DOI] [PubMed] [Google Scholar]
  14. Ziegler E. J., Douglas H., Sherman J. E., Davis C. E., Braude A. I. Treatment of E. coli and klebsiella bacteremia in agranulocytic animals with antiserum to a UDP-gal epimerase-deficient mutant. J Immunol. 1973 Aug;111(2):433–438. [PubMed] [Google Scholar]
  15. Zinner S. H., McCabe W. R. Effects of IgM and IgG antibody in patients with bacteremia due to gram-negative bacilli. J Infect Dis. 1976 Jan;133(1):37–45. doi: 10.1093/infdis/133.1.37. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES