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. 1977 Nov;18(2):370–376. doi: 10.1128/iai.18.2.370-376.1977

Effect of interferon, elevated temperature, and cell type on replication of acute hemorrhagic conjunctivitis viruses.

G J Stanton, M P Langford, S Baron
PMCID: PMC421242  PMID: 200562

Abstract

Five strains of enterovirus type 70 (E 70) and four of coxsackievirus type A 24 (CA 24) were studied for their sensitivity to interferon (IF), ability to induce IF, replication at various temperatures, and adaptability to human and mouse cell cultures. We found that isolates ranged from 0.01 to 16 times as sensitive to fibroblast IF as vesicular stomatitis virus, depending upon the cell type used and the multiplicity of infection. Most of the isolates induced no detectable IF; however, when induction occurred the titers were relatively low (5 to 300 U). Only E 70 virus isolates were adaptable to growth in L-cells. Replication of all viruses was inhibited approximately 90% at 37 to 39 degrees C depending upon the cell type. These results and the accessibility of the eye to application of IF and/or heat suggests the possibility of their use for treatment. The adaptation of certain E 70 viruses to mouse L-cells opens the possibility of development of a mouse model infection.

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Selected References

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  1. Baker D. A., Glasgow L. A. Rapid plaque assay for encephalomyocarditis virus. Appl Microbiol. 1969 Nov;18(5):932–934. doi: 10.1128/am.18.5.932-934.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bharucha E. P., Mondkar V. P., Wardia N. H., Irani P. F., Katrak S. M. Neurological complications of a new conjunctivitis. Lancet. 1972 Nov 4;2(7784):970–971. doi: 10.1016/s0140-6736(72)92492-0. [DOI] [PubMed] [Google Scholar]
  3. Bharucha E. P., Mondkar V. P., Wardia N. H., Irani P. F., Katrak S. M. Neurological complications of a new conjunctivitis. Lancet. 1972 Nov 4;2(7784):970–971. doi: 10.1016/s0140-6736(72)92492-0. [DOI] [PubMed] [Google Scholar]
  4. Campbell J. B., Grunberger T., Kochman M. A., White S. L. A microplaque reduction assay for human and mouse interferon. Can J Microbiol. 1975 Aug;21(8):1247–1253. doi: 10.1139/m75-186. [DOI] [PubMed] [Google Scholar]
  5. Chatterjee S., Quarcoopome C. O., Apenteng A. Unusual type of epidemic conjunctivitis in Ghana. Br J Ophthalmol. 1970 Sep;54(9):628–630. doi: 10.1136/bjo.54.9.628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chomel J. J., Gaudin O. G., Nejmi S., Sohier R. Conjonctivite aiguë hémorragique épidémique due ă un entérovirus. Nouv Presse Med. 1973 Jun 30;2(26):1781–1783. [PubMed] [Google Scholar]
  7. Dumavibhat P., Panpatana P., Wasi C., Jatikavanij V., Sarasombath S., Thongcharoen P. An outbreak of acute hemorrhagic conjunctivitis in Thailand. I. Clinical observations. J Med Assoc Thai. 1973 May;56(5):267–272. [PubMed] [Google Scholar]
  8. Green J. A., Stanton G. J., Goode J., Baron S. Vesicular stomatitis virus plaque production in monolayer cultures with liquid overlay medium: description and adaptation to a one-day, human interferon-plaque. J Clin Microbiol. 1976 Dec;4(6):479–485. doi: 10.1128/jcm.4.6.479-485.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hierholzer J. C., Hilliard K. A., Esposito J. J. Serosurvey for "acute hemorrhagic conjunctivitis" virus (Enterovirus 70) antibodies in the Southeastern United States, with review of the literature and some epidemiologic implications. Am J Epidemiol. 1975 Dec;102(6):533–544. doi: 10.1093/oxfordjournals.aje.a112191. [DOI] [PubMed] [Google Scholar]
  10. Higgins P. G., Chapman T. E. Coxsackievirus A24 and acute haemorrhagic conjunctivitis in Sri Lanka. Lancet. 1977 Feb 12;1(8007):361–361. doi: 10.1016/s0140-6736(77)91159-x. [DOI] [PubMed] [Google Scholar]
  11. Hung T. P., Green I. J., Sung S. M. Neurologic complications with elevated antibody titer after acute hemorrhagic conjunctivitis. Am J Ophthalmol. 1975 Nov;80(5):832–834. doi: 10.1016/0002-9394(75)90279-2. [DOI] [PubMed] [Google Scholar]
  12. Jayavasu C., Santiswatdinont P., Srimarut S., Sangkawibha N. Characterization of a virus associated with epidemic conjunctivitis in Thailand. Biken J. 1975 Dec;18(4):249–255. [PubMed] [Google Scholar]
  13. Jones B. R. Epidemic haemorrhagic conjunctivitis in London 1971: a conjunctival picornavirus infection. Trans Ophthalmol Soc U K. 1972;92:625–627. [PubMed] [Google Scholar]
  14. Kono R. Apollo 11 disease or acute hemorrhagic conjunctivitis: a pandemic of a new enterovirus infection of the eyes. Am J Epidemiol. 1975 May;101(5):383–390. doi: 10.1093/oxfordjournals.aje.a112106. [DOI] [PubMed] [Google Scholar]
  15. Kono R., Miyamura K., Tajiri E., Robin Y., Girard P. Serological studies of radiculomyelitis occurring during the outbreak of acute hemorrhagic conjunctivitis in Senegal in 1970. Jpn J Med Sci Biol. 1976 Apr;29(2):91–94. doi: 10.7883/yoken1952.29.91. [DOI] [PubMed] [Google Scholar]
  16. Kono R., Miyamura K., Tajiri E., Shiga S., Sasagawa A., Irani P. F., Katrak S. M., Wadia N. H. Neurologic complications associated with acute hemorrhagic conjunctivitis virus infection and its serologic confirmation. J Infect Dis. 1974 May;129(5):590–593. doi: 10.1093/infdis/129.5.590. [DOI] [PubMed] [Google Scholar]
  17. Kono R., Sasagawa A., Kodama H., Uchida N., Akao Y., Mukoyama J., Fujiwara T. Neurovirulence of acute-haemorrhagic-conjunctivitis virus in monkeys. Lancet. 1973 Jan 13;1(7794):61–63. doi: 10.1016/s0140-6736(73)90464-9. [DOI] [PubMed] [Google Scholar]
  18. Kono R., Sasagawa A., Miyamura K., Tajiri E. Serologic characterization and sero-epidemiologic studies on acute hemorrhagic conjunctivitis (AHC) virus. Am J Epidemiol. 1975 May;101(5):444–457. doi: 10.1093/oxfordjournals.aje.a112112. [DOI] [PubMed] [Google Scholar]
  19. Kono R. [On the causative agent of acute haemorrhagic conjunctivitis and its epidemiology (author's transl)]. Nippon Ganka Gakkai Zasshi. 1974 Jul 10;78(7):333–341. [PubMed] [Google Scholar]
  20. Likar M., Talanyi-Pfeifer L., Marin J. An outbreak of acute hemorrhagic conjunctivitis in Yugoslavia in 1973. Pathol Microbiol (Basel) 1975;42(1):29–35. doi: 10.1159/000162718. [DOI] [PubMed] [Google Scholar]
  21. Maichuk Y. F. [Epidemic haemorrhagic conjunctivitis in the Middle East]. Rev Int Trach Pathol Ocul Trop Subtrop. 1975;52(1-2):107–122. [PubMed] [Google Scholar]
  22. Melnick J. L., Tagaya I., von Magnus H. Enteroviruses 69, 70, and 71. Intervirology. 1974;4(6):369–370. doi: 10.1159/000149872. [DOI] [PubMed] [Google Scholar]
  23. Mirkovic R. R., Schmidt N. J., Yin-Murphy M., Melnick J. L. Enterovirus etiology of the 1970 Singapore epidemic of acute conjunctivitis. Intervirology. 1974;4(2):119–127. doi: 10.1159/000149850. [DOI] [PubMed] [Google Scholar]
  24. Miyamura K., Yamazaki S., Tajiri E., Kono R. Growth characteristics of acute hemorrhagic conjunctivitis (AHC) virus in monkey kidney cells. I. Effect of temperature on viral growth. Intervirology. 1974;4(5):279–286. doi: 10.1159/000149860. [DOI] [PubMed] [Google Scholar]
  25. Phuapradit P., Roongwithu N., Limsukon P., Boongird P., Vejjajiva A. Radiculomyelitis complicating acute haemorrhagic conjunctivitis. A clinical study. J Neurol Sci. 1976 Jan;27(1):117–122. doi: 10.1016/0022-510x(76)90239-2. [DOI] [PubMed] [Google Scholar]
  26. Sasagawa A., Kono R., Konno K. Laboratory-acquired infection of the eye with AHC virus. Jpn J Med Sci Biol. 1976 Apr;29(2):95–97. doi: 10.7883/yoken1952.29.95. [DOI] [PubMed] [Google Scholar]
  27. Tilles J. G., Finland M. Microassay for human and chick cell interferons. Appl Microbiol. 1968 Nov;16(11):1706–1707. doi: 10.1128/am.16.11.1706-1707.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wadia N. H., Irani P. F., Katrak S. M. Lumbosacral radiculomyelitis associated with pandemic acute haemorrhagic conjunctivitis. Lancet. 1973 Feb 17;1(7799):350–352. doi: 10.1016/s0140-6736(73)90133-5. [DOI] [PubMed] [Google Scholar]
  29. Yang Y. F., Hung P. T., Lin L. K., Green I. J., Hung S. C. Epidemic hemorrhagic keratoconjunctivitis. Am J Ophthalmol. 1975 Aug;80(2):192–197. doi: 10.1016/0002-9394(75)90131-2. [DOI] [PubMed] [Google Scholar]
  30. Yin-Murphy M. An epidemic of picornavirus conjunctivitis in Singapore. Southeast Asian J Trop Med Public Health. 1972 Sep;3(3):303–309. [PubMed] [Google Scholar]
  31. Yin-Murphy M., Lim K. H., Ho Y. M. A coxsackievirus type A24 epidemic of acute conjunctivitis. Southeast Asian J Trop Med Public Health. 1976 Mar;(1):1–5. [PubMed] [Google Scholar]
  32. Yin-Murphy M. The picornaviruses of acute haemorrhagic conjunctivitis: a comparative study. Southeast Asian J Trop Med Public Health. 1973 Sep;4(3):305–310. [PubMed] [Google Scholar]
  33. Yin-Murphy M. Viruses of acute haemorrhagic conjunctivitis. Lancet. 1973 Mar 10;1(7802):545–546. doi: 10.1016/s0140-6736(73)90351-6. [DOI] [PubMed] [Google Scholar]

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