Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Dec;18(3):816–822. doi: 10.1128/iai.18.3.816-822.1977

Epidemic caprine keratoconjunctivitis: experimentally induced disease with a pure culture of Mycoplasma conjunctivae.

S L Trotter, R M Franklin, E J Baas, M F Barile
PMCID: PMC421306  PMID: 591067

Abstract

The induction of caprine keratoconjunctivitis by the subconjunctival inoculation of a cloned culture of Mycoplasma conjunctivae is described. The clinical course of the experimental disease was similar to that noted in naturally occurring outbreaks of "pink-eye" among goats, and biopsies of inflamed conjunctivae showed similar histological response. M. conjunctivae was consistently recovered from the inflamed conjunctival tissues of inoculated animals that developed ocular disease, thus fulfilling Koch's postulates and establishing this organism as an etiological agent of caprine keratoconjunctivitis. Immunological studies suggested that cellular immune mechanisms may play a role in protecting animals from disease produced by this mycoplasma.

Full text

PDF

Images in this article

819Image
on p.819
820Image
on p.820
821Image
on p.821

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baas E. J., Trotter S. L., Franklin R. M., Barile M. F. Epidemic caprine keratoconjunctivitis: recovery of Mycoplasma conjunctivae and its possible role in pathogenesis. Infect Immun. 1977 Dec;18(3):806–815. doi: 10.1128/iai.18.3.806-815.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barile M. F., Del Giudice R. A., Tully J. G. Isolation and characterization of Mycoplasma conjunctivae sp. n. from sheep and goats with keratoconjunctivitis. Infect Immun. 1972 Jan;5(1):70–76. doi: 10.1128/iai.5.1.70-76.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DAJANI A. S., CLYDE W. A., Jr, DENNY F. W. EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT). J Exp Med. 1965 Jun 1;121:1071–1086. doi: 10.1084/jem.121.6.1071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Denny F. W., Clyde W. A., Jr, Glezen W. P. Mycoplasma pneumoniae disease: clinical spectrum, pathophysiology, epidemiology, and control. J Infect Dis. 1971 Jan;123(1):74–92. doi: 10.1093/infdis/123.1.74. [DOI] [PubMed] [Google Scholar]
  5. Fernald G. W., Clyde W. A., Jr, Bienenstock J. Immunoglobulin-containing cells in lungs of hamsters infected with Mycoplasma pneumoniae. J Immunol. 1972 May;108(5):1400–1408. [PubMed] [Google Scholar]
  6. Fernald G. W. In vitro response of human lymphocytes to Mycoplasma pneumoniae. Infect Immun. 1972 Apr;5(4):552–558. doi: 10.1128/iai.5.4.552-558.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klingler K., Nicolet J., Schipper E. Neue Befunde über die Gemsblindheit. Schweiz Arch Tierheilkd. 1969 Oct;111(10):587–602. [PubMed] [Google Scholar]
  8. Langford E. V. Mycoplasma and associated bacteria isolated from ovine pink-eye. Can J Comp Med. 1971 Jan;35(1):18–21. [PMC free article] [PubMed] [Google Scholar]
  9. Nicolet J., Freundt E. A. Isolation of mycoplasma conjunctivae from Chamois and sheep affected with kerato-conjunctivitis. Zentralbl Veterinarmed B. 1975 Jun;22(4):302–307. doi: 10.1111/j.1439-0450.1975.tb00591.x. [DOI] [PubMed] [Google Scholar]
  10. Nicolet J., Wanner M., Sturzenegger N., Messerli J., de Meuron P. A. Die infektiöse Keratokonjunktivitis des Schafes. Mögliche ätiologische Rolle von Mykoplasmen. Schweiz Arch Tierheilkd. 1974 Oct;116(10):435–446. [PubMed] [Google Scholar]
  11. Surman P. G. Cytology of "pink-eye" of sheep, including a reference to trachoma of man, by employing acridine orange and iodine stains, and isolation of Mycoplasma agents from infected sheep eyes. Aust J Biol Sci. 1968 Jun;21(3):447–467. doi: 10.1071/bi9680447. [DOI] [PubMed] [Google Scholar]
  12. Surman P. G. Mycoplasma aetiology of keratoconjunctivitis ("pink-eye") in domestic ruminants. Aust J Exp Biol Med Sci. 1973 Oct;51(5):589–607. doi: 10.1038/icb.1973.56. [DOI] [PubMed] [Google Scholar]
  13. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Waldman R. H., Henney C. S. Cell-mediated immunity and antibody responses in the respiratory tract after local and systemic immunization. J Exp Med. 1971 Aug 1;134(2):482–494. doi: 10.1084/jem.134.2.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. al-Aubaidi J. M., Dardiri A. H., Muscoplatt C. C., McCauley E. H. Identification and characterization of Acholeplasma oculusi spec. nov. from the eyes of goats with keratoconjunctivitis. Cornell Vet. 1973 Jan;63(1):117–129. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES