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. 1977 Jan;15(1):26–33. doi: 10.1128/iai.15.1.26-33.1977

Mitogenic stimulation of murine spleen cells: relation to susceptibility to Salmonella infection.

N von Jeney, E Günther, K Jann
PMCID: PMC421325  PMID: 319060

Abstract

The screening of several inbred strains of mice suggested that the capacity of their spleen cells to respond to the mitogenic effect of lipopolysaccharide (LPS) of gram-negative bacteria was correlated with their resistance to intraperitoneal infection with Salmonella typhimurium. An infection of LPS into mice caused changes in the in vitro responsiveness of their spleen cells to the mitogenic effects of LPS and phytohemagglutinin. Pretreatment of mice with whole ultraviolet (UV)-killed bacteria led to a marked rise in the in vitro response of the spleen cells to UV-killed bacteria, but not to LPS or or phytohemagglutinin. This enhanced response to UV-killed bacteria was not specific for the O antigens of the bacteria.

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Selected References

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  1. Anderson L. E., McClure W. O. An improved scintillation cocktail of high-solubilizing power. Anal Biochem. 1973 Jan;51(1):173–179. doi: 10.1016/0003-2697(73)90465-x. [DOI] [PubMed] [Google Scholar]
  2. Andersson J., Melchers F., Galanos C., Lüderitz O. The mitogenic effect of lipopolysaccharide on bone marrow-derived mouse lymphocytes. Lipid A as the mitogenic part of the molecule. J Exp Med. 1973 Apr 1;137(4):943–953. doi: 10.1084/jem.137.4.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Andersson J., Möller G., Sjöberg O. Selective induction of DNA synthesis in T and B lymphocytes. Cell Immunol. 1972 Aug;4(4):381–393. doi: 10.1016/0008-8749(72)90040-8. [DOI] [PubMed] [Google Scholar]
  4. BOHME D. H., SCHNEIDER H. A., LEE J. M. Some physiopathological parameters of natural resistance to infection in murine salmonellosis. J Exp Med. 1959 Jul 1;110(1):9–26. doi: 10.1084/jem.110.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins F. M. Recall of immunity in mice vaccinated with Salmonella enteritidis or Salmonella typhimurium. J Bacteriol. 1968 Jun;95(6):2014–2021. doi: 10.1128/jb.95.6.2014-2021.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coutinho A., Gronowicz E. Genetical control of B-cell responses. III. Requirement for functional mitogenicity of the antigen in thymus-independent specific responses. J Exp Med. 1975 Apr 1;141(4):753–760. [PMC free article] [PubMed] [Google Scholar]
  8. Gery I., Krüger J., Spiesel S. Z. Stimulation of B-lymphocytes by endotoxin. Reactions of thymus-deprived mice and karyotypic analysis of dividing cells in mice bearing T 6 T 6 thymus grafts. J Immunol. 1972 Apr;108(4):1088–1091. [PubMed] [Google Scholar]
  9. Glode L. M., Scher I., Osborne B., Rosenstreich D. L. Cellular mechanism of endotoxin unresponsiveness in C3H/HeJ mice. J Immunol. 1976 Feb;116(2):454–461. [PubMed] [Google Scholar]
  10. Gröschel D., Paas C. M., Rosenberg B. S. Inherited resistance and mouse typhoid. I. Some factors which affect the survival of infected mice. J Reticuloendothel Soc. 1970 Apr;7(4):484–499. [PubMed] [Google Scholar]
  11. JENKIN C. R., ROWLEY D., AUZINS I. THE BASIS FOR IMMUNITY TO MOUSE TYPHOID. I. THE CARRIER STATE. Aust J Exp Biol Med Sci. 1964 Apr;42:215–228. doi: 10.1038/icb.1964.23. [DOI] [PubMed] [Google Scholar]
  12. LOEB T., ZINDER N. D. A bacteriophage containing RNA. Proc Natl Acad Sci U S A. 1961 Mar 15;47:282–289. doi: 10.1073/pnas.47.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lagrange P. H., Mackaness G. B., Miller T. E., Pardon P. Effects of bacterial lipopolysaccharide on the induction and expression of cell-mediated immunity. I. Depression of the afferent arc. J Immunol. 1975 Jan;114(1 Pt 2):442–446. [PubMed] [Google Scholar]
  14. Mackaness G. B., Blanden R. V., Collins F. M. Host-parasite relations in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):573–583. doi: 10.1084/jem.124.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maier T., Oels H. C. Role of the macrophage in natural resistance to salmonellosis in mice. Infect Immun. 1972 Oct;6(4):438–443. doi: 10.1128/iai.6.4.438-443.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McMaster P. D., Franzl R. E. The primary immune response in mice. II. Cellular responses of lymphoid tissue accompanying the enhancement or complete suppression of antibody formation by a bacterial endotoxin. J Exp Med. 1968 Jun 1;127(6):1109–1125. doi: 10.1084/jem.127.6.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Melchers F., Braun V., Galanos C. The lipoprotein of the outer membrane of Escherichia coli: a B-lymphocyte mitogen. J Exp Med. 1975 Aug 1;142(2):473–482. doi: 10.1084/jem.142.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. North R. J. Importance of thymus-derived lymphocytes in cell-mediated immunity to infection. Cell Immunol. 1973 Apr;7(1):166–176. doi: 10.1016/0008-8749(73)90193-7. [DOI] [PubMed] [Google Scholar]
  19. North R. J., Mackaness G. B. Immunological control of macrophage proliferation in vivo. Infect Immun. 1973 Jul;8(1):68–73. doi: 10.1128/iai.8.1.68-73.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Peavy D. L., Adler W. H., Smith R. T. The mitogenic effects of endotoxin and staphylococcal enterotoxin B on mouse spleen cells and human peripheral lymphocytes. J Immunol. 1970 Dec;105(6):1453–1458. [PubMed] [Google Scholar]
  21. Plant J., Glynn A. A. Natural resistance to Salmonella infection, delayed hypersensitivity and Ir genes in different strains of mice. Nature. 1974 Mar 22;248(446):345–347. doi: 10.1038/248345a0. [DOI] [PubMed] [Google Scholar]
  22. Robson H. G., Vas S. I. Resistance of inbred mice to Salmonella typhimurium. J Infect Dis. 1972 Oct;126(4):378–386. doi: 10.1093/infdis/126.4.378. [DOI] [PubMed] [Google Scholar]
  23. Rosenstreich D. L., Glode L. M. Difference in B cell mitogen responsiveness between closely related strains of mice. J Immunol. 1975 Sep;115(3):777–780. [PubMed] [Google Scholar]
  24. Rosenstreich D. L., Nowotny A., Chused T., Mergenhagen S. E. In vitro transformation of mouse bone-marrow-derived (B) lymphocytes induced by the lipid component of endotoxin. Infect Immun. 1973 Sep;8(3):406–411. doi: 10.1128/iai.8.3.406-411.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Skidmore B. J., Chiller J. M., Morrison D. C., Weigle W. O. Immunologic properties of bacterial lipopolysaccharide (LPS): correlation between the mitogenic, adjuvant, and immunogenic activities. J Immunol. 1975 Feb;114(2 Pt 2):770–775. [PubMed] [Google Scholar]
  26. Skidmore B. J., Morrison D. C., Chiller J. M., Weigle W. O. Immunologic properties of bacterial lipopolysaccharide (LPS). II. The unresponsiveness of C3H/HeJ Mouse spleen cells to LPS-induced mitogenesis is dependent on the method used to extract LPS. J Exp Med. 1975 Dec 1;142(6):1488–1508. doi: 10.1084/jem.142.6.1488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sultzer B. M. Genetic control of leucocyte responses to endotoxin. Nature. 1968 Sep 21;219(5160):1253–1254. doi: 10.1038/2191253a0. [DOI] [PubMed] [Google Scholar]
  28. Sultzer B. M., Nilsson B. S. PPD tuberculin--a B-cell mitogen. Nat New Biol. 1972 Dec 13;240(102):198–200. doi: 10.1038/newbio240198a0. [DOI] [PubMed] [Google Scholar]
  29. Watson J., Riblet R. Genetic control of responses to bacterial lipopolysaccharides in mice. I. Evidence for a single gene that influences mitogenic and immunogenic respones to lipopolysaccharides. J Exp Med. 1974 Nov 1;140(5):1147–1161. doi: 10.1084/jem.140.5.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]

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