Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Feb;15(2):402–409. doi: 10.1128/iai.15.2.402-409.1977

Persistence of cytomegalovirus in human lymphoblasts and peripheral leukocyte cultures.

S St Jeor, A Weisser
PMCID: PMC421382  PMID: 191399

Abstract

The in vitro susceptibility of human peripheral lymhpocytes and lymphoblastoid (F265) cells to infection by human cytomegalovirus was examined. Infection of these cell types with cytomegalovirus resulted in a persistent type of infection rather than the typical growth curve observed with permissive fibroblastic cells. When infection of peripheral lymphocytes was associated with a blastogenic response, the virus persisted for a longer time and at a higher titer than in cells in which a blastogenic response did not occur. Autoradiographic studies and infectious-center assays indicated that only a small number of cells, resembling lymphocytes, were involved in virus persistence. Whether or not the persistence of the virus indicates release of input virus or synthesis or new virus was not determined.

Full text

PDF
409

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D., Ely M., Steger L. Post-transfusion cytomegaloviremia and persistence of cytomegalovirus in blood. Infect Immun. 1971 Jan;3(1):159–163. doi: 10.1128/iai.3.1.159-163.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Caul E. O., Mott M. G., Clarke S. K., Perham T. G., Wilson R. S. Cytomegalovirus infections after open heart surgery. A prospective study. Lancet. 1971 Apr 17;1(7703):777–780. doi: 10.1016/s0140-6736(71)91216-5. [DOI] [PubMed] [Google Scholar]
  3. Fiala M., Payne J. E., Berne T. V., Moore T. C., Henle W., Montgomerie J. Z., Chatterjee S. N., Guze L. B. Epidemiology of cytomegalovirus infection after transplantation and immunosuppression. J Infect Dis. 1975 Oct;132(4):421–433. doi: 10.1093/infdis/132.4.421. [DOI] [PubMed] [Google Scholar]
  4. Green J. A., Baron S. 5-iododeoxyuridine potentiation of the replication in vitro of several unrelated RNA and DNA viruses. Science. 1975 Dec 12;190(4219):1099–1101. doi: 10.1126/science.1188388. [DOI] [PubMed] [Google Scholar]
  5. Hanshaw J. B. Congenital cytomegalovirus infection: a fifteen year perspective. J Infect Dis. 1971 May;123(5):555–561. doi: 10.1093/infdis/123.5.555. [DOI] [PubMed] [Google Scholar]
  6. Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. Stimulation of cellular DNA synthesis by human cytomegalovirus. J Virol. 1974 Feb;13(2):353–362. doi: 10.1128/jvi.13.2.353-362.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jerkofsky M., Rapp F. Stimulation of adenovirus replication in simian cells in the absence of a helper virus by pretreatment of the cells with iododeoxyuridine. J Virol. 1975 Feb;15(2):253–258. doi: 10.1128/jvi.15.2.253-258.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Joncas J. H., Menezes J., Huang E. S. Persistence of CMV genome in lymphoid cells after congenital infection. Nature. 1975 Dec 4;258(5534):432–434. doi: 10.1038/258432a0. [DOI] [PubMed] [Google Scholar]
  9. Lang D. J., Noren B. Cytomegaloviremia following congenital infection. J Pediatr. 1968 Dec;73(6):812–819. doi: 10.1016/s0022-3476(68)80233-1. [DOI] [PubMed] [Google Scholar]
  10. Mirkovic R., Werch J., South M. A., Benyesh-Melnick M. Incidence of cytomegaloviremia in blood-bank donors and in infants with congenital cytomegalic inclusion disease. Infect Immun. 1971 Jan;3(1):45–50. doi: 10.1128/iai.3.1.45-50.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Olding L. B., Jensen F. C., Oldstone M. B. Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction. J Exp Med. 1975 Mar 1;141(3):561–572. doi: 10.1084/jem.141.3.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Plummer G., Goodheart C. R. Growth of murine cytomegalovirus in a heterologous cell system and its enhancement by 5-iodo-2'-deoxyuridine. Infect Immun. 1974 Jul;10(1):251–256. doi: 10.1128/iai.10.1.251-256.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  14. St Jeor S., Rapp F. Cytomegalovirus replication in cells pretreated with 5-iodo-2'-deoxyuridine. J Virol. 1973 Jun;11(6):986–990. doi: 10.1128/jvi.11.6.986-990.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Staal S. P., Rowe W. P. Enhancement of adenovirus infection in WI-38 and AGMK cells by pretreatment of cells with 5-iododeoxyuridine. Virology. 1975 Apr;64(2):513–519. doi: 10.1016/0042-6822(75)90128-2. [DOI] [PubMed] [Google Scholar]
  16. Vonka V., Benyesh-Melnick M. Interactions of human cytomegalovirus with human fibroblasts. J Bacteriol. 1966 Jan;91(1):213–220. doi: 10.1128/jb.91.1.213-220.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weller T. H. The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. I. N Engl J Med. 1971 Jul 22;285(4):203–214. doi: 10.1056/NEJM197107222850406. [DOI] [PubMed] [Google Scholar]
  18. Weller T. H. The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. II. N Engl J Med. 1971 Jul 29;285(5):267–274. doi: 10.1056/NEJM197107292850507. [DOI] [PubMed] [Google Scholar]
  19. Wentworth B. B., French L. Plaque assay of cytomegalovirus strains of human origin. Proc Soc Exp Biol Med. 1970 Nov;135(2):253–258. doi: 10.3181/00379727-135-35031. [DOI] [PubMed] [Google Scholar]
  20. Wu B. C., Dowling J. N., Armstrong J. A., Ho M. Enhancement of mouse cytomegalovirus infection during host-versus-graft reaction. Science. 1975 Oct 3;190(4209):56–58. doi: 10.1126/science.170676. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES