Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Mar;15(3):720–725. doi: 10.1128/iai.15.3.720-725.1977

Infectious virus-antibody complexes of sindbis virus.

J Symington, A K McCann, M J Schlesinger
PMCID: PMC421429  PMID: 870428

Abstract

Infectious virus-antibody complexes were formed when Sindbis virus was reacted with antibodies raised against purified viral envelope glycoproteins E1 and E2 as well as against preparations of intact virus. Results from rate zonal centrifugation in sucrose gradients of the complex formed with anti-E1 sera showed this complex to be about the same size as virions. A test of virus neutralization, based on direct plaque assay, by antibodies raised in rabbits and mice given virus in complete Freund adjuvant indicated the presence of antibodies able to complex but not neutralize virus. Conditions were found in which most of the virus was complexed and protected fron neutralization, suggesting that these sera may contain a mixed population of antiviral antibodies with different specificities and different avidities.

Full text

PDF
721

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dalrymple J. M., Schlesinger S., Russell P. K. Antigenic characterization of two sindbis envelope glycoproteins separated by isoelectric focusing. Virology. 1976 Jan;69(1):93–103. doi: 10.1016/0042-6822(76)90197-5. [DOI] [PubMed] [Google Scholar]
  2. Dalrymple J. M., Teramoto A. Y., Cardiff R. D., Russell P. K. Radioimmune precipitation of group A arboviruses. J Immunol. 1972 Sep;109(3):426–433. [PubMed] [Google Scholar]
  3. Hahon N. Neutralization of residual infectivity of Venezuelan equine encephalomyelitis virus by anti-gamma globulin. J Gen Virol. 1970 Mar;6(3):361–372. doi: 10.1099/0022-1317-6-3-361. [DOI] [PubMed] [Google Scholar]
  4. Hunsmann G., Moennig V., Pister L., Seifert E., Schäfer W. Properties of mouse leukemia viruses. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunological, interfering and hemagglutinating capacities. Virology. 1974 Dec;62(2):307–318. doi: 10.1016/0042-6822(74)90394-8. [DOI] [PubMed] [Google Scholar]
  5. Ihle J. N., Jr, Hanna M. G., Schafer W., Hunsmann G., Bolognesi D. P., HUPER G. Polypeptides of mammalian oncornaviruses. III. Localization of p 15 and reactivity with natural antibody. Virology. 1975 Jan;63(1):60–67. doi: 10.1016/0042-6822(75)90370-0. [DOI] [PubMed] [Google Scholar]
  6. Karl S. C., Thormar H. Antibodies produced by rabbits immunized with visna virus. Infect Immun. 1971 Dec;4(6):715–719. doi: 10.1128/iai.4.6.715-719.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LAFFERTY K. J. THE INTERACTION BETWEEN VIRUS AND ANTIBODY. I. KINETIC STUDIES. Virology. 1963 Sep;21:61–75. doi: 10.1016/0042-6822(63)90305-2. [DOI] [PubMed] [Google Scholar]
  8. LAFFERTY K. J. THE INTERACTION BETWEEN VIRUS AND ANTIBODY. II. MECHANISM OF THE REACTION. Virology. 1963 Sep;21:76–99. doi: 10.1016/0042-6822(63)90306-4. [DOI] [PubMed] [Google Scholar]
  9. Lagwinska E., Stewart C. C., Adles C., Schlesinger S. Replication of lactic dehydrogenase virus and Sindbis virus in mouse peritoneal macrophages. Induction of interferon and phenotypic mixing. Virology. 1975 May;65(1):204–214. doi: 10.1016/0042-6822(75)90021-5. [DOI] [PubMed] [Google Scholar]
  10. Majer M., Lik F. Sensitization of influenza virus A2-Singapore by antineuraminidase. J Gen Virol. 1971 Nov;13(2):355–356. doi: 10.1099/0022-1317-13-2-355. [DOI] [PubMed] [Google Scholar]
  11. Majer M. Virus sensitization. Curr Top Microbiol Immunol. 1972;58:69–84. doi: 10.1007/978-3-642-65357-5_2. [DOI] [PubMed] [Google Scholar]
  12. PFEFFERKORN E. R., HUNTER H. S. PURIFICATION AND PARTIAL CHEMICAL ANALYSIS OF SINDBIS VIRUS. Virology. 1963 Jul;20:433–445. doi: 10.1016/0042-6822(63)90092-8. [DOI] [PubMed] [Google Scholar]
  13. Scheele C. M., Pfefferkorn E. R. Kinetics of incorporation of structural proteins into Sindbis virions. J Virol. 1969 Apr;3(4):369–375. doi: 10.1128/jvi.3.4.369-375.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schlesinger M. J. Formation of an infectious virus-antibody complex with Rous sarcoma virus and antibodies directed against the major virus glycoprotein. J Virol. 1976 Mar;17(3):1063–1067. doi: 10.1128/jvi.17.3.1063-1067.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schlesinger M. J., Schlesinger S., Burge B. W. Identification of a second glycoprotein in Sindbis virus. Virology. 1972 Feb;47(2):539–541. doi: 10.1016/0042-6822(72)90298-x. [DOI] [PubMed] [Google Scholar]
  16. Webster R. G., Laver W. G. Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus. J Immunol. 1967 Jul;99(1):49–55. [PubMed] [Google Scholar]
  17. Westaway E. G. The neutralization of arboviruses. I. Neutralization homologous virus-serum mixtures with two group B arboviruses. Virology. 1965 Aug;26(4):517–527. doi: 10.1016/0042-6822(65)90313-2. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES