Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Mar;15(3):883–889. doi: 10.1128/iai.15.3.883-889.1977

Solid-phase radioimmunoassay of human immunoglobulin M and immunoglobulin G antibodies against herpes simplex virus type 1 capsid, envelope, and excreted antigens.

K O Kalimo, R J Marttila, K Granfors, M K Viljanen
PMCID: PMC421456  PMID: 192678

Abstract

A solid-phase radioimmunoassay developed in our laboratory for detection of human viral immunoglobulin M (IgM) and IgG antibodies was applied to demonstrate human class-specific antibody response against capsid, envelope, and excreted antigens of herpes simplex virus type 1. In primary infections, a clear IgM and IgG antibody response was found predominantly against the envelope components, whereas the IgM and IgG antibodies to the capsid antigen appeared more slowly. Increasing IgG antibody titers to the excreted antigen were also found in primary infections, though appearing more slowly than antibodies to the other subunit antigens. The antibody response against capsid and envelope antigens was not type specific, whereas in primary infections IgG class antibodies against the excreted antigen showed distinct type specificity. In recurrent infections, no significant level of IgM class antibodies was demonstrated, but in the patients with a severe secondary herpes simplex virus infection a definite IgM class antibody response was found against the envelope antigen. In addition, during severe secondary infections the antibody response against the excreted antigen was enhanced. The host IgG antibody response in recurrent infections was directed against the envelope and excreted antigens, whereas the level of the capsid antibodies was relatively stable.

Full text

PDF
883

Images in this article

885Image
on p.885

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arstila P., Vuorimaa T., Kalimo K., Halonen P., Viljanen M., Granfors K., Toivanen P. A solid-phase radioimmunoassay for IgG and IgM antibodies against measles virus. J Gen Virol. 1977 Jan;34(1):167–176. doi: 10.1099/0022-1317-34-1-167. [DOI] [PubMed] [Google Scholar]
  2. Back A. F., Schmidt N. J. Reactivity of envelope, capsid, and soluble antigens of Herpesvirus hominis types 1 and 2 in the indirect hemagglutination test. Infect Immun. 1974 Jul;10(1):102–106. doi: 10.1128/iai.10.1.102-106.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Erickson J. S., Kaplan A. S. Synthesis of proteins in cells infected with herpesvirus. IX. Sulfated proteins. Virology. 1973 Sep;55(1):94–102. doi: 10.1016/s0042-6822(73)81011-6. [DOI] [PubMed] [Google Scholar]
  4. Ito M., Barron A. L. Surface antigen produced by herpes simplex virus (HSV). J Immunol. 1972 Mar;108(3):711–718. [PubMed] [Google Scholar]
  5. Kalimo K. O., Meurman O. H., Halonen P. E., Ziola B. R., Viljanen M. K., Granfors K., Toivanen P. Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies. J Clin Microbiol. 1976 Aug;4(2):117–123. doi: 10.1128/jcm.4.2.117-123.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaplan A. S., Erickson J. S., Ben-Porat T. Synthesis of proteins in cells infected with herpesvirus. X. Proteins excreted by cells infected with herpes simplex virus, types 1 and 2. Virology. 1975 Mar;64(1):132–143. doi: 10.1016/0042-6822(75)90085-9. [DOI] [PubMed] [Google Scholar]
  7. Martin M. L., Palmer E. L., Kissling R. E. Complement-fixing antigens of herpes simplex virus types 1 and 2: reactivity of capsid, envelope, and soluble antigens. Infect Immun. 1972 Feb;5(2):248–254. doi: 10.1128/iai.5.2.248-254.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schwartz J., Elizan T. S. Altered immunologic specificity of cells infected with Herpes simplex virus: recognition of human antiserum. Z Immunitatsforsch Exp Klin Immunol. 1975 Feb;148(4):291–298. [PubMed] [Google Scholar]
  9. Smith J. W., Lowry S. P., Melnick J. L., Rawls W. E. Antibodies to surface antigens of herpesvirus type 1- and type 2-infected cells among women with cervical cancer and control women. Infect Immun. 1972 Mar;5(3):305–310. doi: 10.1128/iai.5.3.305-310.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Viljanen M. K., Granfors K., Toivanen P. Radioimmunoassay of class-specific antibodies (RIACA): chicken antibodies to bovine serum albumin. Immunochemistry. 1975 Aug;12(8):699–705. doi: 10.1016/0019-2791(75)90218-9. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES