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. 1978 Jun;20(3):613–618. doi: 10.1128/iai.20.3.613-618.1978

Immediate cytotoxicity of Chlamydia trachomatis for mouse peritoneal macrophages.

C C Kuo
PMCID: PMC421902  PMID: 352950

Abstract

The toxicity of Chlamydia trachomatis was studied with mouse peritoneal macrophage culture. Inoculation of 30 inclusion-forming units of trachoma B/TW-5/OT organisms and 250 inclusion-forming units of lymphogranuloma venereum L2/434/Bu organisms per cell caused immediated toxicity, with the killing of 40 to 90% of the macrophages within 6 h after inoculation. Inhibition of phagocytosis by adsorption at 0 degrees C or by NaF pretreatment of macrophages prevented the toxicity, indicating that chlamydiae must be phagocytized to induce toxicity. Infectivity and toxicity could be dissociated, since ultraviolet-inactivated chlamydiae were still toxic. However, the toxicity was destroyed by heating the organisms at 56 degrees C for 10 min. Tetracycline, and antichlamydial drug, did not prevent toxicity, indicating that multiplication of the organisms was not required to induce toxicity. Toxicity was not prevented by treatment of macrophages with hydrocortisone. The toxicity of trachoma TW-5 was reduced by the rabbit immune serum of trachoma TW-5 but not by the rabbit immune serum of psittacosis meningopneumonitis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BELL S. D., Jr, SNYDER J. C., MURRAY E. S. Immunization of mice against toxic doses of homologous elementary bodies of trachoma. Science. 1959 Sep 11;130(3376):626–627. doi: 10.1126/science.130.3376.626. [DOI] [PubMed] [Google Scholar]
  2. Ignarro L. J. Preservation of structural integrity of liver lysosomes and membrane-stabilizing action of anti-inflammatory drugs, catecholamines and cyclic adenosine monophosphate in isotonic salt media. Biochem Pharmacol. 1973 Jun 1;22(11):1269–1282. doi: 10.1016/0006-2952(73)90301-8. [DOI] [PubMed] [Google Scholar]
  3. Kellogg K. R., Horoschak K. D., Moulder J. W. Toxicity of low and moderate multiplicities of Chlamydia psittaci for mouse fibroblasts (L cells). Infect Immun. 1977 Nov;18(2):531–541. doi: 10.1128/iai.18.2.531-541.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kordová N., Poffenroth L., Wilt J. C. Lysosomes and the "toxicity" of rickettsiales. 3. Response of L cells infected with egg-attenuated C. psittaci 6BC strain. Can J Microbiol. 1972 Aug;18(8):1343–1348. doi: 10.1139/m72-206. [DOI] [PubMed] [Google Scholar]
  5. Kordová N., Wilt J. C. Lysosomes and the "toxicity" of Rickettsiales. I. Cytochemical studies of macrophages inoculated in vitro with C. psittaci 6BC. Can J Microbiol. 1972 Apr;18(4):457–464. doi: 10.1139/m72-071. [DOI] [PubMed] [Google Scholar]
  6. Kuo C. C. Cultures of Chlamydia trachomatis in mouse peritoneal macrophages: factors affecting organism growth. Infect Immun. 1978 May;20(2):439–445. doi: 10.1128/iai.20.2.439-445.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuo C. C., Wang S. P., Grayston J. T. Antimicrobial activity of several antibiotics and a sulfonamide against Chlamydia trachomatis organisms in cell culture. Antimicrob Agents Chemother. 1977 Jul;12(1):80–83. doi: 10.1128/aac.12.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lawn A. M., Blyth W. A., Taverne J. Interactions of TRIC agents with macrophages and BHK-21 cells observed by electron microscopy. J Hyg (Lond) 1973 Sep;71(3):515–528. doi: 10.1017/s0022172400046507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lin H., Stewart C. C. Colony formation by mouse peritoneal exudate cells in vitro. Nat New Biol. 1973 Jun 6;243(127):176–177. doi: 10.1038/newbio243176a0. [DOI] [PubMed] [Google Scholar]
  10. Rabinovitch M., DeStefano M. J. Use of the local anesthetic lidocaine for cell harvesting and subcultivation. In Vitro. 1975 Nov-Dec;11(6):379–381. doi: 10.1007/BF02616374. [DOI] [PubMed] [Google Scholar]
  11. Taverne J., Blyth W. A., Ballard R. C. Interactions of TRIC agents with macrophages: effects on lysosomal enzymes of the cell. J Hyg (Lond) 1974 Apr;72(2):297–309. doi: 10.1017/s0022172400023512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WANG S. P., GRAYSTON J. T. CLASSIFICATION OF TRACHOMA VIRUS STRAINS BY PROTECTION OF MICE FROM TOXIC DEATH. J Immunol. 1963 Jun;90:849–856. [PubMed] [Google Scholar]
  13. Wessells N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamada K. Microfilaments in cellular and developmental processes. Science. 1971 Jan 15;171(3967):135–143. doi: 10.1126/science.171.3967.135. [DOI] [PubMed] [Google Scholar]

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