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. 1978 Aug;21(2):526–531. doi: 10.1128/iai.21.2.526-531.1978

Biological evaluation of a methanol-soluble, heat-stable Escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.

M N Burgess, R J Bywater, C M Cowley, N A Mullan, P M Newsome
PMCID: PMC422027  PMID: 357288

Abstract

Escherichia coli P16 was shown to produce two heat-stable toxins (ST) with differing biological activity. The toxins were separated by methanol extraction, and the first, STa, was methanol soluble, partially heat stable, active in neonatal piglets (1 to 3 days old) and infant mice, but inactive in weaned pigs (7 to 9 weeks old); the second, STb, was methanol insoluble, active in weaned pigs and rabbit ligated loops, but inactive in infant mice. It is therefore suggested that use of suckling mice as indicators of ST production will fail to identify certain ST-producing strains.

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Selected References

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  1. Bertschinger H. U., Moon H. W., Whipp S. C. Association of Escherichia coli with the small intestinal epithelium. II. Variations in association index and the relationship between association index and enterosorption in pigs. Infect Immun. 1972 Apr;5(4):606–611. doi: 10.1128/iai.5.4.606-611.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bywater R. J. Dialysis and ultrafiltration of heat-stable enterotoxin from Escherichia coli. J Med Microbiol. 1972 Aug;5(3):337–343. doi: 10.1099/00222615-5-3-337. [DOI] [PubMed] [Google Scholar]
  3. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  4. Dobrescu L., Huygelen C. Susceptibility of the mouse intestine to heat-stable enterotoxin produced by enteropathogenic Escherichia coli of porcine origin. Appl Microbiol. 1973 Sep;26(3):450–451. doi: 10.1128/am.26.3.450-451.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Echeverria P. D., Chang C. P., Smith D. Enterotoxigenicity and invasive capacity of "enteropathogenic" serotypes of Escherichia coli. J Pediatr. 1976 Jul;89(1):8–10. doi: 10.1016/s0022-3476(76)80917-1. [DOI] [PubMed] [Google Scholar]
  6. Etkin S., Gorbach S. L. Studies on enterotoxin from Escherichia coli associated with acute diarrhea in man. J Lab Clin Med. 1971 Jul;78(1):81–87. [PubMed] [Google Scholar]
  7. Evans D. G., Evans D. J., Jr, Pierce N. F. Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins of Escherichia coli. Infect Immun. 1973 Jun;7(6):873–880. doi: 10.1128/iai.7.6.873-880.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Giannella R. A. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976 Jul;14(1):95–99. doi: 10.1128/iai.14.1.95-99.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gross R. J., Scotland S. M., Rowe B. Enterotoxin testing of Escherichia coli causing epidemic infantile enteritis in the U.K. Lancet. 1976 Mar 20;1(7960):629–631. doi: 10.1016/s0140-6736(76)90429-3. [DOI] [PubMed] [Google Scholar]
  10. Guerrant R. L., Moore R. A., Kirschenfeld P. M., Sande M. A. Role of toxigenic and invasive bacteria in acute diarrhea of childhood. N Engl J Med. 1975 Sep 18;293(12):567–572. doi: 10.1056/NEJM197509182931201. [DOI] [PubMed] [Google Scholar]
  11. Gyles C. L., Barnum D. A. A heat-labile enterotoxin from strains of Eschericha coli enteropathogenic for pigs. J Infect Dis. 1969 Oct;120(4):419–426. doi: 10.1093/infdis/120.4.419. [DOI] [PubMed] [Google Scholar]
  12. Gyles C. L., Stevens J. B., Craven J. A. A study of Escherichia coli strains isolated from pigs with gastro-intestinal disease. Can J Comp Med. 1971 Jul;35(3):258–266. [PMC free article] [PubMed] [Google Scholar]
  13. Jacks T. M., Wu B. J. Biochemical properties of Escherichia coli low-molecular-weight, heat-stable enterotoxin. Infect Immun. 1974 Feb;9(2):342–347. doi: 10.1128/iai.9.2.342-347.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Klipstein F. A., Lee C. S., Engert R. F. Assay of Escherichia coli enterotoxins by in vivo perfusion in the rat jejunum. Infect Immun. 1976 Oct;14(4):1004–1010. doi: 10.1128/iai.14.4.1004-1010.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kohler E. M. Enterotoxic activity of filtrates of escherichia coli in young pigs. Am J Vet Res. 1968 Dec;29(12):2263–2274. [PubMed] [Google Scholar]
  16. Larivière S., Lallier R. Escherichia coli strains isolated from diarrheic piglets in the Province of Quebec. Can J Comp Med. 1976 Apr;40(2):190–197. [PMC free article] [PubMed] [Google Scholar]
  17. Mitchell I. D., Tame M. J., Kenworthy R. Separation and purification of enterotoxins from a strain Escherichia coli pathogenic for pigs. J Med Microbiol. 1974 Nov;7(4):439–450. doi: 10.1099/00222615-7-4-439. [DOI] [PubMed] [Google Scholar]
  18. Mitchell I. de G., Tame M. J., Kenworthy R. Conditions for the production of Escherichia coli enterotoxin in a defined medium. J Med Microbiol. 1974 Aug;7(3):395–400. doi: 10.1099/00222615-7-3-395. [DOI] [PubMed] [Google Scholar]
  19. Moon H. W., Whipp S. C. Development of resistance with age by swine intestine to effects of enteropathogenic Escherichia coli. J Infect Dis. 1970 Sep;122(3):220–223. doi: 10.1093/infdis/122.3.220. [DOI] [PubMed] [Google Scholar]
  20. Moon H. W., Whipp S. C., Engstrom G. W., Baetz A. L. Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine. J Infect Dis. 1970 Feb;121(2):182–187. doi: 10.1093/infdis/121.2.182. [DOI] [PubMed] [Google Scholar]
  21. Mullan N. A., Burgess M. N., Newsome P. M. Characterization of a partially purified methanol-soluble heat-stable Escherichia coli enterotoxin in infant mice. Infect Immun. 1978 Mar;19(3):779–784. doi: 10.1128/iai.19.3.779-784.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nalin D. R., McLaughlin J. C., Rahaman M., Yunus M., Curlin G. Enterotoxigenic Escherichia coli and idiopathic diarrhoea in Bangladesh. Lancet. 1975 Dec 6;2(7945):1116–1119. doi: 10.1016/s0140-6736(75)91005-3. [DOI] [PubMed] [Google Scholar]
  23. Pierce N. F., Wallace C. K. Stimulation of jejunal secretion by a crude Escherichia coli enterotixin. Gastroenterology. 1972 Sep;63(6):439–448. [PubMed] [Google Scholar]
  24. Raska K., Raskova H. Recognising epidemic strains of E. coli. Lancet. 1976 Jun 12;1(7972):1300–1300. doi: 10.1016/s0140-6736(76)91773-6. [DOI] [PubMed] [Google Scholar]
  25. Sack D. A., McLaughlin J. C., Sack R. B., Orskov F., Orskov I. Enterotoxigenic Escherichia coli isolated from patients at a hospital in Dacca. J Infect Dis. 1977 Feb;135(2):275–280. doi: 10.1093/infdis/135.2.275. [DOI] [PubMed] [Google Scholar]
  26. Sack D. A., Merson M. H., Wells J. G., Sack R. B., Morris G. K. Diarrhoea associated with heat-stable enterotoxin-producing strains of Escherichia coli. Lancet. 1975 Aug 9;2(7928):239–241. doi: 10.1016/s0140-6736(75)90958-7. [DOI] [PubMed] [Google Scholar]
  27. Sack R. B., Sack D. A., Mehlman I. J., Orskov F., Orskov I. Enterotoxigenic Escherichia coli isolated from food. J Infect Dis. 1977 Feb;135(2):313–317. doi: 10.1093/infdis/135.2.313. [DOI] [PubMed] [Google Scholar]
  28. Shore E. G., Dean A. G., Holik K. J., Davis B. R. Enterotoxin-producing Escherichia coli and diarrheal disease in adult travelers: a prospective study. J Infect Dis. 1974 May;129(5):577–582. doi: 10.1093/infdis/129.5.577. [DOI] [PubMed] [Google Scholar]
  29. Sivaswamy G., Gyles C. L. The prevalence of enterotoxigenic Escherichia coli in the feces of calves with diarrhea. Can J Comp Med. 1976 Jul;40(3):241–246. [PMC free article] [PubMed] [Google Scholar]
  30. Smith H. W., Halls S. Observations by the ligated intestinal segment and oral inoculation methods on Escherichia coli infections in pigs, calves, lambs and rabbits. J Pathol Bacteriol. 1967 Apr;93(2):499–529. doi: 10.1002/path.1700930211. [DOI] [PubMed] [Google Scholar]
  31. Smith H. W., Halls S. Studies on Escherichia coli enterotoxin. J Pathol Bacteriol. 1967 Apr;93(2):531–543. doi: 10.1002/path.1700930212. [DOI] [PubMed] [Google Scholar]
  32. Smith H. W., Linggood M. A. Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. J Med Microbiol. 1972 May;5(2):243–250. doi: 10.1099/00222615-5-2-243. [DOI] [PubMed] [Google Scholar]
  33. Whipp S. C., Moon H. W., Lyon N. C. Heat-stable Escherichia coli enterotoxin production in vivo. Infect Immun. 1975 Aug;12(2):240–244. doi: 10.1128/iai.12.2.240-244.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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