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. 1978 Dec;22(3):649–656. doi: 10.1128/iai.22.3.649-656.1978

Lymphocyte transformation test in leprosy: decreased lymphocyte reactivity to Mycobacterium leprae in lepromatous leprosy, with no evidence for a generalized impairment.

W R Faber, D L Leiker, I M Nengerman, W P Zeijlemaker, P T Schellekens
PMCID: PMC422209  PMID: 153335

Abstract

Untreated leprosy patients were examined with respect to lymphocyte transformation in vitro after stimulation with mycobacterial and other microbial antigens, allogeneic lymphocytes, or nonspecific mitogens. Methods were used to circumvent technical variability. The results were compared with those obtained in controls matched for age, sex, race, and environment. No evidence was found for a generalized impairment of lymphocyte transformation in vitro, whereas a specific defect towards Mycobacterium leprae was demonstrable in lepromatous leprosy patients. The response to M. leprae, investigated in untreated and treated leprosy patients, decreased along the leprosy spectrum. Moreover, the results of the one-way mixed lymphocyte cultures showed that lymphocytes from leprosy patients had a normal stimulator and responder capacity, when they were tested against a panel of allogeneic lymphocytes. The influence of serum factors was investigated in untreated leprosy patients in the mixed lymphocyte culture. On average, tuberculoid as well as lepromatous sera showed a low-level depressive effect, but some sera showed a stimulatory effect. Therefore, a depressive effect of serum factors cannot be considered to be a general feature of leprosy. The correlation between the Mitsuda type of lepromin skin test and the lymphocyte reactivity in vitro to M. leprae was studied, and a positive correlation was found.

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Selected References

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  1. BUCK A. A., HASENCLEVER H. F. The influence of leprosy on delayed-type skin reactions and serum agglutination titers to Candida albicans. A comparative study of patients with lepromatous and tuberculoid leprosy and controls in Ethiopia. Am J Hyg. 1963 May;77:305–316. doi: 10.1093/oxfordjournals.aje.a120321. [DOI] [PubMed] [Google Scholar]
  2. Barnetson R. S., Bjune G., Pearson J. M., Kronvall G. Antigenic heterogeneity in patients with reactions in borderline leprosy. Br Med J. 1975 Nov 22;4(5994):435–437. doi: 10.1136/bmj.4.5994.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bjune G., Barnetson R. S., Ridley D. S., Kronvall G. Lymphocyte transformation test in leprosy; correlation of the response with inflammation of lesions. Clin Exp Immunol. 1976 Jul;25(1):85–94. [PMC free article] [PubMed] [Google Scholar]
  4. Blanden R. V., Lefford M. J., Mackaness G. B. The host response to Calmette-Guérin bacillus infection in mice. J Exp Med. 1969 May 1;129(5):1079–1107. doi: 10.1084/jem.129.5.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bullock W. E., Jr, Fasal P. Studies of immune mechanisms in leprosy. 3. The role of cellular and humoral factors in impairment of the in vitro immune response. J Immunol. 1971 Apr;106(4):888–899. [PubMed] [Google Scholar]
  6. Bullock W. E., Jr Perturbation of lymphocyte circulation in experimental murine leprosy. I. Description of the defect. J Immunol. 1976 Oct;117(4):1164–1170. [PubMed] [Google Scholar]
  7. Closs O. Experimental murine leprosy: induction of immunity and immune paralysis to Mycobacterium lepraemurium in C57BL mice. Infect Immun. 1975 Oct;12(4):706–713. doi: 10.1128/iai.12.4.706-713.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Closs O., Haugen O. A. Experimental murine leprosy. 2. Further evidence for varying susceptibility of outbred mice and evaluation of the response of 5 inbred mouse strains to infection with Mycobacterium lepraemurium. Acta Pathol Microbiol Scand A. 1974 Jul;82(4):459–474. [PubMed] [Google Scholar]
  9. Du Bois M. J., Schellekens P. T., De Wit J. J., Eijsvoogel V. P. In vitro reactivity of human lymphocytes after cryopreservation using a programmed cooling device. Scand J Immunol. 1976 Jun;Suppl 5:17–22. doi: 10.1111/j.1365-3083.1976.tb03852.x. [DOI] [PubMed] [Google Scholar]
  10. Dwyer J. M., Bullock W. E., Fields J. P. Disturbance of the blood T:B lymphocyte ratio in lepromatous leprosy. Clinical and immunologic correlations. N Engl J Med. 1973 May 17;288(20):1036–1039. doi: 10.1056/NEJM197305172882002. [DOI] [PubMed] [Google Scholar]
  11. Godal T., Myklestad B., Samuel D. R., Myrvang B. Characterization of the cellular immune defect in lepromatous leprosy: a specific lack of circulating Mycobacterium leprae-reactive lymphocytes. Clin Exp Immunol. 1971 Dec;9(6):821–831. [PMC free article] [PubMed] [Google Scholar]
  12. Godal T., Myrvang B., Froland S. S., Shao J., Melaku G. Evidence that the mechanism of immunological tolerance ("central failure") is operative in the lack of host resistance in lepromatous leprosy. Scand J Immunol. 1972;1(4):311–321. doi: 10.1111/j.1365-3083.1972.tb03296.x. [DOI] [PubMed] [Google Scholar]
  13. Godal T., Myrvang B., Samuel D. R., Ross W. F., Lofgren M. Mechanism of "reactions" in borderline tuberculoid (BT) leprosy. A preliminary report. Acta Pathol Microbiol Scand Suppl. 1973;236(0):45–53. [PubMed] [Google Scholar]
  14. Godal T., Negassi K. Subclinical infection in leprosy. Br Med J. 1973 Sep 15;3(5880):557–559. doi: 10.1136/bmj.3.5880.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Han S. H., Weiser R. S., Kau S. T. Prolonged survival of skin allografts in leprosy patients. Int J Lepr Other Mycobact Dis. 1971 Jan-Mar;39(1):1–6. [PubMed] [Google Scholar]
  16. Han S. H., Weiser R. S., Lin Y. C. Transformation of leprous lymphocytes by leprolin, tuberculin and phytohemagglutinin. Int J Lepr Other Mycobact Dis. 1971 Oct-Dec;39(4):789–795. [PubMed] [Google Scholar]
  17. Hart P. D., Rees R. J. Lepromin and Kveim antigen reactivity in man, and their relation to tuberculin reactivity. Br Med Bull. 1967 Jan;23(1):80–85. doi: 10.1093/oxfordjournals.bmb.a070522. [DOI] [PubMed] [Google Scholar]
  18. Job C. K., Chacko C. J., Taylor P. M., Daniel M., Jesudian G. Evaluation of cell mediated immunity in the histopathologic spectrum of leprosy using lymphocyte transformation test. Int J Lepr Other Mycobact Dis. 1976 Jan-Jun;44(1-2):256–266. [PubMed] [Google Scholar]
  19. Leiker D. L. Classification of leprosy. Lepr Rev. 1966 Jan;37(1):7–15. doi: 10.5935/0305-7518.19660002. [DOI] [PubMed] [Google Scholar]
  20. Lim S. D., Jacobson R. R., Park B. H., Good R. A. Leprosy XII. Quantitative analysis of thymus-derived lymphocyte response to phytohemagglutinin in leprosy. Int J Lepr Other Mycobact Dis. 1975 Apr-Jun;43(2):95–100. [PubMed] [Google Scholar]
  21. Mehra V. L., Talwar G. P., Balakrishnan K., Bhutani L. K. Influence of chemotherapy and serum factors on the mitogenic response of peripheral leucocytes of leprosy patients to phytohaemagglutinin. Clin Exp Immunol. 1972 Oct;12(2):205–213. [PMC free article] [PubMed] [Google Scholar]
  22. Mendes E., Raphael A., Mota N. G., Mendes N. F. Cell-mediated immunity in leprosy and transfer of delayed hypersensitivity reactions. J Allergy Clin Immunol. 1974 Apr;53(4):223–229. doi: 10.1016/0091-6749(74)90084-0. [DOI] [PubMed] [Google Scholar]
  23. Myrvang B., Godal T., Ridley D. S., Fröland S. S., Song Y. K. Immune responsiveness to Mycobacterium leprae and other mycobacterial antigens throughout the clinical and histopathological spectrum of leprosy. Clin Exp Immunol. 1973 Aug;14(4):541–553. [PMC free article] [PubMed] [Google Scholar]
  24. Nath I., Curtis J., Sharma A. K., Talwar G. P. Circulating T-cell numbers and their mitogenic potential in leprosy--correlation with mycobacterial load. Clin Exp Immunol. 1977 Sep;29(3):393–400. [PMC free article] [PubMed] [Google Scholar]
  25. Nelson D. S., Penrose J. M., Waters M. F., Pearson J. M., Nelson M. Depressive effect of serum from patients with leprosy on mixed lymphocyte reactions. Influence of anti-leprosy treatment. Clin Exp Immunol. 1975 Dec;22(3):385–392. [PMC free article] [PubMed] [Google Scholar]
  26. Ptak W., Gaugas J. M., Rees R. J., Allison A. C. Immune responses in mice with murine leprosy. Clin Exp Immunol. 1970 Jan;6(1):117–124. [PMC free article] [PubMed] [Google Scholar]
  27. Rea T. H., Quismorio F. P., Harding B., Nies K. M., Di Saia P. J., Levan N. E., Friou G. J. Immunologic responses in patients with lepromatous leprosy. Arch Dermatol. 1976 Jun;112(6):791–800. [PubMed] [Google Scholar]
  28. Rea T. H., Quismorio F., Harding B., Friou G., Levan N. Quantitative dinitrochlorobenzene (DNCB) responsivity and phytohemagglutinin (PHA) induced lymphocyte transformation in patients with lepromatous leprosy. Int J Lepr Other Mycobact Dis. 1976 Jan-Jun;44(1-2):250–255. [PubMed] [Google Scholar]
  29. Ridley D. S., Jopling W. H. Classification of leprosy according to immunity. A five-group system. Int J Lepr Other Mycobact Dis. 1966 Jul-Sep;34(3):255–273. [PubMed] [Google Scholar]
  30. Ridley D. S. Reactions in leprosy. Lepr Rev. 1969 Apr;40(2):77–81. doi: 10.5935/0305-7518.19690016. [DOI] [PubMed] [Google Scholar]
  31. Rook G. A. The immunological consequences of antigen overload in experimental mycobacterial infections of mice. Clin Exp Immunol. 1975 Jan;19(1):167–177. [PMC free article] [PubMed] [Google Scholar]
  32. Saha K., Mittal M. M. A study of cell mediated immunity in leprosy: changing trends in the immunological spectrum of the disease. Clin Exp Immunol. 1971 Jun;8(6):901–909. [PMC free article] [PubMed] [Google Scholar]
  33. Saha K., Mittal M. M. Normal lymphocyte transfer tests in leprosy. Clin Exp Immunol. 1970 Jun;6(6):969–974. [PMC free article] [PubMed] [Google Scholar]
  34. Schwab J. H. Suppression of the immune response by microorganisms. Bacteriol Rev. 1975 Jun;39(2):121–143. doi: 10.1128/br.39.2.121-143.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Sheagren J. N., Block J. B., Trautman J. R., Wolff S. M. Immunologic reactivity in patients with leprosy. Ann Intern Med. 1969 Feb;70(2):295–302. doi: 10.7326/0003-4819-70-2-295. [DOI] [PubMed] [Google Scholar]
  36. Talwar G. P., Krishnan A. D., Mehra V. L., Blum E. A., Pearson J. M. Evaluation of cell mediated immune responses in untreated cases of leprosy. Clin Exp Immunol. 1972 Oct;12(2):195–203. [PMC free article] [PubMed] [Google Scholar]
  37. Turk J. L., Waters M. F. Cell-mediated immunity in patients with leprosy. Lancet. 1969 Aug 2;2(7614):243–246. doi: 10.1016/s0140-6736(69)90009-9. [DOI] [PubMed] [Google Scholar]
  38. Ulrich M., de Salas B., Convit J. Lymphocyte transformation with phytomitogens in leprosy. Int J Lepr Other Mycobact Dis. 1972 Jan-Mar;40(1):4–9. [PubMed] [Google Scholar]
  39. Waksman B. H. The early immune response to Mycobacterium lepraemurium in inbred mice and rats. Clin Immunol Immunopathol. 1973 Nov;2(1):82–97. doi: 10.1016/0090-1229(73)90039-1. [DOI] [PubMed] [Google Scholar]
  40. Waldorf D. S., Sheagren J. N., Trautman J. R., Block J. B. Impaired delayed hypersensitivity in patients with lepromatous leprosy. Lancet. 1966 Oct 8;2(7467):773–776. doi: 10.1016/s0140-6736(66)90366-7. [DOI] [PubMed] [Google Scholar]

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