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. 1978 Dec;22(3):861–866. doi: 10.1128/iai.22.3.861-866.1978

Proteolytic activity of Candida albicans: action on human salivary proteins.

G R Germaine, L M Tellefson, G L Johnson
PMCID: PMC422238  PMID: 32141

Abstract

The susceptibility of human salivary proteins to degradation by Candida albicans was studied. The organism was cultivated in either whole-salivary supernatant or parotid fluid, both of which were supplemented with glucose (0.1%). The culture pH's were at, or above, neutrality. After growth, the culture supernatant solutions were examined by polyacrylamide gel electrophoresis for alterations in their profiles of salivary proteins. No evidence of proteolysis of whole-saliva or parotid fluid proteins was found. Salivary proteins, however, are susceptible to degradation by preparations of C. albicans protease. Candida protease was incubated with parotid fluid adjusted to several pH values. After incubation the reaction mixtures were subjected to polyacrylamide gel electrophoresis. Extensive degradation of parotid proteins was found at pH 4, very slight proteolysis at pH 5, and no degradation at pH 6 or 7. No selectivity in proteolysis of the several parotid proteins was noted. These results indicate that C. albicans protease is strictly dependent upon low (ca. 4) pH for activity on salivary proteins. Furthermore, it is suggested that due to the pH requirements of the enzyme, it is unlikely to be of major significance to the pathogenesis of Candida-induced oral inflammatory lesions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Budtz-Jorgensen E., Bertram U. Denture stomatitis. I. The etiology in relation to trauma and infection. Acta Odontol Scand. 1970 Mar;28(1):71–92. doi: 10.3109/00016357009033133. [DOI] [PubMed] [Google Scholar]
  2. Budtz-Jorgensen E. Denture stomatitis. 3. Histopathology of trauma- and candida-induced inflammatory lesions of the palatal mucosa. Acta Odontol Scand. 1970 Nov;28(5):551–579. doi: 10.3109/00016357009058583. [DOI] [PubMed] [Google Scholar]
  3. Budtz-Jorgensen E. Denture stomatitis. IV. An experimental model in monkeys. Acta Odontol Scand. 1971 Nov;29(5):513–526. doi: 10.3109/00016357109026330. [DOI] [PubMed] [Google Scholar]
  4. Chattaway F. W., Odds F. C., Barlow A. J. An examination of the production of hydrolytic enzymes and toxins by pathogenic strains of Candida albicans. J Gen Microbiol. 1971 Aug;67(3):255–263. doi: 10.1099/00221287-67-3-255. [DOI] [PubMed] [Google Scholar]
  5. Evans E. G., Odds F. C., Richardson M. D., Holland K. T. The effect of growth medium of filament production in Candida albicans. Sabouraudia. 1974 Mar;12(1):112–119. doi: 10.1080/00362177485380151. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Liljemark W. F., Gibbons R. J. Suppression of Candida albicans by human oral streptococci in gnotobiotic mice. Infect Immun. 1973 Nov;8(5):846–849. doi: 10.1128/iai.8.5.846-849.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Olsen I., Birkeland J. M. Denture stomatitis-yeast occurrence and the pH of saliva and denture plaque. Scand J Dent Res. 1977 Jan-Feb;85(2):130–134. doi: 10.1111/j.1600-0722.1977.tb00543.x. [DOI] [PubMed] [Google Scholar]
  9. Olsen I., Haanaes H. R. Experimental palatal candidosis and saliva flow in monkeys. Scand J Dent Res. 1977 Jan-Feb;85(2):135–141. doi: 10.1111/j.1600-0722.1977.tb00544.x. [DOI] [PubMed] [Google Scholar]
  10. Plaut A. G., Wistar R., Jr, Capra J. D. Differential susceptibility of human IgA immunoglobulins to streptococcal IgA protease. J Clin Invest. 1974 Dec;54(6):1295–1300. doi: 10.1172/JCI107875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Remold H., Fasold H., Staib F. Purification and characterization of a proteolytic enzyme from Candida albicans. Biochim Biophys Acta. 1968 Oct 8;167(2):399–406. doi: 10.1016/0005-2744(68)90219-2. [DOI] [PubMed] [Google Scholar]
  12. Smith Q. T., Shapiro B. L., Hamilton M. J. Polyacrylamide gel patterns of parotid saliva proteins in Caucasoids and Amerindians. Arch Oral Biol. 1975 May-Jun;20(5-6):369–373. doi: 10.1016/0003-9969(75)90029-1. [DOI] [PubMed] [Google Scholar]

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