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. 1978 Mar;19(3):983–991. doi: 10.1128/iai.19.3.983-991.1978

Existence of multiple immunodeterminants in the type-specific capsular substance of group B type Ia streptococci.

J A Kane, W W Karakawa
PMCID: PMC422288  PMID: 76609

Abstract

Rabbits immunized with group B, type Ia streptococci produce two distinct populations of protective antibodies. Evidence is presented indicating that these antibodies are directed against two major immunodeterminants which coexist in the specific type Ia antigen. Immunochemical data, using purified antibody preparations, indicate that the type substance, a sialic acid polymer consisting of galactose, glucose, glucosamine, and sialic acid, possesses two distinct immunodominant determinants, terminal sialic acid residues and a galactosyl oligosaccharide. Antibodies directed against either of these determinants were shown to possess in vivo and in vitro opsonic capabilities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun D. G., Krause R. M. The individual antigenic specificity of antibodies to streptococcal carbohydrates. J Exp Med. 1968 Nov 1;128(5):969–989. doi: 10.1084/jem.128.5.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Freimer E. H. Type-specific polysaccharide antigens of group B streptococci. II. The chemical basis for serological specificity of the type II HCl antigen. J Exp Med. 1967 Mar 1;125(3):381–392. doi: 10.1084/jem.125.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KRAUSE R. M. SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. IV. ANTIGENIC AND BIOCHEMICAL COMPOSITION OF HEMOLYTIC STREPTOCOCCAL CELL WALLS. Bacteriol Rev. 1963 Dec;27:369–380. doi: 10.1128/br.27.4.369-380.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kane J. A., Karakawa W. W. Multiple polysaccharide antigens of group B streptococcus, type Ia: emphasis on a sialic acid type-specific polysaccharide. J Immunol. 1977 Jun;118(6):2155–2160. [PubMed] [Google Scholar]
  5. Kane J. A., Karakawa W. W., Pazur J. H. Glycans from streptococcal cell walls: structural features of a diheteroglycan isolated from the cell wall of Streptococcus bovis. J Immunol. 1972 May;108(5):1218–1226. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lancefield R. C., Freimer E. H. Type-specific polysaccharide antigens of group B streptococci. J Hyg (Lond) 1966 Jun;64(2):191–203. doi: 10.1017/s0022172400040456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lancefield R. C., McCarty M., Everly W. N. Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens. J Exp Med. 1975 Jul 1;142(1):165–179. doi: 10.1084/jem.142.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MCCARTY M. THE ROLE OF D-ALANINE IN THE SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL GLYCEROL TEICHOIC ACID. Proc Natl Acad Sci U S A. 1964 Aug;52:259–265. doi: 10.1073/pnas.52.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCARTY M., LANCEFIELD R. C. Variation in the group-specific carbohydrate of group A streptococci. I. Immunochemical studies on the carbohydrates of variant strains. J Exp Med. 1955 Jul 1;102(1):11–28. doi: 10.1084/jem.102.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Partridge S. M. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948;42(2):238–250. doi: 10.1042/bj0420238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ROTH H., SEGAL S., BERTOLI D. THE QUANTITATIVE DETERMINATION OF GALACTOSE--AN ENZYMIC METHOD USING GALACTOSE OXIDASE, WITH APPLICATIONS TO BLOOD AND OTHER BIOLOGICAL FLUIDS. Anal Biochem. 1965 Jan;10:32–52. doi: 10.1016/0003-2697(65)90238-1. [DOI] [PubMed] [Google Scholar]
  13. Roberts R. B. The relationship between group A and group C meningococcal polysaccharides and serum opsonins in man. J Exp Med. 1970 Mar 1;131(3):499–513. doi: 10.1084/jem.131.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  15. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  16. Weber P., Winzler R. J. Determination of hexosaminitols by ion-exchange chromatography and its application to alkali-labile glycosidic linkages in glycoproteins. Arch Biochem Biophys. 1969 Feb;129(2):534–538. doi: 10.1016/0003-9861(69)90211-2. [DOI] [PubMed] [Google Scholar]
  17. Wilkinson H. W., Eagon R. G. Type-specific antigens of group B type Ic streptococci. Infect Immun. 1971 Nov;4(5):596–604. doi: 10.1128/iai.4.5.596-604.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wilkinson H. W. Immunochemistry of purified polysaccharide type antigens of group B streptococcal types Ia, Ib, and Ic. Infect Immun. 1975 Apr;11(4):845–852. doi: 10.1128/iai.11.4.845-852.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wilkinson H. W., Moody M. D. Serological relationships of type I antigens of group B streptococci. J Bacteriol. 1969 Feb;97(2):629–634. doi: 10.1128/jb.97.2.629-634.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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