Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1978 Mar;19(3):1097–1098. doi: 10.1128/iai.19.3.1097-1098.1978

Isoelectric point of cell-free K99 antigen exhibiting hemagglutinating properties.

J A Morris, A E Stevens, W J Sojka
PMCID: PMC422302  PMID: 346483

Abstract

The isoelectric point of the K99 antigen in partially purified preparations isolated from Escherichia coli B41 was 4.2. Electrofocused K99 antigen hemagglutinated guinea pig and sheep erythrocytes and gave a single precipitin line on diffusion against antisera to E. coli B41 and absorbed factor K99 antisera.

Full text

PDF
1097

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burrows M. R., Sellwood R., Gibbons R. A. Haemagglutinating and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli. J Gen Microbiol. 1976 Oct;96(2):269–275. doi: 10.1099/00221287-96-2-269. [DOI] [PubMed] [Google Scholar]
  2. Isaacson R. E. K99 surface antigen of Escherichia coli: purification and partial characterization. Infect Immun. 1977 Jan;15(1):272–279. doi: 10.1128/iai.15.1.272-279.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Moon H. W., Whipp S. C., Skartvedt S. M. Etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and K99 antigen production by Escherichia cola. Am J Vet Res. 1976 Sep;37(9):1025–1029. [PubMed] [Google Scholar]
  4. Morris J. A., Stevens A. E., Sojka W. J. Preliminary characterization of cell-free K99 antigen isolated from Escherichia coli B41. J Gen Microbiol. 1977 Apr;99(2):353–357. doi: 10.1099/00221287-99-2-353. [DOI] [PubMed] [Google Scholar]
  5. Myers L. L., Guinée P. A. Occurrence and characteristics of enterotoxigenic Escherichia coli isolated from calves with diarrhea. Infect Immun. 1976 Apr;13(4):1117–1119. doi: 10.1128/iai.13.4.1117-1119.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Orskov I., Orskov F., Smith H. W., Sojka W. J. The establishment of K99, a thermolabile, transmissible escherichia coli K antigen, previously called "Kco", possessed by calf and lamb enteropathogenic strains. Acta Pathol Microbiol Scand B. 1975 Feb;83(1):31–36. doi: 10.1111/j.1699-0463.1975.tb00066.x. [DOI] [PubMed] [Google Scholar]
  7. Smith H. W., Linggood M. A. Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. J Med Microbiol. 1972 May;5(2):243–250. doi: 10.1099/00222615-5-2-243. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES