Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1972 Jan;5(1):70–76. doi: 10.1128/iai.5.1.70-76.1972

Isolation and Characterization of Mycoplasma conjunctivae sp. n. from Sheep and Goats with Keratoconjunctivitis

Michael F Barile 1,2, Richard A Del Giudice 1,2, Joseph G Tully 1,2
PMCID: PMC422323  PMID: 4348078

Abstract

Fourteen conjunctival strains of mycoplasma were isolated from infected sheep and goats in two separate naturally occurring outbreaks of keratoconjunctivitis (pink-eye). The biological and serological properties and cell protein patterns of these strains are presented in this report. These strains were related to each other and were unrelated to 40 recognized Mycoplasma and Acholeplasma species and serotypes. We are proposing that this group of mycoplasmas be named M. conjunctivae. The role of M. conjunctivae in the etiology of keratoconjunctivitis in sheep and goats remains to be determined.

Full text

PDF
76

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARILE M. F., YAGUCHI R., EVELAND W. C. A simplified medium for the cultivation of pleuropneumonia-like organisms and the L-forms of bacteria. Am J Clin Pathol. 1958 Aug;30(2):171–176. doi: 10.1093/ajcp/30.2_ts.171. [DOI] [PubMed] [Google Scholar]
  2. Barile M. F., DelGiudice R. A., Carski T. R., Gibbs C. J., Morris J. A. Isolation and characterization of Mycoplasma arginini: spec. nov. Proc Soc Exp Biol Med. 1968 Nov;129(2):489–494. doi: 10.3181/00379727-129-33351. [DOI] [PubMed] [Google Scholar]
  3. CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
  4. CORDY D. R., ADLER H. E., YAMAMOTO R. A pathogenic pleuropneumonialike organism from goats. Cornell Vet. 1955 Jan;45(1):50–68. [PubMed] [Google Scholar]
  5. Del Giudice R. A., Robillard N. F., Carski T. R. Immunofluorescence identification of Mycoplasma on agar by use of incident illumination. J Bacteriol. 1967 Apr;93(4):1205–1209. doi: 10.1128/jb.93.4.1205-1209.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gourlay R. N., Thomas L. H. The islation of large colony and T-strain mycoplasmas from cases of bovine kerato-conjunctivitis. Vet Rec. 1969 Apr 19;84(16):416–417. doi: 10.1136/vr.84.16.416. [DOI] [PubMed] [Google Scholar]
  7. Jonas A. M., Barber T. L. Mycoplasma mycoides var, capri isolated from a goat in Connecticut. J Infect Dis. 1969 Feb;119(2):126–131. doi: 10.1093/infdis/119.2.126. [DOI] [PubMed] [Google Scholar]
  8. Langford E. V., Dorward W. J. A mycoplasma isolated from cattle with infectious bovine keratoconjunctivitis. Can J Comp Med. 1969 Oct;33(4):275–279. [PMC free article] [PubMed] [Google Scholar]
  9. Langford E. V. Mycoplasma and associated bacteria isolated from ovine pink-eye. Can J Comp Med. 1971 Jan;35(1):18–21. [PMC free article] [PubMed] [Google Scholar]
  10. Leach R. H. The occurrence of Mycoplasma arginini in several animal hosts. Vet Rec. 1970 Sep 12;87(11):319–320. doi: 10.1136/vr.87.11.319. [DOI] [PubMed] [Google Scholar]
  11. Lemcke R. M. Sizing small organisms. Nature. 1971 Feb 12;229(5285):492–493. doi: 10.1038/229492a0. [DOI] [PubMed] [Google Scholar]
  12. Manchee R. J., Taylor-Robinson D. Haemadsorption and haemagglutination by mycoplasmas. J Gen Microbiol. 1968 Mar;50(3):465–478. doi: 10.1099/00221287-50-3-465. [DOI] [PubMed] [Google Scholar]
  13. McCauley E. H., Surman P. G., Anderson D. R. Isolation of mycoplasma from goats during an epizootic of keratoconjunctivitis. Am J Vet Res. 1971 Jun;32(6):861–passim. [PubMed] [Google Scholar]
  14. Razin S. Mycoplasma taxonomy studiedy electrophoresis of cell proteins. J Bacteriol. 1968 Sep;96(3):687–694. doi: 10.1128/jb.96.3.687-694.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Razin S., Rottem S. Identification of Mycoplasma and other microorganisms by polyacrylamide-gel electrophoresis of cell proteins. J Bacteriol. 1967 Dec;94(6):1807–1810. doi: 10.1128/jb.94.6.1807-1810.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Razin S., Tully J. G. Cholesterol requirement of mycoplasmas. J Bacteriol. 1970 May;102(2):306–310. doi: 10.1128/jb.102.2.306-310.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rottem S., Razin S. Electrophoretic patterns of membrane proteins of Mycoplasma. J Bacteriol. 1967 Aug;94(2):359–364. doi: 10.1128/jb.94.2.359-364.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SOMERSON N. L., PURCELL R. H., TAYLOR-ROBINSON D., CHANOCK R. M. HEMOLYSIN OF MYCOPLASMA PNEUMONIAE. J Bacteriol. 1965 Mar;89:813–818. doi: 10.1128/jb.89.3.813-818.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Surman P. G. Cytology of "pink-eye" of sheep, including a reference to trachoma of man, by employing acridine orange and iodine stains, and isolation of Mycoplasma agents from infected sheep eyes. Aust J Biol Sci. 1968 Jun;21(3):447–467. doi: 10.1071/bi9680447. [DOI] [PubMed] [Google Scholar]
  20. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tully J. G., Barile M. F., Del Giudice R. A., Carski T. R., Armstrong D., Razin S. Proposal for classifying strain PG-24 and related canine mycoplasmas as Mycoplasma edwardii sp. n. J Bacteriol. 1970 Feb;101(2):346–349. doi: 10.1128/jb.101.2.346-349.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tully J. G., Razin S. Physiological and serological comparisons among strains of Mycoplasma granularum and Mycoplasma laidlawii. J Bacteriol. 1968 May;95(5):1504–1512. doi: 10.1128/jb.95.5.1504-1512.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES