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. 1972 Feb;5(2):213–221. doi: 10.1128/iai.5.2.213-221.1972

Production of Potent Anti-Australia Antigen Sera of High Specificity and Sensitivity in Goats

Gordon R Dreesman 1, F Blaine Hollinger 1, Robert M McCombs 1, Joseph L Melnick 1
PMCID: PMC422350  PMID: 4635499

Abstract

Potent antisera of high specificity and sensitivity were produced, in goats, to purified Australia antigen (Au). The antigen was prepared by one of three methods: (i) pelleting, low pH treatment, isopycnic centrifugation two times in CsCl, and rate zonal centrifugation in sucrose; (ii) same as procedure i, with the exception of the low pH treatment; or (iii) twice banding in CsCl by using a BXIV batch-type zonal centrifuge rotor with subsequent preparative Pevikon electrophoresis. The goat anti-Au sera contained high levels of precipitating antibody as tested by immunodiffusion in agar gel and discontinuous counterimmunoelectrophoresis (DCIE) as well as specific complement-fixing antibody and could be used for routine screening of sera for Au without prior adsorption with Au-negative normal human serum (NHS). Identification of 66 of 70 positive specimens (94.3%) in a panel of 98 coded sera (49 duplicates) with 100% reproducibility was made by using one of the goat anti-Au sera at a dilution of 1:16 in the DCIE method. No false positives were recorded. Low levels of antibody against NHS components were effectively removed by a single adsorption with glutaraldehyde cross-linked NHS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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