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. 1972 Apr;5(4):595–605. doi: 10.1128/iai.5.4.595-605.1972

Association of Escherichia coli with the Small Intestinal Epithelium I. Comparison of Enteropathogenic and Nonenteropathogenic Porcine Strains in Pigs

Hans U Bertschinger a,1, Harley W Moon a, Shannon C Whipp a
PMCID: PMC422410  PMID: 4564680

Abstract

Two enteropathogenic strains of Escherichia coli (EEC) differed from a nonenteropathogenic strain of E. coli (NEEC) in their association with porcine small intestinal epithelium. The EEC characteristically were found along villi from tip to base and contiguous to the brush border. They were not in crypts. In contrast, the NEEC characteristically remained in the central lumen near the tips of villi and was only occasionally contiguous to the brush border. No organisms were detected within epithelial cells. The difference in distribution between EEC and NEEC was apparent in ligated jejunal loops 45 min postexposure. The association between host and bacterial cells was most consistently demonstrated on frozen sections of intestine, as other histological techniques removed many bacteria. However, cellular details of the association were best demonstrated in chemically fixed tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arbuckle J. B. The location of Escherichia coli in the pig intestine. J Med Microbiol. 1970 May;3(2):333–340. doi: 10.1099/00222615-3-2-333. [DOI] [PubMed] [Google Scholar]
  2. Drees D. T., Waxler D. L. Enteric colibacillosis in gnotobiotic swine: a fluorescence microscopic study. Am J Vet Res. 1970 Jul;31(7):1147–1157. [PubMed] [Google Scholar]
  3. Drees D. T., Waxler G. L. Enteric colibacillosis in gnotobiotic swine: an electron microscopic study. Am J Vet Res. 1970 Jul;31(7):1159–1171. [PubMed] [Google Scholar]
  4. Drucker M. M., Yeivin R., Sacks T. G. Pathogenesis of Escherichia coli enteritis in the ligated rabbit gut. Isr J Med Sci. 1967 May-Jun;3(3):445–452. [PubMed] [Google Scholar]
  5. Gibbons R. J., van Houte J. Selective bacterial adherence to oral epithelial surfaces and its role as an ecological determinant. Infect Immun. 1971 Apr;3(4):567–573. doi: 10.1128/iai.3.4.567-573.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kenworthy R. Effect of Escherichia coli on germ-free and gnotobiotic pigs. I. Light and electron microscopy of the small intestine. J Comp Pathol. 1970 Jan;80(1):53–63. doi: 10.1016/0021-9975(70)90031-9. [DOI] [PubMed] [Google Scholar]
  7. Moon H. W., Whipp S. C., Baetz A. L. Comparative effects of enterotoxins from Escherichia coli and Vibrio cholerae on rabbit and swine small intestine. Lab Invest. 1971 Aug;25(2):133–140. [PubMed] [Google Scholar]
  8. Nelson D. P., Mata L. J. Bacterial flora associated with the human gastrointestinal mucosa. Gastroenterology. 1970 Jan;58(1):56–61. [PubMed] [Google Scholar]
  9. Neter E. Enteritis due to enteropathogenic Escherichia coli. Am J Dig Dis. 1965 Oct;10(10):883–886. doi: 10.1007/BF02236098. [DOI] [PubMed] [Google Scholar]
  10. SMITH H. W., JONES J. E. OBSERVATIONS ON THE ALIMENTARY TRACT AND ITS BACTERIAL FLORA IN HEALTHY AND DISEASED PIGS. J Pathol Bacteriol. 1963 Oct;86:387–412. [PubMed] [Google Scholar]
  11. Savage D. C., Dubos R., Schaedler R. W. The gastrointestinal epithelium and its autochthonous bacterial flora. J Exp Med. 1968 Jan 1;127(1):67–76. doi: 10.1084/jem.127.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith H. W., Halls S. Observations by the ligated intestinal segment and oral inoculation methods on Escherichia coli infections in pigs, calves, lambs and rabbits. J Pathol Bacteriol. 1967 Apr;93(2):499–529. doi: 10.1002/path.1700930211. [DOI] [PubMed] [Google Scholar]
  13. Smith H. W., Halls S. The production of oedema disease and diarrhoea in weaned pigs by the oral administration of Escherichia coli: factors that influence the course of the experimental disease. J Med Microbiol. 1968 Aug;1(1):45–59. doi: 10.1099/00222615-1-1-45. [DOI] [PubMed] [Google Scholar]
  14. Staley T. E., Jones E. W., Corley L. D. Attachment and penetration of Escherichia coli into intestinal epithelium of the ileum in newborn pigs. Am J Pathol. 1969 Sep;56(3):371–392. [PMC free article] [PubMed] [Google Scholar]
  15. TAYLOR J., MALTBY M. P., PAYNE J. M. Factors influencing the response of ligated rabbit-gut segments to injected Escherichia coli. J Pathol Bacteriol. 1958 Oct;76(2):491–499. doi: 10.1002/path.1700760218. [DOI] [PubMed] [Google Scholar]
  16. THOMASON B. M., CHERRY W. B., DAVIS B. R., POMALES-LEBRON A. Rapid presumptive identification of entero-pathogenic Escherichia coli in faecal smears by means of fluorescent antibody. 1. Preparation and testing of reagents. Bull World Health Organ. 1961;25:137–152. [PMC free article] [PubMed] [Google Scholar]

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