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. 1972 Apr;5(4):632–633. doi: 10.1128/iai.5.4.632-633.1972

Amino Acid Oxidase in Leukocytes: Evidence Against a Major Role in Phagocytosis

Lawrence R DeChatelet 1, Charles E McCall 1, M Robert Cooper 1
PMCID: PMC422417  PMID: 4404631

Abstract

The addition of either d- or l-amino acids fails to increase hexose monophosphate shunt activity of resting or phagocytizing neutrophils. This is presumptive evidence against a major role for amino acid oxidase in the bactericidal activity of the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CAGAN R. H., KARNOVSKY M. L. ENZYMATIC BASIS OF THE RESPIRATORY STIMULATION DURING PHAGOCYTOSIS. Nature. 1964 Oct 17;204:255–257. doi: 10.1038/204255a0. [DOI] [PubMed] [Google Scholar]
  2. Cline M. J., Lehrer R. I. D-amino acid oxidase in leukocytes: a possible D-amino-acid-linked antimicrobial system. Proc Natl Acad Sci U S A. 1969 Mar;62(3):756–763. doi: 10.1073/pnas.62.3.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooper M. R., McCall C. E., Dechatelet L. R. Stimulation of leukocyte hexose monophosphate shunt activity by ascorbic Acid. Infect Immun. 1971 Jun;3(6):851–853. doi: 10.1128/iai.3.6.851-853.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeChatelet L. R., Cooper M. R. A modified procedure for the determination of leukocyte alkaline phosphatase. Biochem Med. 1970 Aug;4(1):61–68. doi: 10.1016/0006-2944(70)90103-1. [DOI] [PubMed] [Google Scholar]
  5. Eckstein M. R., Baehner R. L., Nathan D. G. Amino acid oxidase of leukocytes in relation to H 2 O 2 -mediated bacterial killing. J Clin Invest. 1971 Sep;50(9):1985–1991. doi: 10.1172/JCI106690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holmes B., Page A. R., Good R. A. Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. J Clin Invest. 1967 Sep;46(9):1422–1432. doi: 10.1172/JCI105634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. IYER G. Y., QUESTEL J. H. NADPH and NADH oxidation by guinea pig polymorphonuclear leucocytes. Can J Biochem Physiol. 1963 Feb;41:427–434. [PubMed] [Google Scholar]
  8. KARNOVSKY M. L. Metabolic basis of phagocytic activity. Physiol Rev. 1962 Jan;42:143–168. doi: 10.1152/physrev.1962.42.1.143. [DOI] [PubMed] [Google Scholar]
  9. Klebanoff S. J. Iodination of bacteria: a bactericidal mechanism. J Exp Med. 1967 Dec 1;126(6):1063–1078. doi: 10.1084/jem.126.6.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller T. E. Killing and lysis of gram-negative bacteria through the synergistic effect of hydrogen peroxide, ascorbic acid, and lysozyme. J Bacteriol. 1969 Jun;98(3):949–955. doi: 10.1128/jb.98.3.949-955.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nathan D. G., Baehner R. L., Weaver D. K. Failure of nitro blue tetrazolium reduction in the phagocytic vacuoles of leukocytes in chronic granulomatous disease. J Clin Invest. 1969 Oct;48(10):1895–1904. doi: 10.1172/JCI106156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ROSENBERG L. E., DOWNING S. TRANSPORT OF NEUTRAL AND DIBASIC AMINO ACIDS BY HUMAN LEUKOCYTES: ABSENCE OF DEFECT IN CYSTINURIA. J Clin Invest. 1965 Aug;44:1382–1393. doi: 10.1172/JCI105243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reed P. W. Glutathione and the hexose monophosphate shunt in phagocytizing and hydrogen peroxide-treated rat leukocytes. J Biol Chem. 1969 May 10;244(9):2459–2464. [PubMed] [Google Scholar]
  14. Strauss R. R., Paul B. B., Jacobs A. A., Sbarra A. J. Role of the phagocyte in host-parasite interactions. XXII. H2O2-dependent decarbosylation and deamination by myeloperoxidase and its relationship to antimicrobial activity. J Reticuloendothel Soc. 1970 Jun;7(6):754–761. [PubMed] [Google Scholar]

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