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. 1972 Jun;5(6):902–904. doi: 10.1128/iai.5.6.902-904.1972

Rabies Antibody Determination by Immunofluorescence in Tissue Culture 1

John G Debbie a, Jerome A Andrulonis a, Melvin K Abelseth a
PMCID: PMC422460  PMID: 4628958

Abstract

A tissue culture (TC)-fluorescent antibody (FA) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. This test was performed with BHK-21 cells infected with the ERA vaccine virus strain on Lab-Tek TC chamber slides. A Flury high egg passage (HEP) rabies virus strain grown on continuous line of African green monkey (Vero) and on BHK-21 cells was investigated to determine its utilization in a TC-FA neutralization procedure. Although both the HEP and ERA viruses infected Vero and BHK-21 cells, the amount of fluorescent antigen observed was most consistent with ERA virus and BHK-21 cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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