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. 1972 Jul;6(1):9–16. doi: 10.1128/iai.6.1.9-16.1972

Specific Macrophage Immunity to Vaccinia Virus: Macrophage-Virus Interaction

F R Avila 1, R M Schultz 1, W A F Tompkins 1
PMCID: PMC422482  PMID: 4564156

Abstract

Macrophage immunity to vaccinia virus was examined by measuring replication of vaccinia virus in immune and nonimmune rabbit peritoneal macrophages. Vaccinia virus multiplied in macrophages from normal rabbits but failed to replicate in macrophages from immune rabbits. The specificity of vaccinia restriction was demonstrated by the ability of myxoma virus to replicate in nonimmune and vaccinia-immune macrophages. Vaccinia virus absorbed equally well to immune and nonimmune macrophages, but only a small fraction of immune cells supported synthesis of virus antigen. Virus eclipse and infective center neutralization studies suggested that much of the virus remained at the plasma membrane of immune macrophages, even after prolonged incubation in culture. These data support our previous suggestions that macrophages may contribute to specific immunity against vaccinia virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C. Cell-mediated immune responses to virus infections and virus-induced tumours. Br Med Bull. 1967 Jan;23(1):60–65. doi: 10.1093/oxfordjournals.bmb.a070518. [DOI] [PubMed] [Google Scholar]
  2. Blanden R. V. Mechanisms of recovery from a generalized viral infection: mousepox. I. The effects of anti-thymocyte serum. J Exp Med. 1970 Nov;132(5):1035–1054. doi: 10.1084/jem.132.5.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Glasgow L. A. Leukocytes and interferon in the host response to viral infections. II. Enhanced interferon response of leukocytes from immune animals. J Bacteriol. 1966 Jun;91(6):2185–2191. doi: 10.1128/jb.91.6.2185-2191.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hirsch M. S., Zisman B., Allison A. C. Macrophages and age-dependent resistance to Herpes simplex virus in mice. J Immunol. 1970 May;104(5):1160–1165. [PubMed] [Google Scholar]
  5. MIMS C. A. ASPECTS OF THE PATHOGENESIS OF VIRUS DISEASES. Bacteriol Rev. 1964 Mar;28:30–71. doi: 10.1128/br.28.1.30-71.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mackaness G. B. The monocyte in cellular immunity. Semin Hematol. 1970 Apr;7(2):172–184. [PubMed] [Google Scholar]
  7. NISHMI M., NIECIKOWSKI H. INTERACTION OF VACCINIA VIRUS AND CELLS IN PRIMARY AND CONTINUOUS CULTURE. Nature. 1963 Sep 14;199:1117–1118. doi: 10.1038/1991117a0. [DOI] [PubMed] [Google Scholar]
  8. Neta R., Salvin S. B. Cellular immunity in vitro: migration inhibition and phagocytosis. Infect Immun. 1971 Dec;4(6):697–702. doi: 10.1128/iai.4.6.697-702.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. ROBERTS J. A. GROWTH OF VIRULENT AND ATTENUATED ECTROMELIA VIRUS IN CULTURED MACROPHAGES FROM NORMAL AND ECTROMELIAIMMUNE MICE. J Immunol. 1964 Jun;92:837–842. [PubMed] [Google Scholar]
  10. STEINBERGER A., RIGHTS F. L. EFFECT OF IMMUNIZATION ON TISSUE SUSCEPTIBILITY TO VACCINIA VIRUS IN VITRO. Virology. 1963 Nov;21:402–410. doi: 10.1016/0042-6822(63)90202-2. [DOI] [PubMed] [Google Scholar]
  11. Salvin S. B., Cheng S. L. Lymphoid Cells in Delayed Hypersensitivity II. In Vitro Phagocytosis and Cellular Immunity. Infect Immun. 1971 Apr;3(4):548–552. doi: 10.1128/iai.3.4.548-552.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Silverstein S. Macrophages and viral immunity. Semin Hematol. 1970 Apr;7(2):185–214. [PubMed] [Google Scholar]
  13. Stevens J. G., Cook M. L. Restriction of herpes simplex virus by macrophages. An analysis of the cell-virus interaction. J Exp Med. 1971 Jan 1;133(1):19–38. doi: 10.1084/jem.133.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tompkins W. A., Adams C., Rawls W. E. An in vitro measure of cellular immunity to fibroma virus. J Immunol. 1970 Feb;104(2):502–510. [PubMed] [Google Scholar]
  15. Tompkins W. A., Crouch N. A., Tevethia S. S., Rawls W. E. Characterization of surface antigen on cells infected by fibroma virus. J Immunol. 1970 Nov;105(5):1181–1189. [PubMed] [Google Scholar]
  16. Tompkins W. A., Zarling J. M., Rawls W. E. In vitro assessment of cellular immunity to vaccinia virus: contribution of lymphocytes and macrophages. Infect Immun. 1970 Dec;2(6):783–790. doi: 10.1128/iai.2.6.783-790.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Unanue E. R., Cerottini J. C., Bedford M. Persistence of antigen on the surface of macrophages. Nature. 1969 Jun 21;222(5199):1193–1195. doi: 10.1038/2221193a0. [DOI] [PubMed] [Google Scholar]
  18. Unanue E. R., Cerottini J. C. The function of macrophages in the immune response. Semin Hematol. 1970 Apr;7(2):225–248. [PubMed] [Google Scholar]
  19. Zisman B., Hirsch M. S., Allison A. C. Selective effects of anti-macrophage serum, silica and anti-lymphocyte serum on pathogenesis of herpes virus infection of young adult mice. J Immunol. 1970 May;104(5):1155–1159. [PubMed] [Google Scholar]

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