Abstract
Inoculation of human diploid cells (WI-38) with murine cytomegalovirus (MCMV) did not result in the synthesis of any infectious virions. However, morphological changes typical of the cytopathic effects (CPE) of MCMV were detectable within 12 hr of infection. The CPE included rounding, swelling, and detachment of cells. The nuclei of infected cells were enlarged, and intranuclear inclusions were visible by May Grunwald-Giemsa staining and by the indirect fluorescent-antibody test. All cells infected at a multiplicity of infection of 3 showed CPE, and these cells could not be passaged successfully. Cell lysates and exhausted media from infected WI-38 cultures did not produce any CPE in WI-38 cells. The virus absorbed to WI-38 cells with the same efficiency as to mouse embryo fibroblast cells (MEF). Samples of MCMV in which virus infectivity for MEF cells had been inactivated by ultraviolet irradiation or by exposure to 56 C failed to produce any of the above signs. MCMV-specific CPE did not occur in the presence of actinomycin D (1 μg/ml) or puromycin (20 μg/ml), but 5′-fluoro-2′-deoxyuridine at 1 × 10−4m did not prevent CPE or the development of intranuclear inclusions.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abraham A., Tegtmeyer P. Morphological changes in productive and abortive infection by feline herpesvirus. J Virol. 1970 May;5(5):617–623. doi: 10.1128/jvi.5.5.617-623.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bablanian R. The prevention of early vaccinia-virus-induced cytopathic effects by inhibition of protein synthesis. J Gen Virol. 1968 Jul;3(1):51–61. doi: 10.1099/0022-1317-3-1-51. [DOI] [PubMed] [Google Scholar]
- Kim K. S., Carp R. I. Growth of murine cytomegalovirus in various cell lines. J Virol. 1971 Jun;7(6):720–725. doi: 10.1128/jvi.7.6.720-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lowry S. P., Bronson D. L., Rawls W. E. Characterization of the abortive infection of chick embryo cells by herpesvirus type I. J Gen Virol. 1971 Apr;11(1):47–51. doi: 10.1099/0022-1317-11-1-47. [DOI] [PubMed] [Google Scholar]
- Plummer G. Comparative virology of the herpes group. Prog Med Virol. 1967;9:302–340. [PubMed] [Google Scholar]
- SMITH K. O. RELATIONSHIP BETWEEN THE ENVELOPE AND THE INFECTIVITY OF HERPES SIMPLEX VIRUS. Proc Soc Exp Biol Med. 1964 Mar;115:814–816. doi: 10.3181/00379727-115-29045. [DOI] [PubMed] [Google Scholar]
- Spring S. B., Roizman B. Herpes simplex virus products in productive and abortive infection. I. Stabilization with formaldehyde and preliminary analyses by isopycnic centrifugation in CsCl. J Virol. 1967 Apr;1(2):294–301. doi: 10.1128/jvi.1.2.294-301.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]