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. 1973 Jan;7(1):53–61. doi: 10.1128/iai.7.1.53-61.1973

Radiobacteriolysis: a New Technique Using Chromium-51 for Assaying Anti- Vibrio cholerae Antibodies

Uzy Blachman 1, W R Clark 1, M J Pickett 1
PMCID: PMC422638  PMID: 4570279

Abstract

A new method for detecting and quantitating antibodies against Vibrio cholerae is described. The reaction involves the release of radiochromium from prelabeled vibrios in the presence of specific antibody and complement. The entire assay can be completed within 5 hr. The method is highly reproducible, immunologically specific, temperature- and complement-dependent, and significantly more sensitive than other methods currently used for titration of anti-Vibrio cholerae antibodies. The technique is also potentially applicable to titration of antibodies against other gram-negative bacteria.

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Selected References

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  1. Benenson A. S., Saad A., Mosley W. H. Serological studies in cholera. 2. The vibriocidal antibody response of cholera patients determined by a microtechnique. Bull World Health Organ. 1968;38(2):277–285. [PMC free article] [PubMed] [Google Scholar]
  2. Brunner K. T., Mauel J., Cerottini J. C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968 Feb;14(2):181–196. [PMC free article] [PubMed] [Google Scholar]
  3. Canty T. G., Wunderlich J. R. Quantitative in vitro assay of cytotoxic cellular immunity. J Natl Cancer Inst. 1970 Oct;45(4):761–772. [PubMed] [Google Scholar]
  4. Davis S. D., Gemsa D., Wedgwood R. J. Kinetics of the transformation of Gram-negative rods to spheroplasts and ghosts by serum. J Immunol. 1966 Apr;96(4):570–577. [PubMed] [Google Scholar]
  5. Deb B. C., Sinha R., Shrivastava D. L. Vibriocidal antibody titrations: a comparison of three methods. Indian J Med Res. 1969 Jan;57(1):167–174. [PubMed] [Google Scholar]
  6. GRAY S. J., STERLING K. The tagging of red cells and plasma proteins with radioactive chromium. J Clin Invest. 1950 Dec;29(12):1604–1613. doi: 10.1172/JCI102403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ganguly R., Deb B. C., Shrivastava D. L. Vibriocidal test and its parameters. Indian J Med Res. 1969 May;57(5):815–822. [PubMed] [Google Scholar]
  8. Gemsa D., Davis S. D., Wedgwood R. J. Lysozyme and serum bactericidal action. Nature. 1966 May 28;210(5039):950–951. doi: 10.1038/210950a0. [DOI] [PubMed] [Google Scholar]
  9. Glynn A. A., Milne C. M. A kinetic study of the bacteriolytic and bactericidal action of human serum. Immunology. 1967 Jun;12(6):639–653. [PMC free article] [PubMed] [Google Scholar]
  10. Holmgren J., Svennerholm A. M., Ouchterlony O. Quantitation of vibriocidal antibodies using agar plague techniques. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):708–714. [PubMed] [Google Scholar]
  11. Kasper D. L., Wyle F. A., Formal S. B. Bactericidal antibody assay using 14 C-labeled Neisseria meningitidis. Proc Soc Exp Biol Med. 1972 Apr;139(4):1175–1180. doi: 10.3181/00379727-139-36324. [DOI] [PubMed] [Google Scholar]
  12. MCINTYRE O. R., FEELEY J. C. PASSIVE SERUM PROTECTION OF THE INFANT RABBIT AGAINST EXPERIMENTAL CHOLERA. J Infect Dis. 1964 Dec;114:468–475. doi: 10.1093/infdis/114.5.468. [DOI] [PubMed] [Google Scholar]
  13. MUSCHEL L. H., TREFFERS H. P. Quantitative studies on the bactericidal actions of serum and complement. I. A rapid photometric growth assay for bactericidal activity. J Immunol. 1956 Jan;76(1):1–10. [PubMed] [Google Scholar]
  14. Sack R. B., Barua D., Saxena R., Carpenter C. C. Vibriocidal and agglutinating antibody patterns in cholera patients. J Infect Dis. 1966 Dec;116(5):630–640. doi: 10.1093/infdis/116.5.630. [DOI] [PubMed] [Google Scholar]
  15. Spitznagel J. K., Wilson L. A. Normal serum cytotoxicity for P32-labeled smooth Enterobacteriaceae. I. Loss of label, death, and ultrastructural damage. J Bacteriol. 1966 Jan;91(1):393–400. doi: 10.1128/jb.91.1.393-400.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WARDLAW A. C. The complement-dependent bacteriolytic activity of normal human serum. I. The effect of pH and ionic strength and the role of lysozyme. J Exp Med. 1962 Jun 1;115:1231–1249. doi: 10.1084/jem.115.6.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]

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