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. 1973 Apr;7(4):532–538. doi: 10.1128/iai.7.4.532-538.1973

Mechanism of Shope Fibroma Virus-Induced Suppression of Host Deoxyribonucleic Acid Synthesis

James C Chan a,1, M E Hodes a
PMCID: PMC422717  PMID: 4202660

Abstract

The effects of treatment with live or inactivated Shope fibroma virus on host cell deoxyribonucleic acid (DNA) synthesis were determined. The incorporation of 3H-thymidine into nuclear DNA was suppressed by both active and inactivated virus, although live virus was more effective. During the early phase of infection, stimulation of host nuclear DNA synthesis of up to 240% of control value was observed in cells infected with active virus. Inhibition of DNA synthesis began at about the 8th h and was maximal by 12 h postinfection. Virus inactivated by ultraviolet-irradiation or heat treatment did not induce viral DNA synthesis but was, nevertheless, able to suppress host DNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ewton D., Hodes M. E. Nucleic acid synthesis in HeLa cells infected with Shope fibroma virus. Virology. 1967 Sep;33(1):77–83. doi: 10.1016/0042-6822(67)90095-5. [DOI] [PubMed] [Google Scholar]
  3. JOKLIK W. K., BECKER Y. THE REPLICATION AND COATING OF VACCINIA DNA. J Mol Biol. 1964 Dec;10:452–474. doi: 10.1016/s0022-2836(64)80066-8. [DOI] [PubMed] [Google Scholar]
  4. JOKLIK W. K. The purification fo four strains of poxvirus. Virology. 1962 Sep;18:9–18. doi: 10.1016/0042-6822(62)90172-1. [DOI] [PubMed] [Google Scholar]
  5. Joklik W. K. The poxviruses. Annu Rev Microbiol. 1968;22:359–390. doi: 10.1146/annurev.mi.22.100168.002043. [DOI] [PubMed] [Google Scholar]
  6. Jungwirth C., Launer J. Effect of poxvirus infection on host cell deoxyribonucleic acid synthesis. J Virol. 1968 May;2(5):401–408. doi: 10.1128/jvi.2.5.401-408.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KATO S., TSURU K., MIYAMOTO H. AUTORADIOGRAPHIC STUDIES ON THE YABA MONKEY TUMOR VIRUS AND HOST CELL INTERACTION. Biken J. 1965 Apr;8:45–53. [PubMed] [Google Scholar]
  8. Koziorowska J., Wlodarski K., Mazurowa N. Transformation of mouse emnbryo cells by vaccinia virus. J Natl Cancer Inst. 1971 Feb;46(2):225–241. [PubMed] [Google Scholar]
  9. Mantani M., Miyamoto H., Kato S. Effect of viral DNA synthesis of poxvirus upon nuclear DNA synthesis of synchronized FL cells. Biken J. 1968 Jun;11(2):71–90. [PubMed] [Google Scholar]
  10. McAuslan B. R., Smith W. R. Deoxyribonucleic acid synthesis in FV-3-infected mammalian cells. J Virol. 1968 Oct;2(10):1006–1015. doi: 10.1128/jvi.2.10.1006-1015.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Scherrer R. Viral and host deoxyribonucleic acid synthesis in Shope fibroma virus-infected cells as studied by means of high-resolution autoradiography. J Virol. 1968 Dec;2(12):1418–1428. doi: 10.1128/jvi.2.12.1418-1428.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tompkins W. A., Walker D. L., Hinze H. C. Cellular deoxyribonucleic acid synthesis and loss of contact inhibition in irradiated and contact-inhibited cell cultures infected with fibroma virus. J Virol. 1969 Nov;4(5):603–609. doi: 10.1128/jvi.4.5.603-609.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vesco C., Penman S. Purified cytoplasmic DNA from HeLa cells: resistance to inhibition by hydroxyurea. Biochem Biophys Res Commun. 1969 Apr 29;35(2):249–257. doi: 10.1016/0006-291x(69)90275-7. [DOI] [PubMed] [Google Scholar]
  14. Walen K. H. Nuclear involvement poin xvirus infection. Proc Natl Acad Sci U S A. 1971 Jan;68(1):165–168. doi: 10.1073/pnas.68.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zakay-Rones Z., Becker Y. Anti-poxvirus activity of rifampicin associated with hydrazone side chain. Nature. 1970 Jun 20;226(5251):1162–1163. doi: 10.1038/2261162a0. [DOI] [PubMed] [Google Scholar]

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