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. 1973 Jul;8(1):42–47. doi: 10.1128/iai.8.1.42-47.1973

Relationship Between Tuberculin Hypersensitivity and Cellular Immunity to Infection in Mice Vaccinated with Viable Attenuated Mycobacterial Cells or with Mycobacterial Ribonucleic Acid Preparations

Rochelle G Neiburger 1, Guy P Youmans 1, Anne S Youmans 1
PMCID: PMC422807  PMID: 4198103

Abstract

The migration inhibition technique has been used to study delayed hypersensitivity in vitro by using peritoneal exudate cells and splenic lymphocytes from mice vaccinated with viable cells of the attenuated H37Ra strain of Mycobacterium tuberculosis and from mice vaccinated with ribonucleic acid (myc RNA) preparations obtained from viable mycobacterial cells of the same strain. Inhibition of macrophage migration was noted when purified protein derivative (PPD) or viable H37Ra cells were added to peritoneal exudate cells obtained from mice immunized with viable H37Ra cells and not from mice immunized with myc RNA. Splenic lymphocyte cultures were exposed to the same antigens in vitro. Filtered supernatant fluids from these lymphocyte cultures, when added to peritoneal exudate cells obtained from nonimmunized mice, inhibited migration only when they were obtained from lymphocytes which came from mice immunized with viable H37Ra cells. Injection of PPD intravenously into vaccinated mice resulted in inhibitory supernatant fluids from splenic lymphocyte cultures only when the lymphocytes came from mice immunized with viable H37Ra cells. However, intravenous injection of either viable H37Ra cells or of myc RNA preparations into mice vaccinated with myc RNA occasionally produced inhibitory supernatant fluids when lymphocytes were obtained from these mice. On the other hand, mice vaccinated with myc RNA or viable H37Ra cell preparations were consistently and equally protected against intravenous challenge with the virulent H37Rv strain. Thus, although some evidence was obtained for a delayed type hypersensitivity in mice vaccinated with H37Ra cells or with myc RNA to ribosomal proteins or other proteins associated with the RNA preparation, no evidence of tuberculin hypersensitivity could be detected in any mice vaccinated with the myc RNA. These results argue against a role for tuberculin hypersensitivity in immunity to tuberculous infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker R. E., Hill W. E., Larson C. L. Delayed hypersensitivity reactions provoked by ribosomes from acid-fast bacilli. I. Ribosomal isolation, characterization, delayed hypersensitivity, and specificity. Infect Immun. 1972 Sep;6(3):258–265. doi: 10.1128/iai.6.3.258-265.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coppel S., Youmans G. P. Specificity of Acquired Resistance Produced by Immunization with Listeria monocytogenes and Listeria Fractions. J Bacteriol. 1969 Jan;97(1):121–126. doi: 10.1128/jb.97.1.121-126.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coppel S., Youmans G. P. Specificity of acquired resistance produced by immunization with mycobacterial cells and mycobacterial fractions. J Bacteriol. 1969 Jan;97(1):114–120. doi: 10.1128/jb.97.1.114-120.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coppel S., Youmans G. P. Specificity of the anamnestic response produced by Listeria monocytogenes or Mycobacterium tuberculosis to challenge with Listeria monocytogenes. J Bacteriol. 1969 Jan;97(1):127–133. doi: 10.1128/jb.97.1.127-133.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MACKANESS G. B. THE IMMUNOLOGICAL BASIS OF ACQUIRED CELLULAR RESISTANCE. J Exp Med. 1964 Jul 1;120:105–120. doi: 10.1084/jem.120.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mackaness G. B. The relationship of delayed hypersensitivity to acquired cellular resistance. Br Med Bull. 1967 Jan;23(1):52–54. doi: 10.1093/oxfordjournals.bmb.a070516. [DOI] [PubMed] [Google Scholar]
  7. Neiburger R. G., Youmans G. P. Inhibition of migration of mouse macrophages by tuberculin-sensitive mouse lymphocytes and by mouse migration inhibitory factor. Infect Immun. 1973 Feb;7(2):190–195. doi: 10.1128/iai.7.2.190-195.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ortiz-Ortiz L., Solarolo E. B., Bojalil L. F. Delayed hypersensitivity to ribosomal protein from BCG. J Immunol. 1971 Oct;107(4):1022–1026. [PubMed] [Google Scholar]
  9. Schlossman S. F., Levin H. A., Rocklin R. E., David J. R. The compartmentalization of antigen-reactive lymphocytes in desensitized guinea pigs. J Exp Med. 1971 Sep 1;134(3 Pt 1):741–750. doi: 10.1084/jem.134.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. YOUMANS G. P., YOUMANS A. S. The measurement of the response of immunized mice to infection with Mycobacterium tuberculosis va. hominis. J Immunol. 1957 May;78(5):318–329. [PubMed] [Google Scholar]
  11. Youmans A. S., Youmans G. P. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis. J Bacteriol. 1966 Jun;91(6):2146–2154. doi: 10.1128/jb.91.6.2146-2154.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Youmans A. S., Youmans G. P. Factors affecting immunogenic activity of mycobacterial ribosomal and ribonucleic acid preparations. J Bacteriol. 1969 Jul;99(1):42–50. doi: 10.1128/jb.99.1.42-50.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Youmans A. S., Youmans G. P. Preparation and effect of different adjuvants on the immunogenic activity of mycobacterial ribosomal fraction. J Bacteriol. 1967 Oct;94(4):836–843. doi: 10.1128/jb.94.4.836-843.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Youmans A. S., Youmans G. P. Preparation of highly immunogenic ribosomal fractions of Mycobacterium tuberculosis by use of sodium dodecyl sulfate. J Bacteriol. 1966 Jun;91(6):2139–2145. doi: 10.1128/jb.91.6.2139-2145.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Youmans G. P., Youmans A. S. Allergenicity of mycobacterial ribosomal and ribonucleic acid preparations in mice and guinea pigs. J Bacteriol. 1969 Jan;97(1):134–139. doi: 10.1128/jb.97.1.134-139.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Youmans G. P., Youmans A. S. Nonspecific factors in resistance of mice to experimental tuberculosis. J Bacteriol. 1965 Dec;90(6):1675–1681. doi: 10.1128/jb.90.6.1675-1681.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Youmans G. P., Youmans A. S. Recent studies on acquired immunity in tuberculosis. Curr Top Microbiol Immunol. 1969;48:129–178. doi: 10.1007/978-3-642-46163-7_6. [DOI] [PubMed] [Google Scholar]
  18. Youmans G. P., Youmans A. S. Response of vaccinated and nonvaccinated syngeneic C57B1-6 mice to infection with Mycobacterium tuberculosis. Infect Immun. 1972 Nov;6(5):748–754. doi: 10.1128/iai.6.5.748-754.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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