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. 1973 Sep;8(3):395–400. doi: 10.1128/iai.8.3.395-400.1973

Ribosomal Vaccines II. Specificity of the Immune Response to Ribosomal Ribonucleic Acid and Protein Isolated from Salmonella typhimurium

William Johnson 1
PMCID: PMC422861  PMID: 4199718

Abstract

Ribosomal proteins isolated from Salmonella typhimurium were effective in inducing immunity in mice. This immunity was specific since animals immunized with ribosomal proteins from S. typhimurium were not protected against challenge with S. cholerae-suis or S. enteritidis. Immunity was evident as early as 5 days after immunization. Ribosomal ribonucleic acid (RNA) failed to provide any effective immunity in mice. Polyinosinic acid: polycytidylic acid (poly I: C) induced a rapid, short-lived immunity to all three Salmonella species. In contrast, ribosomal RNA failed to elicit any rapid nonspecific response to infection.

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Selected References

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  1. Fogel S., Sypherd P. S. Extraction and isolation of individual ribosomal proteins from Escherichia coli. J Bacteriol. 1968 Aug;96(2):358–364. doi: 10.1128/jb.96.2.358-364.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hardy S. J., Kurland C. G., Voynow P., Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969 Jul;8(7):2897–2905. doi: 10.1021/bi00835a031. [DOI] [PubMed] [Google Scholar]
  3. Johnson W. Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis. Infect Immun. 1972 Jun;5(6):947–952. doi: 10.1128/iai.5.6.947-952.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kurland C. G. The requirements for specific sRNA binding by ribosomes. J Mol Biol. 1966 Jun;18(1):90–108. doi: 10.1016/s0022-2836(66)80079-7. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Levin J., Bang F. B. Clottable protein in Limulus; its localization and kinetics of its coagulation by endotoxin. Thromb Diath Haemorrh. 1968 Mar 31;19(1):186–197. [PubMed] [Google Scholar]
  7. Margolis J. M., Bigley N. J. Cytophilic macroglobulin reactive with bacterial protein in mice immunized with ribonucleic acid-protein fractions of virulent Salmonella typhimurium. Infect Immun. 1972 Sep;6(3):390–397. doi: 10.1128/iai.6.3.390-397.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Otaka E., Itoh T., Osawa S. Ribosomal proteins of bacterial cells: strain- and species-specificity. J Mol Biol. 1968 Apr 14;33(1):93–107. doi: 10.1016/0022-2836(68)90283-0. [DOI] [PubMed] [Google Scholar]
  9. Patterson R. J., Youmans G. P. Demonstration in tissue culture of lymphocyte-mediated immunity to tuberculosis. Infect Immun. 1970 Jun;1(6):600–603. doi: 10.1128/iai.1.6.600-603.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rudbach J. A. Molecular immunogenicity of bacterial lipopolysaccharide antigens: establishing a quantitative system. J Immunol. 1971 Apr;106(4):993–1001. [PubMed] [Google Scholar]
  11. Sarkar P. K., Som S. Species specificity of ribosomal proteins in eucaryotic and procaryotic organisms. Biochem Biophys Res Commun. 1969 Nov 20;37(5):767–770. doi: 10.1016/0006-291x(69)90957-7. [DOI] [PubMed] [Google Scholar]
  12. Smith R. A., Bigley N. J. Detection of delayed hypersensitivity in mice injected with ribonucleic acid-protein fractions of Salmonella typhimurium. Infect Immun. 1972 Sep;6(3):384–389. doi: 10.1128/iai.6.3.384-389.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith R. A., Bigley N. J. Ribonucleic acid-protein fractions of virulent Salmonella typhimurium as protective immunogens. Infect Immun. 1972 Sep;6(3):377–383. doi: 10.1128/iai.6.3.377-383.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thomas D. W., Weiss E. Response of mice to injection of ribosomal fraction from group B Neisseria meningitidis. Infect Immun. 1972 Sep;6(3):355–363. doi: 10.1128/iai.6.3.355-363.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thompson H. C., Snyder I. S. Protection against pneumococcal infection by a ribosomal preparation. Infect Immun. 1971 Jan;3(1):16–23. doi: 10.1128/iai.3.1.16-23.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Venneman M. R., Berry L. J. Cell-mediated resistance induced with immunogenic preparations of Salmonella typhimurium. Infect Immun. 1971 Oct;4(4):381–387. doi: 10.1128/iai.4.4.381-387.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Venneman M. R., Berry L. J. Experimental salmonellosis: differential passive transfer of immunity with serum and cells obtained from ribosomal and ribonucleic acid-immunized mice. J Reticuloendothel Soc. 1971 May;9(5):491–502. [PubMed] [Google Scholar]
  18. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Venneman M. R., Bigley N. J. Isolation and partial characterization of an immunogenic moiety obtained from Salmonella typhimurium. J Bacteriol. 1969 Oct;100(1):140–148. doi: 10.1128/jb.100.1.140-148.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Venneman M. R. Purification of immunogenically active ribonucleic acid preparations of Salmonella typhimurium: molecular-sieve and anion-exchange chromatography. Infect Immun. 1972 Mar;5(3):269–282. doi: 10.1128/iai.5.3.269-282.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weinstein M. J., Waitz J. A., Came P. E. Induction of resistance to bacterial infections of mice with poly I-poly C. Nature. 1970 Apr 11;226(5241):170–170. doi: 10.1038/226170a0. [DOI] [PubMed] [Google Scholar]
  22. YOUMANS A. S., YOUMANS G. P. IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS. J Bacteriol. 1965 May;89:1291–1298. doi: 10.1128/jb.89.5.1291-1298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Youmans A. S., Youmans G. P. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis. J Bacteriol. 1966 Jun;91(6):2146–2154. doi: 10.1128/jb.91.6.2146-2154.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Youmans A. S., Youmans G. P. Factors affecting immunogenic activity of mycobacterial ribosomal and ribonucleic acid preparations. J Bacteriol. 1969 Jul;99(1):42–50. doi: 10.1128/jb.99.1.42-50.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Youmans A. S., Youmans G. P. Failure of synthetic polynucleotides to affect the immunogenicity of mycobacterial ribonucleic Acid and ribosomal protein preparations. Infect Immun. 1971 Jan;3(1):149–153. doi: 10.1128/iai.3.1.149-153.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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