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. 1973 Sep;8(3):475–481. doi: 10.1128/iai.8.3.475-481.1973

Serology of Streptococcus sanguis: Localization of Antigens with Unlabeled Antisera

Chern Lai 1, Max Listgarten 1, Burton Rosan 1
PMCID: PMC422874  PMID: 4199721

Abstract

A new technique for the electron microscope localization of bacterial surface antigens, using unlabeled antisera, has been developed. This technique, called the “immuno-coating reaction” was applied to serological studies of Streptococcus sanguis, using antisera which had been shown to be specific for this species by gel diffusion analysis. The results of the immuno-coating reaction confirmed the specificity of the antisera for S. sanguis. In addition, the data indicate that some of the antigens responsible for the reaction were proteins which formed filamentous processes on the surface of the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlsson J. A numerical taxonomic study of human oral streptococci. Odontol Revy. 1968;19(2):137–160. [PubMed] [Google Scholar]
  2. Carlsson J. Zooglea-forming streptococci, resembling Streptococcus sanguis, isolated from dental plaque in man. Odontol Revy. 1965;16(4):348–358. [PubMed] [Google Scholar]
  3. Ellen R. P., Gibbons R. J. M protein-associated adherence of Streptococcus pyogenes to epithelial surfaces: prerequisite for virulence. Infect Immun. 1972 May;5(5):826–830. doi: 10.1128/iai.5.5.826-830.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gibbons R. J., Van Houte J., Liljemark W. F. Parameters that effect the adherence of Streptococcus salivarius to oral epithelial surfaces. J Dent Res. 1972 Mar-Apr;51(2):424–435. doi: 10.1177/00220345720510023101. [DOI] [PubMed] [Google Scholar]
  5. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lawn A. M. Simple immunological labelling method for electron microscopy and its application to the study of filamentous appendages of bacteria. Nature. 1967 Jun 10;214(5093):1151–1152. doi: 10.1038/2141151a0. [DOI] [PubMed] [Google Scholar]
  7. Mayyasi S. A., Schidlovsky G., Bulfone L. M., Buscheck F. T. The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 1967 Nov;27(11):2020–2024. [PubMed] [Google Scholar]
  8. Mergenhagen S. E., Bladen H. A., Hsu K. C. Electron microscopic localization of endotoxic lipopolysaccharide in gram-negative organisms. Ann N Y Acad Sci. 1966 Jun 30;133(2):279–291. doi: 10.1111/j.1749-6632.1966.tb52371.x. [DOI] [PubMed] [Google Scholar]
  9. RANTZ L. A., RANDALL E. Use of autoclaved extracts of hemolytic streptococci for serological grouping. Stanford Med Bull. 1955 May;13(2):290–291. [PubMed] [Google Scholar]
  10. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rosan B. Antigens of Streptococcus sanguis. Infect Immun. 1973 Feb;7(2):205–211. doi: 10.1128/iai.7.2.205-211.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SINGER S. J. Preparation of an electron-dense antibody conjugate. Nature. 1959 May 30;183(4674):1523–1524. doi: 10.1038/1831523a0. [DOI] [PubMed] [Google Scholar]
  13. SMITH C. W., METZGER J. F., ZACKS S. I., KASE A. Immune electron microscopy. Proc Soc Exp Biol Med. 1960 Jun;104:336–338. doi: 10.3181/00379727-104-25827. [DOI] [PubMed] [Google Scholar]
  14. Swanson J., Hsu K. C., Gotschlich E. C. Electron microscopic studies on streptococci. I. M antigen. J Exp Med. 1969 Nov 1;130(5):1063–1091. doi: 10.1084/jem.130.5.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van Houte J., Gibbons R. J., Banghart S. B. Adherence as a determinant of the presence of Streptococcus salivarius and Streptococcus sanguis on the human tooth surface. Arch Oral Biol. 1970 Nov;15(11):1025–1034. doi: 10.1016/0003-9969(70)90115-9. [DOI] [PubMed] [Google Scholar]
  16. Virgilio A R., González P C., Munoz S N., Mendoza G S. Ability of Staphylococcus aureus Cells to Bind Normal Human Immunoglobulin G. Infect Immun. 1970 Sep;2(3):342–344. doi: 10.1128/iai.2.3.342-344.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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