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. 1974 Sep;10(3):597–604. doi: 10.1128/iai.10.3.597-604.1974

Measurement of Intracellular Iodophilic Polysaccharide in Two Cariogenic Strains of Streptococcus mutans by Cytochemical and Chemical Methods

J R DiPersio 1, S J Mattingly 1, M L Higgins 1, G D Shockman 1
PMCID: PMC422994  PMID: 4139118

Abstract

Quantitative cytological and chemical methods have been developed to study the intracellular iodophilic polysaccharide (IPS) content of two strains of Streptococcus mutans. The cytological method uses a periodic acid-chlorite treatment of thin sections to increase the affinity of IPS for uranyl and lead salts. This results in the IPS appearing as individual electron-dense granules which can be counted for quantitative studies. As a basis for these quantitative studies, IPS was measured chemically by dissolving whole cells with hot KOH and quantitating spectrophotometrically the amount of iodine-polysaccharide complex formed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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