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. 1974 Oct;10(4):733–741. doi: 10.1128/iai.10.4.733-741.1974

Synergistic Action of Cord Factor and Mycobacterial Sulfatides on Mitochondria

Masahiko Kato 1, Mayer B Goren 2
PMCID: PMC423014  PMID: 4214779

Abstract

The mechanism of a synergistic toxicity of 6,6′-dimycoloyl-α,α′-d-trehalose (cord factor) and 2,3,6,6′-tetraacyl-α,α′-d-trehalose 2′-sulfate (sulfolipid I) for mice was studied. Sulfolipid I was entirely nontoxic, but it markedly accelerated the lethal toxicity of cord factor for mice. In vivo, sulfolipid I affected neither respiration nor accompanying phosphorylation of mouse liver mitochondria, whereas in vitro, it induced a swelling and disruption of mitochondrial membranes and strongly inhibited mitochondrial oxidative phosphorylation. The effect of sulfolipid I on mitochondrial structure and function in vitro was neutralized by bovine serum albumin and various animal sera, whereas that of cord factor and cord factor plus sulfolipid I was not prevented by bovine serum albumin. The simultaneous injection of cord factor and sulfolipid I caused an intensive fragmentation of mitochondria and a marked decrease in respiratory and phosphorylative activity in mitochondria. These data indicate that sulfolipid I can achieve an effective attack on mitochondria in combination with cord factor in vivo and induces heavier damage in mitochondrial structure and function than that produced by cord factor alone.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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